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General Information
Symbol
Dmel\zfh2EAB
Species
D. melanogaster
Name
FlyBase ID
FBal0288420
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of a Mi{Hto-WP} element upstream of zfh2, at coordinate 4:515046 (release 5 genome). The HTO exon in the Mi{Hto-WP} element splices to the zfh2 exon that begins at coordinate 5:536208 . This zfh2 exon consists of 5'UTR, but is in frame with the zfh2 codon in the next exon. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto-WP} element. The fusion protein consists of 3 copies of the T:Zzzz\FLAG tag, the mCherry form of Disc\RFP, linker sequence translated from the zfh2 5'UTR exon (KTPSPVRGNYAIDSINWSNNGNDDGSP) and the zfh2 coding sequence.

Insertion components
Mi{Hto-WP}zfh2EAB
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of zfh2EAB under the control of Scer\GAL4Su(H).GBE (in combination with tub-Gal80[ts]) is sufficient to drive activation and growth of enteroblasts.

Expression of zfh2EAB under the control of Scer\GAL4esg.PU (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to an increase in the cell size of enteroblasts in adult guts when compared to controls.

Expression of zfh2EAB under the control of Scer\GAL4Su(H).GBE (in combination with tub-Gal80[ts]) leads to activation of enteroblasts (by decreasing cell circulatory and increasing number of cells with membrane protrusions); an increase in the cell size and cell volume of enteroblasts; and an increase in the proliferation of intestinal stem cells in adult guts when compared to controls.

Expression of zfh2EAB under the control of Scer\GAL4esg.PU (in combination with tub-Gal80[ts]) leads to formation of multilayered intestinal epithelium in the anterior and posterior midgut when compared to controls. Its expression under the control of Scer\GAL4Su(H).GBE (in combination with tub-Gal80[ts]) also leads to formation of multilayered intestinal epithelium in posterior midgut.

Enteroblast clones expressing zfh2EAB (under the control of Scer\GAL4T80) do not exhibit terminal differentiation when compared to controls.

Expression of one copy of zfh2EAB under the control of Scer\GAL4GMR.PU produces flies that have mildly glazed (lacking clear separation of ommatidia) and rough eyes.

Expression of zfh2EAB under the control of Scer\GAL4Bx-MS1096 severely reduces growth of the wing blade.

Expression of zfh2EAB under the control of Scer\GAL4ptc-559.1 prevents the proper extension of the leg, and the tarsal region is not properly patterned or segmented.

Expression of zfh2EAB under the control of Scer\GAL4ey.PU results in a very reduced eye.

When zfh2EAB-expressing clones are generated in follicle cells during mid-oogenesis under the control of Scer\GAL4Act5C.PP) the nuclei are often displaced apically. Cell polarity is otherwise unaffected. There is increased secretion of eggshell at stage 10 compared with controls.

Expression of zfh2EAB under the control of Scer\GAL4nub-AC-62 results in a reduced wing and a deleted wing hinge.

Flies expressing zfh2EAB under the control of Scer\GAL4salm.EPv have extra bristles in the wing blade.

BrdU incorporation is increased at both sides of the anterior/posterior compartment boundary and reduced in the posterior compartment in the wing discs of third instar larvae expressing zfh2EAB under the control of Scer\GAL4hh.PU.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Expression of zfh2EAB under the control of Scer\GAL4zfh2-LP30 partially rescues the deletion of the wing hinge caused by expression of zfh2dsRNA.Scer\UAS under the control of Scer\GAL4zfh2-LP30.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Generated using the hostile takeover (Hto) system.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
zfh2EAB
Name Synonyms
Secondary FlyBase IDs
    References (5)