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General Information
Symbol
Mi{Hto-WP}
FlyBase ID
FBtp0073090
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Encoded product / tool
Description

The Mi{Hto-WP} element contains UAS regulatory sequences and basal promoter (derived from the P{EP} element), an artificial first exon that encodes two tags (3xTag:FLAG and mCherry) and a 5' splice site downstream of this exon. The artificial exon includes a 5'UTR from the strongly expressed sqh gene. The artificial exon can act as an inducible protein trap cassette upon insertion into the genome either upstream of an endogenous gene or in an intron. When expression is driven from the UAS sequences via a GAL4 driver, the artificial exon can splice out into the genome, forming a fusion transcript with the next available downstream ('target') exon. This may result in tagging of the coding sequence of the targeted exon if the Mi{Hto-WP} insertion is in the correct splice frame. If the insertion is in a coding intron intron, any fusion protein produced may be defective, since it will lack the normal coding sequence from exons that are N-terminal to the Mi{Hto-WP} insertion.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
(bases )
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
molecular
tag
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (14)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
Secondary FlyBase IDs
    References (7)