FlyBase Allele Report: Dmel\osa[308]

General Information

Symbol

Dmel\osa³⁰⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0062861

Feature type

allele

Associated gene

Dmel\osa

Associated Insertion(s)

Carried in Construct

Also Known As

osa^eld308

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Treisman et al., 1997)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle | germline clone

(Treisman et al., 1997)

eye | somatic clone

(Janody et al., 2004, Chanut et al., 2000)

eye-antennal disc | somatic clone

(Treisman et al., 1997)

eye disc | somatic clone

(Janody et al., 2004)

leg | somatic clone | increased number

(Collins and Treisman, 2000)

leg sensillum | somatic clone | increased number

(Collins and Treisman, 2000)

leg ventral compartment | somatic clone

(Collins and Treisman, 2000)

mesonotum | somatic clone

(Treisman et al., 1997)

photoreceptor neuron | somatic clone

(Janody et al., 2004)

retina | somatic clone

(Chanut et al., 2000)

tarsal segment | somatic clone

(Terriente-Félix and de Celis, 2009)

type II neuroblast | somatic clone | increased number

(Eroglu et al., 2014)

ventral denticle belt | germline clone

(Treisman et al., 1997)

wing

(Chanut et al., 2000)

wing | ectopic

(Treisman et al., 1997)

wing blade | cell autonomous | somatic clone

(Terriente-Félix and de Celis, 2009)

wing blade anterior compartment | dorsal | somatic clone

(Treisman et al., 1997)

wing disc | somatic clone

(Terriente-Félix and de Celis, 2009, Treisman et al., 1997)

wing hair | cell autonomous | ectopic | somatic clone

(Terriente-Félix and de Celis, 2009)

wing margin | cell autonomous | somatic clone

(Terriente-Félix and de Celis, 2009)

wing margin bristle | ectopic | somatic clone

(Treisman et al., 1997)

wing sensillum | cell autonomous | ectopic | somatic clone

(Terriente-Félix and de Celis, 2009)

wing vein | cell autonomous | somatic clone

(Terriente-Félix and de Celis, 2009)

Detailed Description

Statement

Reference

At 50 hours after clone induction osa³⁰⁸ mutant clones contain a single type II NB, indistinguishable from controls. However the number of immature Intermediate Neural Progenitors (imINPs) is significantly increased compared to controls. By seventy five hours after clone induction, multiple type II NB-like cells are seen, compared to only one in control clones, and these are several cell diameters away from the primary NB. The number of imINPs is no longer significantly different from controls and there is no significant difference in the numbers of mature Intermediate Neural Progenitors (mINPs) numbers

(Eroglu et al., 2014)

Somatic clones of osa³⁰⁸ tissue induced in a non-Minute background proliferate poorly in the wing disc, and can not be recovered in the adult wing. Growth of osa³⁰⁸ cells is rescued in a Minute[+] background. A fraction of these cells localise to the basal side of the wing blade epithelium, are extruded basally, and undergo cell death. These animals die as pupae.

osa³⁰⁸ homozygous mutant clones generated in the entire wing blade result in wings that are very reduced in size, lacking all the veins and most structures of the wing margin. This phenotype is cell-autonomous.

osa³⁰⁸ homozygous mutant clones generated in the central region of the wing blade results in basal extrusion of some wing blade epithelial cells and cell death. Adult wings develop no distal veins, and have ectopic hairs and sensory organs. This phenotype is cell-autonomous.

osa³⁰⁸ homozygous mutant clones generated in the tarsal segments of the developing leg results in fusion of the adult tarsal segments, and loss of the distal-most elements.

(Terriente-Félix and de Celis, 2009)

In osa³⁰⁸ clones in the eye disc a reduced number of photoreceptors differentiate.

(Janody et al., 2004)

Dominant held out wing phenotype. Clones lead to scars and loss of retinal differentiation.

(Chanut et al., 2000)

Homozygous mutant somatic clones can induce leg duplications an the ventral compartment of the leg.

(Collins and Treisman, 2000)

Mosaic clones in the eye disc are rarely recovered, existing clones are associated with scars and within the clone there are fewer than normal photoreceptor clusters and within each cluster there are fewer than normal neuronal cells. Clones that include the posterior margin are frequently much larger than internal clones. Clones induced on the wing disc produced early in development transforms the posterior notum into a partial second wing. The wings have reversed anterior-posterior polarity. Clones induced later in development are associated with ectopic wing margin bristles near the wing margin restricted to the dorsal surface of the anterior compartment. Embryos derived from germline clones show severe defects in the cuticle pattern with many denticle belts either missing, fused or otherwise abnormal. Provision of a paternal wild type copy of osa fails to rescue the mutants.

