FlyBase Allele Report: Dmel\mei-W68[1]

General Information

Symbol

Dmel\mei-W68¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012191

Feature type

allele

Associated gene

Dmel\mei-W68

Associated Insertion(s)

?{}mei-W68¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Carpenter, 2003, McKim and Hayashi-Hagihara, 1998)

Progenitor genotype

Associated Insertion(s)

?{}mei-W68¹

Cytology

Description

Insertion of approximately 5kb into the second exon.

(McKim and Hayashi-Hagihara, 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:19,458,088..19,458,383

(Wei et al., 2019, Staeva-Vieira et al., 2003, McKim and Hayashi-Hagihara, 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle

abnormal meiotic cell cycle

(Bhagat et al., 2004, McKim et al., 1998)

abnormal meiotic cell cycle (with mei-W68^L1)

(Bhagat et al., 2004)

Phenotype Manifest In

early recombination nodule

(Carpenter, 2003)

late recombination nodule

(Carpenter, 2003)

Detailed Description

Statement

Reference

Homozygous females show elevated levels of chromosome nondisjunction of the X chromosome (34.7%).

X chromosome nondisjunction is increased to 51.9% in homozygous XXY females, with 95% of the events involving the two X chromosomes segregating from the Y.

(Wu et al., 2008)

The majority of mei-W68¹/mei-W68^k05603 eggs (94%) show a wild-type number of dorsal appendages.

(Klattenhoff et al., 2007)

mei-W68^L1/mei-W68¹ or mei-W68¹/mei-W68¹ female meioses exhibit crossover frequency reduction along chromosome 2, as compared to wild type.

(Bhagat et al., 2004)

Crossing over is reduced in mei-W68^L1/mei-W68¹ females. Homozygotes lack meiotic crossing over. Premeiotic exchanges occur in homozygous females. mei-W68¹ is recessive to wild type when nondisjunction frequencies are examined but has dominant effects on crossing over. Homozygous and mei-W68^L1/mei-W68¹ females show normal dynamics of germ-line mitotic divisions. The dynamics of entry into meiosis or exit of the losing pro-oocyte is also normal. The synaptonemal complex appears to be of normal morphology in homozygous females and appears to be between homologs. There are no late recombination nodules and early recombination nodules also appear to be missing. A novel structure ("noodle"), that resembles recombination nodules (RNs) in being adjacent to the central region of the synaptonemal complex, is seen. Noodles are smaller than RNs and are also less dense. The number of noodles increases at least up to the time cytoplasmic flow begins and they persist post-cytoplasmic flow.

(Carpenter, 2003)

Severe mutation.

(McKim et al., 1998)

Ultrastructural studies of pachytene reveal synaptonemal complex that is normal in structure and length and which undergoes the same changes in length as is observed in wild type as the cells progress through pachytene; chromocentral organization and chromatin condensation are also normal. However, no late recombination nodules were observed in the nine nuclei reconstructed which were between the developmental landmarks which demark their presence in wild type.

(Carpenter, 1997.5.12)

Metaphase arrest prevented.

(McKim et al., 1993)

Females homozygous for mei-W68 show a complete absence of meiotic recombination (Baker, unpublished data). A less severe allele (mei-W68^L1-Lindsley) reduces exchange to approximately 60% of control levels and also alters the distribution of residual exchanges. Analysis of mitotic chromosome behavior (Baker et al., 1978) suggests that the mei-W68⁺ gene product is also required in mitotic cells.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mei-W68¹ is a suppressor of abnormal DNA repair | oogenesis phenotype of p53^A2.3

(Chakravarti et al., 2022)

mei-W68¹ is a suppressor of abnormal DNA repair | oogenesis phenotype of p53^B41.5

(Chakravarti et al., 2022)

mei-W68¹ is a suppressor of female sterile phenotype of okr^AA/okr^RU, p53^unspecified

(Lu et al., 2010)

mei-W68¹ is a suppressor of female sterile phenotype of spn-B^BU

(Liu et al., 2002)

mei-W68¹ is a suppressor of female sterile | recessive phenotype of spn-B^BU

(Liu et al., 2000)

