FlyBase Allele Report: Dmel\CycG[HR7]

Duplication at the CycG locus, with a frame-shift mutation in each copy of the gene. The distal gene copy carries an additional deletion distal to the BamHI site of approximately 350bp. A w^+mW.hs marker has been inserted between the two copies as part of the targeting event.

(Nagel et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

CycG^HR7 heterozygous females lay eggs with normal dorsal appendages when compared to controls.

(Bayer et al., 2020)

CycG^HR7/CycG^HR7 larvae have a similar phenotype to starved wild-type controls, even though fed: high fat content, with aggregations of large lipid droplets in the fat body and oenocytes, and signs of autophagy in the fat body.

(Fischer et al., 2016)

CycG^HR7/CycG^HR7 mutants do not show abnormal wing phenotypes.

(Nagel et al., 2016)

CycG^HR7 females lay significantly fewer eggs per day than controls.

CycG^HR7 homozygous mutants are developmentally delayed, smaller and lighter than wild-type controls but their food ingestion at larval stage is normal.

The number and size of cells in the adult wings of CycG^HR7 homozygotes is reduced compared to wild-type.

Clones of cells homozygous mutant for CycG^HR7 are smaller than their wild-type twinspot counterparts in both eye-antennal and wing discs.

CycG^HR7 homozygotes have decreased lifespan upon wet starvation compared to wild-type controls.

CycG^HR7 homozygote larvae are lighter and accumulate more fat than wild-type controls. The fat body of CycG^HR7 homozygote larvae shows an aggregation of lipid droplets, similar to starved wild-type larvae, even when fed.

Oenocytes of the CycG^HR7/CycG^HR7 larvae accumulate lipid droplets regardless of their fed/starved status.

The salivary glands of CycG^HR7 mutant larvae are smaller than in wild-type controls and have smaller nuclei.

CycG^eoC/CycG^HR7 transheterozygotes show developmental delay, reduced body size and weight, higher fat content and decreased egg laying rate.

(Fischer et al., 2015)

Homozygous females are sterile and produce ventralised eggs that have fused dorsal appendages. Eggs derived from homozygous female germline clones develop eggs with a similar phenotype.

Mutant oocytes show defects in karyosome morphology: the DNA is attached to the oocyte nuclear membrane instead of being localised to the centre of the nucleus in 53% of egg chambers.

(Nagel et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

CycG^HR7 has decreased body size | adult stage phenotype, non-enhanceable by wrd^KO2/wrd^KO2

(Fischer et al., 2016)

Suppressed by

Statement

Reference

CycG^HR7 has decreased body size | adult stage phenotype, suppressible by wdb¹⁴/wdb^dw

(Fischer et al., 2016)

CycG^HR7 has abnormal body size | adult stage phenotype, suppressible by wdb¹⁴/wdb^dw

(Fischer et al., 2015)

NOT suppressed by

Statement

Reference

CycG^HR7 has decreased body size | adult stage phenotype, non-suppressible by wrd^KO2/wrd^KO2

(Fischer et al., 2016)

Enhancer of

Statement

Reference

CycG^HR7/CycG^HR7 is an enhancer of visible phenotype of net¹

(Nagel et al., 2016)

CycG^HR7 is an enhancer of visible phenotype of rho^ve-1

(Nagel et al., 2016)

CycG^HR7/CycG^HR7 is an enhancer of visible | dominant phenotype of Delta^B2

(Nagel et al., 2016)

CycG^HR7/CycG^HR7 is an enhancer of visible | dominant phenotype of H^P8

(Nagel et al., 2016)

Suppressor of

Statement

Reference

CycG^HR7 is a suppressor of visible phenotype of vn¹

(Nagel et al., 2016)

Other

Statement

Reference

CycG^HR7, Df(1)N-54l9/+ has visible phenotype

(Nagel et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

CycG^HR7 has dorsal appendage | maternal effect phenotype, enhanceable | maternal effect by spn-B²/spn-B^BU

(Nagel et al., 2012)

