FlyBase Allele Report: Dmel\net[1]

General Information

Symbol

Dmel\net¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012933

Feature type

allele

Associated gene

Dmel\net

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

loss of function allele

(Brentrup et al., 2000)

Mutagen

spontaneous

Progenitor genotype

Cytology

Description

244bp duplication between nucleotide position 1105 and 1106 (corresponding to a position between amino acids 277 and 278 of the wild type sequence) with the preceding 244bp duplicated.

(Brentrup et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Nagel et al., 2016, Verheyen et al., 2001)

visible (with Df(2L)ED5878)

(Miller et al., 2020)

visible (with Df(2L)Exel6001)

(Miller et al., 2020, Kahsai and Cook, 2018)

visible (with Df(2L)net62)

(Miller et al., 2020)

visible (with Df(2L)PM4)

(Miller et al., 2020)

visible (with Df(2L)PM44)

(Miller et al., 2020)

visible (with Df(2L)PMA)

(Miller et al., 2020)

visible (with Df(2L)PMD)

(Miller et al., 2020)

visible | recessive

(Brentrup et al., 2000, Baonza and Garcia-Bellido, 1999)

Phenotype Manifest In

posterior wing cell

(Angulo et al., 2004)

submarginal wing cell

(Angulo et al., 2004)

wing vein

(Verheyen et al., 2001, Baonza and Garcia-Bellido, 1999)

wing vein (with Df(2L)ED5878)

(Miller et al., 2020)

wing vein (with Df(2L)Exel6001)

(Miller et al., 2020, Kahsai and Cook, 2018)

wing vein (with Df(2L)net62)

(Miller et al., 2020)

wing vein (with Df(2L)PM4)

(Miller et al., 2020)

wing vein (with Df(2L)PM44)

(Miller et al., 2020)

wing vein (with Df(2L)PMA)

(Miller et al., 2020)

wing vein (with Df(2L)PMD)

(Miller et al., 2020)

wing vein | adult stage | ectopic

(Nagel et al., 2016)

wing vein | ectopic

(Angulo et al., 2004, Marenda et al., 2004, Brentrup et al., 2000)

Detailed Description

Statement

Reference

net¹/Df(2L)Exel6001 results in extra wing veins.

(Kahsai and Cook, 2018)

The net¹ mutants display ectopic veins and networking of veins in the adult wings.

(Nagel et al., 2016)

net¹ homozygotes develop extra vein tissue, mainly in the form of ectopic transverse connections between L2 and L3 and between L4 and L5.

(Angulo et al., 2004)

Heterozygotes have essentially normal wings, occasionally having a single extra vein near the posterior crossvein (3%).

(Marenda et al., 2004)

Homozygotes show ectopic wing veins and plexi.

(Brentrup et al., 2000)

Homozygotes have extra wing veins.

(Baonza and Garcia-Bellido, 1999)

Appearance of distal extra longitudinal veins preferentially associated with plexi between LII and LIII on one side and between LIV and LV on the other.

(Diaz-Benjumea and Garcia-Bellido, 1990)

Wing veins form plexus-like net; first posterior cell between L3 and L4 widens toward tip; branch missing from posterior crossvein; all veins fused at base of wing, like bi. According to Waddington (1940), spaces form between epithelial layers owing to inadequate contraction during pupal period; spaces later fuse and form extra veins (Diaz-Benjumea, Gonzalez-Gaitan, and Garcia-Bellido, 1989). RK1.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

net¹ has visible phenotype, enhanceable by CycG^HR7/CycG^HR7

(Nagel et al., 2016)

net¹ has visible phenotype, enhanceable by nmo^adk2

(Verheyen et al., 2001)

net¹ has visible | recessive phenotype, enhanceable by emc¹/emc[+]

(Baonza and Garcia-Bellido, 1999)

Suppressed by

Statement

Reference

net¹ has visible | recessive phenotype, suppressible by emc^D

(Baonza and Garcia-Bellido, 1999)

Enhancer of

Statement

Reference

net¹/net[+] is an enhancer of visible | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

net¹ is an enhancer of visible phenotype of nmo^adk2

(Verheyen et al., 2001)

Other

Statement

Reference

H^C2.hs, net¹ has partially lethal - majority die | heat sensitive phenotype

(Johannes and Preiss, 2002)

emc¹/emc¹¹, net¹ has visible | dominant phenotype

(Baonza and Garcia-Bellido, 1999)

emc¹/emc¹¹, net¹ has visible phenotype

(Baonza and Garcia-Bellido, 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

net¹ has wing vein | adult stage | ectopic phenotype, enhanceable by CycG^HR7/CycG^HR7

