At 50 hours after clone induction osa308 mutant clones contain a single type II NB, indistinguishable from controls. However the number of immature Intermediate Neural Progenitors (imINPs) is significantly increased compared to controls. By seventy five hours after clone induction, multiple type II NB-like cells are seen, compared to only one in control clones, and these are several cell diameters away from the primary NB. The number of imINPs is no longer significantly different from controls and there is no significant difference in the numbers of mature Intermediate Neural Progenitors (mINPs) numbers
Somatic clones of osa308 tissue induced in a non-Minute background proliferate poorly in the wing disc, and can not be recovered in the adult wing. Growth of osa308 cells is rescued in a Minute[+] background. A fraction of these cells localise to the basal side of the wing blade epithelium, are extruded basally, and undergo cell death. These animals die as pupae.
osa308 homozygous mutant clones generated in the entire wing blade result in wings that are very reduced in size, lacking all the veins and most structures of the wing margin. This phenotype is cell-autonomous.
osa308 homozygous mutant clones generated in the central region of the wing blade results in basal extrusion of some wing blade epithelial cells and cell death. Adult wings develop no distal veins, and have ectopic hairs and sensory organs. This phenotype is cell-autonomous.
osa308 homozygous mutant clones generated in the tarsal segments of the developing leg results in fusion of the adult tarsal segments, and loss of the distal-most elements.
In osa308 clones in the eye disc a reduced number of photoreceptors differentiate.
Dominant held out wing phenotype. Clones lead to scars and loss of retinal differentiation.
Homozygous mutant somatic clones can induce leg duplications an the ventral compartment of the leg.
Mosaic clones in the eye disc are rarely recovered, existing clones are associated with scars and within the clone there are fewer than normal photoreceptor clusters and within each cluster there are fewer than normal neuronal cells. Clones that include the posterior margin are frequently much larger than internal clones. Clones induced on the wing disc produced early in development transforms the posterior notum into a partial second wing. The wings have reversed anterior-posterior polarity. Clones induced later in development are associated with ectopic wing margin bristles near the wing margin restricted to the dorsal surface of the anterior compartment. Embryos derived from germline clones show severe defects in the cuticle pattern with many denticle belts either missing, fused or otherwise abnormal. Provision of a paternal wild type copy of osa fails to rescue the mutants.