Suppressed by

Statement

Reference

CycG^HR7 has embryonic/larval oenocyte phenotype, suppressible by wrd^KO2/wrd^KO2

(Fischer et al., 2016)

CycG^HR7 has embryonic/larval oenocyte phenotype, suppressible by wdb¹⁴/wdb^dw

(Fischer et al., 2016)

CycG^HR7 has phenotype, suppressible by Akt^UAS.Exel/Scer\GAL4^Lsp2.PH

(Fischer et al., 2015)

CycG^HR7 has embryonic/larval oenocyte | larval stage phenotype, suppressible by Akt^UAS.Exel/Scer\GAL4^Lsp2.PH

(Fischer et al., 2015)

CycG^HR7 has dorsal appendage | maternal effect phenotype, suppressible | maternal effect by mei-W68¹/mei-W68^k05603

(Nagel et al., 2012)

CycG^HR7 has dorsal appendage | maternal effect phenotype, suppressible | maternal effect by Df(1)BSC772/mei-41^RT1

(Nagel et al., 2012)

CycG^HR7 has dorsal appendage | maternal effect phenotype, suppressible | maternal effect by Df(2L)BSC258/lok^p6

(Nagel et al., 2012)

CycG^HR7 has dorsal appendage | maternal effect phenotype, suppressible | maternal effect | partially by Df(2L)BSC258/+

(Nagel et al., 2012)

Enhancer of

Statement

Reference

CycG^HR7/CycG^HR7 is an enhancer of wing vein | adult stage | ectopic phenotype of net¹

(Nagel et al., 2016)

CycG^HR7 is an enhancer of wing vein | adult stage phenotype of rho^ve-1

(Nagel et al., 2016)

CycG^HR7 is an enhancer of wing vein | adult stage phenotype of N^+tCos479

(Nagel et al., 2016)

CycG^HR7/CycG^HR7 is an enhancer of wing vein | adult stage phenotype of Delta^B2

(Nagel et al., 2016)

CycG^HR7/CycG^HR7 is an enhancer of wing vein | adult stage phenotype of H^P8

(Nagel et al., 2016)

CycG^HR7/CycG^HR7 is an enhancer | maternal effect of dorsal appendage | maternal effect phenotype of spn-B²/spn-B^BU

(Nagel et al., 2012)

Suppressor of

Statement

Reference

CycG^HR7 is a suppressor of wing vein | adult stage phenotype of vn¹

(Nagel et al., 2016)

Other

Statement

Reference

CycG^HR7, Xrcc2^CC has dorsal appendage phenotype

(Bayer et al., 2020)

CycG^HR7, Df(1)N-54l9/+ has wing blade phenotype

(Nagel et al., 2016)

CycG^HR7, Df(1)N-54l9/+ has wing vein | adult stage phenotype

(Nagel et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xrcc2^CC homozygous females in the heterozygous background of CycG^HR7 lay eggs with defective dorsal appendages (fused, narrowly spaced, branched or shortened) when compared to controls.

(Bayer et al., 2020)

The penetrance of the wing notching phenotype in CycG^HR7/+;Df(1)N-54l9/+ double heterozygote adult flies is about the same as in the Df(1)N-54l9/+ single heterozygote but is decreased in CycG^HR7/CycG^HR7;Df(1)N-54l9/+ flies.

CycG^eoC/+;Df(1)N-54l9/+ adult flies show significant L3 vein thickening compared to either CycG^HR7/CycG^HR7;Df(1)N-54l9/+ or CycG^HR7 mutant alone.

The vein knotting phenotype seen in N^+tCos479/+;CycG^HR7/+ mutants is enhanced in N^+tCos479/+;CycG^HR7/CycG^HR7 flies, more so in males than in females.

Su(H)^del47/+;CycG^HR7/CycG^HR7 display no phenotypic defects in adult wings.

The vein thickening phenotype of Dl^B2 heterozygotes is enhanced by combination with CycG^HR7. The severity of the phenotype differs with gender and vein position.