(Nagel et al., 2016)

net¹ has wing vein | adult stage phenotype, enhanceable by ru¹

(Nagel et al., 2016)

net¹ has wing vein | adult stage phenotype, enhanceable by speck¹

(Nagel et al., 2016)

net¹ has wing vein | ectopic phenotype, enhanceable by Df(1)C246/+

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, enhanceable by Df(3L)ZN47

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, enhanceable by HDAC1⁰⁴⁵⁵⁶/Rpd3[+]

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, enhanceable by Snr1^E1/Snr1[+]

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, enhanceable by mor[+]/mor¹

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, enhanceable by osa²/osa[+]

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, enhanceable by Snr1^R3/Snr1[+]

(Marenda et al., 2004)

net¹ has wing vein phenotype, enhanceable by nmo^adk2

(Verheyen et al., 2001)

net¹ has wing vein | ectopic phenotype, enhanceable by emc¹/emc[+]

(Baonza and Garcia-Bellido, 1999)

NOT Enhanced by

Statement

Reference

net¹ has wing vein | ectopic phenotype, non-enhanceable by brm[+]/brm²

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, non-enhanceable by osa⁰⁰⁰⁹⁰/osa[+]

(Marenda et al., 2004)

Suppressed by

Statement

Reference

net¹, px⁷² has wing vein | ectopic phenotype, suppressible by osa[+]/brm[+]/osa³⁰⁸/brm²

(Terriente-Félix and de Celis, 2009)

net¹ has wing vein | ectopic phenotype, suppressible by emc^D

(Baonza and Garcia-Bellido, 1999)

NOT suppressed by

Statement

Reference

net¹ has wing vein | ectopic phenotype, non-suppressible by brm[+]/brm²

(Marenda et al., 2004)

net¹ has wing vein | ectopic phenotype, non-suppressible by osa⁰⁰⁰⁹⁰/osa[+]

(Marenda et al., 2004)

net¹ has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Enhancer of

Statement

Reference

net¹/net[+] is an enhancer of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

net¹/net[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

net¹/net[+] is an enhancer of wing | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

net¹ is an enhancer of wing vein phenotype of nmo^adk2

(Verheyen et al., 2001)

Other

Statement

Reference

net¹, px⁷² has wing vein | ectopic phenotype

(Terriente-Félix and de Celis, 2009)

emc¹/emc¹¹, net¹ has wing vein | ectopic phenotype

(Baonza and Garcia-Bellido, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The net¹ phenotype (ectopic veins, vein networking) can be enhanced by combination with CycG^HR7 but also by combination with mutant alleles of unrelated factors roughoid ru¹ or speck sp¹ demonstrating high susceptibility of the net¹ phenotype to perturbed genetic background.

(Nagel et al., 2016)

net¹, px⁷² double mutants develop ectopic wing veins in the L2/L3 and L4/L5 intervein regions. This phenotype is suppressed in flies double heterozygous for osa³⁰⁸ and brm².

(Terriente-Félix and de Celis, 2009)

net¹ homozygotes develop extra vein tissue, mainly in the form of ectopic transverse connections between L2 and L3 and between L4 and L5. This phenotype is enhanced by ash2^I1/+ which also have a reduction in the size of the intervein regions between L2 and L3 (submarginal cell) and between L4 and L5 (discal cell and second posterior cell). The resulting wings have a lanceolate shape.

(Angulo et al., 2004)

net ; H^C2.hs homozygotes are semi-lethal at 25^oC (and viable at 18^oC). The animals have massive patches of vein tissue and extensive blistering of the wing.

(Johannes and Preiss, 2002)

net¹ nmo^adk2 double mutant flies show a strong synergistic interaction with respect to the extra wing vein phenotype of each single mutant.

(Verheyen et al., 2001)

The extra wing vein phenotype of net¹ homozygotes is suppressed by emc^D and enhanced by emc¹/+. net¹/+ ; emc¹/emc¹¹ flies show an extra wing vein phenotype.

(Baonza and Garcia-Bellido, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

net⁴

(Meyer, 1956)

Partially rescued by

net¹ is partially rescued by net^UAS.cBa/Scer\GAL4^Bx-MS1096

(Brentrup et al., 2000)

Comments