(Treisman et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

osa[+]/osa³⁰⁸ is an enhancer of abnormal neuroanatomy phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

osa[+]/osa³⁰⁸ is an enhancer of visible | dominant phenotype of Bx¹

(Bejarano et al., 2008, Milan et al., 2004)

osa³⁰⁸ is an enhancer of visible phenotype of Scer\GAL4^bi-omb-Gal4, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of visible phenotype of Scer\GAL4^vg.PM, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

Suppressor of

Statement

Reference

osa[+]/osa³⁰⁸ is a suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

osa[+]/osa³⁰⁸ is a suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

osa[+]/osa³⁰⁸ is a suppressor of lethal | pupal stage phenotype of Ras85D^V12.UAS, Scer\GAL4^MD-638

(Terriente-Félix and de Celis, 2009)

osa[+]/osa³⁰⁸ is a suppressor | partially of visible | pupal stage phenotype of Ras85D^V12.UAS, Scer\GAL4^MD-638

(Terriente-Félix and de Celis, 2009)

osa[+]/osa³⁰⁸ is a suppressor of abnormal eye color phenotype of Hsap\MECP2^R294X.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

osa[+]/osa³⁰⁸ is a suppressor of visible phenotype of Bx^hdpR590

(Milan et al., 2004)

osa[+]/osa³⁰⁸ is a suppressor of visible phenotype of ro^DOM

(Chanut et al., 2000)

osa³⁰⁸/osa³⁰⁸ is a suppressor | partially | somatic clone of abnormal size | somatic clone | larval stage phenotype of mor¹

(Collins et al., 1999)

Other

Statement

Reference

gl(3R)53-6^3R-53-6, osa³⁰⁸ has viable phenotype

(Luschnig et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

osa[+]/osa³⁰⁸ is an enhancer of type II neuroblast | increased number phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

osa[+]/osa³⁰⁸ is an enhancer of wing vein phenotype of Ras85D^N17.UAS, Scer\GAL4^MD-638

(Terriente-Félix and de Celis, 2009)

osa[+]/osa³⁰⁸ is an enhancer of wing vein phenotype of Scer\GAL4^nub-AC-62, net^UAS.cBa

(Terriente-Félix and de Celis, 2009)

osa[+]/osa³⁰⁸ is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^salm-459.2, rl^Sem.cUa.UAS

(Terriente-Félix and de Celis, 2009)

osa[+]/osa³⁰⁸ is an enhancer of wing margin phenotype of Bx¹

(Bejarano et al., 2008, Milan et al., 2004)

osa³⁰⁸ is an enhancer of wing vein phenotype of Scer\GAL4^bi-omb-Gal4, osa^RD.UAS

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of wing phenotype of Scer\GAL4^bi-omb-Gal4, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^bi-omb-Gal4, osa^RD.UAS

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of wing sensillum | ectopic phenotype of Scer\GAL4^bi-omb-Gal4, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^bi-omb-Gal4, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of wing vein phenotype of Scer\GAL4^bi-omb-Gal4, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of wing phenotype of Scer\GAL4^vg.PM, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of wing margin bristle phenotype of Scer\GAL4^vg.PM, brm^{K804R.UAS.Tag:HA}

(Collins et al., 1999)

osa³⁰⁸ is an enhancer of eye phenotype of dpp^d-blk

(Treisman et al., 1997)

NOT Enhancer of

Statement

Reference

osa[+]/osa³⁰⁸ is a non-enhancer of leg phenotype of e(y)3⁴

(Chalkley et al., 2008)

Suppressor of

Statement

Reference

osa[+]/osa³⁰⁸ is a suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

osa[+]/osa³⁰⁸ is a suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

osa[+], brm[+], osa³⁰⁸, brm² is a suppressor of wing vein | ectopic phenotype of bs²

(Terriente-Félix and de Celis, 2009)

osa[+], brm[+], osa³⁰⁸, brm² is a suppressor of wing vein | ectopic phenotype of net¹, px⁷²

(Terriente-Félix and de Celis, 2009)

osa[+]/osa³⁰⁸ is a suppressor of eye phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

osa[+]/osa³⁰⁸ is a suppressor of wing vein | increased number phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^cv-c-C5

(Cukier et al., 2008)

osa[+]/osa³⁰⁸ is a suppressor of eye phenotype of Hsap\MECP2^Δ166.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

osa[+]/osa³⁰⁸ is a suppressor of wing margin phenotype of Bx^hdpR590

(Milan et al., 2004)

osa[+]/osa³⁰⁸ is a suppressor of eye phenotype of ro^DOM

(Chanut et al., 2000)

osa³⁰⁸ is a suppressor of wing margin phenotype of Scer\GAL4^vg.PM, sgg^S9A.UAS

(Collins and Treisman, 2000)

osa³⁰⁸ is a suppressor of wing phenotype of Scer\GAL4^vg.PM, sgg^S9A.UAS

(Collins and Treisman, 2000)

osa³⁰⁸/osa³⁰⁸ is a suppressor | partially | somatic clone of eye disc | somatic clone phenotype of mor¹