Proportion of flies with both L4 and L5 vein shortening phenotype was significantly increased in H^P8/+,CycG^HR7/CycG^HR7 mutants compared to H^P8/+ alone.

rho^ve-1,vn¹ double mutants almost completely lack veins in the adult wing. This phenotype was not changed by a further loss of CyG function in rho^ve-1,vn¹,CycG^HR7 triple mutants.

The vein loss phenotype of rho^ve-1 is slightly enhanced in rho^ve-1,CycG^HR7 double mutants but the effect is limited to L4 vein.

The loss of vein material in vn¹ is mildly suppressed in vn¹,CycG^HR7 double mutants.

The net¹ phenotype (ectopic veins, vein networking) can be enhanced by combination with CycG^HR7 but also by combination with mutant alleles of unrelated factors ru¹ or sp¹ demonstrating high susceptibility of the net¹ phenotype to perturbed genetic background.

(Nagel et al., 2016)

The reduced body size and weight of CycG^HR7 homozygote adults is fully rescued by combination with wdb mutant alleles in a heteroallelic state: the CycG^HR7,wdb^dw/CycG^HR7,wdb¹⁴ flies have normal both body size and weight.

The reduced body weight of CycG^HR7 larvae is fully rescued by ectopic expression of Akt1^{Scer\UAS.Exel} driven by Scer\GAL4^Lsp2.PH.

The lipid droplet accumulation in oenocytes of CycG^HR7 mutant larvae is highly suppressed by ectopic expression of Akt1^{Scer\UAS.Exel} under the control of the Scer\GAL4^Lsp2.PH driver.

(Fischer et al., 2015)

The ventralised eggshell phenotype seen in eggs derived from homozygous CycG^HR7 females is suppressed in more than 90% of eggs if the females are also carrying either mei-W68¹/mei-W68^k05603, lok^p6/Df(2L)BSC258 or mei-41^RT1/Df(1)BSC772.

The ventralised eggshell phenotype seen in eggs derived from homozygous CycG^HR7 females is partially suppressed by Df(2L)BSC258/+.

The ventralised eggshell phenotype of eggs derived from spn-B^BU CycG^HR7/spn-B² CycG^HR7 double mutant females is enhanced compared to the defects seen in eggs from spn-B^BU/spn-B² and CycG^HR7/CycG^HR7 single mutant females.

Eggs derived from Brca2^56E/Brca2^KO ; CycG^HR7/CycG^HR7 double mutant females have a mild eggshell patterning defect, which is not altered compared to the defect seen in eggs derived from Brca2^56E/Brca2^KO single mutant females.

(Nagel et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CycG^HR7 is rescued by CycG^hs.PF

(Fischer et al., 2015)

CycG^HR7 is rescued by CycG^UASp.cNa/Scer\GAL4^da.G32

(Nagel et al., 2012)

Partially rescued by

CycG^HR7 is partially rescued by Scer\GAL4^da.PU/CycG^UASp.cNa

(Fischer et al., 2015)

Comments

The reduced body weight of CycG^HR7 homozygote adults can be ameliorated by ubiquitous ectopic expression of CycG^{Scer\UAS.P\T.cNa} under the Scer\GAL4^da.PU driver, even though CycG^{Scer\UAS.P\T.cNa};Scer\GAL4^da.PU expression in a wild-type CycG background results in flies that are themselves slimmer than wild-type controls.

The reduced body weight of CycG^HR7 homozygote adults and larvae is fully reversed by expression of CycG^hs.PF even at ambient temperature.

The reduced egg laying rate of CycG^HR7 females can be fully rescued by ectopic expression of CycG^hs.PF at ambient temperature.

(Fischer et al., 2015)

Expression of CycG^{Scer\UAS.P\T.cNa} under the control of Scer\GAL4^da.G32 almost completely rescues the eggshell patterning defects of eggs derived from homozygous CycG^HR7 females.

(Nagel et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CycG^HR7

cycG^HR7

(Bayer et al., 2020, Fischer et al., 2016, Nagel et al., 2016, Nagel et al., 2012)

cycGHR7

(Fischer et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (5)

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