FlyBase Allele Report: Dmel\RhoGEF64C[EP3035]

General Information

Symbol

Dmel\RhoGEF64C^EP3035

Species

D. melanogaster

Name

FlyBase ID

FBal0136060

Feature type

allele

Associated gene

Dmel\RhoGEF64C

Associated Insertion(s)

P{EP}EP3035

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(McGovern et al., 2003, Pena-Rangel et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{EP}EP3035

Cytology

Description

Inserted 91bp downstream from the start of the Gef64C first exon.

(McGovern et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:4,692,823..4,692,823 [+]

(Raymond et al., 2004, McGovern et al., 2003, Pena-Rangel et al., 2002, Bashaw et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

viable, with Scer\GAL4^sca-4512

(McGovern et al., 2003)

Phenotype Manifest In

larval longitudinal connective, with Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

larval ventral nerve cord commissure, with Scer\GAL4^sca-4512

(McGovern et al., 2003)

symmetrical commissure, with Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

Detailed Description

Statement

Reference

ventral nerve cord commissures in stage 17 Gef64C^EP3035; Scer\GAL4^sca-4512 embryos are fused.

(McGovern et al., 2003)

Expression of Gef64C^EP3035 under the control of Scer\GAL4^elav.PLu results in many axons abnormally projecting across the midline. The commissures are thicker than normal and there is a reduction in the longitudinal axon tracts.

(Bashaw et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^elav.PLu has abnormal neuroanatomy phenotype, suppressible by Rho1^N19.UAS, Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

Enhancer of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of RacGAP84C^GAP.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Scer\GAL4^pnr-MD237/RhoGEF64C^EP3035 is an enhancer of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

NOT Enhancer of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Cdc42^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Suppressor of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^elav.PLu is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4^elav.PLu, robo1^Y-F.UAS

(Bashaw et al., 2001)

NOT Suppressor of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Cdc42^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^elav.PLu has axon phenotype, suppressible by Rho1^N19.UAS, Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

Enhancer of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of RacGAP84C^GAP.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Scer\GAL4^pnr-MD237/RhoGEF64C^EP3035 is an enhancer of chaeta phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

NOT Enhancer of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Cdc42^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Suppressor of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^elav.PLu is a suppressor | partially of symmetrical commissure phenotype of Scer\GAL4^elav.PLu, robo1^Y-F.UAS

(Bashaw et al., 2001)

NOT Suppressor of

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Cdc42^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

RhoGEF64C^EP3035, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Other

Statement

Reference

RhoGEF64C^EP3035, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has macrochaeta phenotype

(Pena-Rangel et al., 2002)

RhoGEF64C^EP3035, Scer\GAL4^pnr-MD237, pnr^MD237 has macrochaeta phenotype

(Pena-Rangel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

pnr^MD237/+ adults expressing Gef64C^EP3035 under the control of Scer\GAL4^pnr-MD237 have deformed macrochaetae.

(Pena-Rangel et al., 2002)

Gef64C^EP3035 dramatically enhances the axon guidance defects caused by expression of fra::robo^{Scer\UAS.RF.T:Hsap\MYC} under the control of Scer\GAL4^elav.PLu, leading to a significant increase in ectopic midline crossing. Co-expression of Rac1^N17.Scer\UAS has little or no effect on the gain of function axon guidance defects caused by expression of Gef64C^EP3035 under the control of Scer\GAL4^elav.PLu. Co-expression of Cdc42^N17.Scer\UAS has little or no effect on the Co-expression of Rho1^N19.Scer\UAS strongly suppresses the gain of function axon guidance defects caused by expression of Gef64C^EP3035 Many commissural axons cross the midline, with some segments appearing nearly wild type, in embryos coexpressing robo^Y-F.Scer\UAS and Gef64C^EP3035 under the control of Scer\GAL4^elav.PLu.

(Bashaw et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EP3035^EP3035

(Pena-Rangel et al., 2002)

Gef64C^EP3035

RhoGEF64C^EP3035

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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