(Collins et al., 1999)

osa³⁰⁸ is a suppressor of eye phenotype of ro^DOM

(Treisman et al., 1997)

NOT Suppressor of

Statement

Reference

osa[+]/osa³⁰⁸ is a non-suppressor of wing vein | ectopic phenotype of Ras85D^V12.UAS, Scer\GAL4^MD-638

(Terriente-Félix and de Celis, 2009)

Other

Statement

Reference

akirin^KG01343, osa[+]/osa³⁰⁸ has larval somatic muscle cell phenotype

(Nowak et al., 2012)

gl(3R)53-6^3R-53-6, osa³⁰⁸ has wing phenotype

(Luschnig et al., 2004)

gro^E48, osa³⁰⁸ has wing | ectopic phenotype

(Collins and Treisman, 2000)

gro^E48, osa³⁰⁸ has mesonotum phenotype

(Collins and Treisman, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is ameliorated by combination with a single copy of osa³⁰⁸.

(Morimoto et al., 2016)

One copy of osa³⁰⁸ enhances the supernumerary neuroblast phenotype seen in brat^DG19310/brat¹¹ mutants.

(Janssens et al., 2014)

Stage 16 osa³⁰⁸/+ ; akirin^KG01343/+ double heterozygous embryos show disruption of the somatic muscle pattern (42% of embryos have missing, misattached or duplicated muscles in at least 2 hemisegments).

(Nowak et al., 2012)

The ectopic wing vein phenotype observed in homozygous bs² mutant wings is suppressed in animals doubly heterozygous for osa³⁰⁸ and brm².

net¹, px⁷² double mutants develop ectopic wing veins in the L2/L3 and L4/L5 intervein regions. This phenotype is suppressed in flies double heterozygous for osa³⁰⁸ and brm².

The loss of wing vein observed by overexpression of Ras85D^N17.UAS under the control of Scer\GAL4^MD-638 is enhanced by osa³⁰⁸/+.

The effects on wing shape and size in animals expressing Ras85D^V12.UAS under the control of Scer\GAL4^MD-638 are partially rescued in a osa³⁰⁸/+ background, although these wings still show ectopic vein differentiation.

The formation of ectopic wing veins in flies expressing rl^Sem.cUa.UAS under the control of Scer\GAL4^salm-459.2 is weakly enhanced in a osa³⁰⁸/+ mutant background.

(Terriente-Félix and de Celis, 2009)

Bap170^Δ65 females with osa³⁰⁸ polybromo^Δ86 germ lines clones are able to differentiate oocytes.

(Carrera et al., 2008)

The severity of the Bx¹/+ wing scalloping phenotype is enhanced by one copy of osa³⁰⁸.

(Milan et al., 2004)

Strong suppressor of ro^DOM.

(Chanut et al., 2000)

gro^E48/osa³⁰⁸ transheterozygous adults have notum to wing transformations.

(Collins and Treisman, 2000)

The addition of osa³⁰⁸ strongly enhances the wing phenotypes seen in brm^{K804R.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^bi-omb-Gal4 flies i.e. loss of the distal wing margin, formation of ectopic campaniform sensillae and wing margin bristles and disruptions of wing vein morphology. osa³⁰⁸ also enhances the wing margin loss phenotype seen in brm^{K804R.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^vg.PM flies. The addition of osa³⁰⁸ enhances the ectopic wing margin bristle and wing vein phenotypes caused by Scer\GAL4^bi-omb-Gal4 driven expression of en::osa^{osaRD.Scer\UAS}. The growth phenotype seen in mor¹ somatic clones is partially rescued by osa³⁰⁸.

(Collins et al., 1999)

Df(3R)Espl22, osa³⁰⁸ double mutant clones in the eye show excess neuronal differentiation.

(Treisman et al., 1997)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is ameliorated by combination with a single copy of osa³⁰⁸.

(Morimoto et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5949

w^*; P{neoFRT}82B osa³⁰⁸/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

eld(308)

(Luschnig et al., 2004)

eld³⁰⁸

(Thomas and Wassarman, 1999)

osa 308

(Cukier et al., 2008)

osa³⁰⁸

(Bose et al., 2025, Niederhuber et al., 2024, Tian and Smith-Bolton, 2021, Eroglu et al., 2014, Janssens et al., 2014, Nowak et al., 2012, Tokusumi et al., 2012, Terriente-Félix and de Celis, 2009, Carrera et al., 2008, Chalkley et al., 2008, Melicharek et al., 2008)

osa^eld308

(Martinho et al., 2004, Milan et al., 2004, Collins and Treisman, 2000, Collins et al., 1999)

osa^eld380

(Bejarano et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (23)

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