Other

Statement

Reference

Df(3R)3450/yem¹, mei-W68¹ has abnormal meiotic cell cycle | female phenotype

(Meyer et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

mei-W68¹ is a suppressor of ovariole phenotype of p53^A2.3

(Chakravarti et al., 2022)

mei-W68¹ is a suppressor of ovariole phenotype of p53^B41.5

(Chakravarti et al., 2022)

mei-W68¹/mei-W68^k05603 is a suppressor | maternal effect of dorsal appendage | maternal effect phenotype of CycG^HR7

(Nagel et al., 2012)

mei-W68¹ is a suppressor of nurse cell phenotype of okr^AA/okr^RU, p53^unspecified

(Lu et al., 2010)

mei-W68¹/mei-W68¹ is a suppressor of egg chorion phenotype of okr^AA/okr^RU

(Barbosa et al., 2007)

mei-W68¹/mei-W68¹ is a suppressor of egg chorion phenotype of spn-A^093A/spn-A¹

(Barbosa et al., 2007)

mei-W68¹/mei-W68¹ is a suppressor of egg chorion | germline clone phenotype of pds5¹

(Barbosa et al., 2007)

mei-W68¹/mei-W68¹ is a suppressor of egg chorion | germline clone phenotype of pds5²

(Barbosa et al., 2007)

mei-W68¹/mei-W68¹ is a suppressor of egg chorion | germline clone phenotype of pds5³

(Barbosa et al., 2007)

mei-W68¹/mei-W68¹ is a suppressor of egg chorion | germline clone phenotype of pds5⁶

(Barbosa et al., 2007)

mei-W68¹ is a suppressor of egg chamber phenotype of mio²

(Iida and Lilly, 2004)

mei-W68[+]/mei-W68¹ is a suppressor of nurse cell phenotype of mio²

(Iida and Lilly, 2004)

mei-W68¹/mei-W68¹ is a suppressor of nurse cell phenotype of mio²

(Iida and Lilly, 2004)

mei-W68¹/mei-W68¹ is a suppressor of oocyte phenotype of mio²

(Iida and Lilly, 2004)

mei-W68[+]/mei-W68¹ is a suppressor of oocyte phenotype of mio²

(Iida and Lilly, 2004)

mei-W68[+]/mei-W68¹ is a suppressor | partially of nurse cell phenotype of mio²/mio¹

(Iida and Lilly, 2004)

mei-W68[+]/mei-W68¹ is a suppressor | partially of oocyte phenotype of mio²/mio¹

(Iida and Lilly, 2004)

mei-W68¹ is a suppressor of egg chorion | dorsal phenotype of spn-A^093A/Df(3R)X3F

(Staeva-Vieira et al., 2003)

mei-W68¹ is a suppressor of dorsal appendage phenotype of spn-A^093A/Df(3R)X3F

(Staeva-Vieira et al., 2003)

NOT Suppressor of

Statement

Reference

mei-W68¹/mei-W68^k05603 is a non-suppressor of dorsal appendage phenotype of armi^72.1/armi¹

(Klattenhoff et al., 2007)

mei-W68¹ is a non-suppressor of karyosome phenotype of Src64B^Δ17

(Djagaeva et al., 2005)

Other

Statement

Reference

Df(2R)LL5, mei-41^D3, mei-W68¹ has oocyte nucleus & meiotic cell cycle phenotype

(McKim et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The ventralised eggshell phenotype seen in eggs derived from homozygous CycG^HR7 females is suppressed in more than 90% of eggs if the females are also carrying mei-W68¹/mei-W68^k05603.

(Nagel et al., 2012)

Single-gene mutants show 15 nurse cell nuclei per egg chamber, but p53^unspecified, okr^AA/okr^RU ovaries exhibit a broad distribution, ranging from 9 to 40 nuclei per egg chamber, which is restored to normal in mei-W68¹, okr^AA/okr^RU, p53^unspecified animals.

While p53^unspecified, okr^AA/okr^RU double mutants are sterile, fertility is restored in mei-W68¹, okr^AA/okr^RU, p53^unspecified triple mutant females.

(Lu et al., 2010)

mei-W68¹ ; yemα¹/Df(3R)3450 oocytes undergo precocious anaphase I (as occurs in mei-W68¹ single mutants), but meiosis II spindles are rarely observed in the double mutants (in contrast to mei-W68¹ single mutants where a greater proportion of the oocytes reach meiosis II).

(Meyer et al., 2010)

The fused dorsal appendage phenotype of armi^72.1/armi¹ eggs is not suppressed in mei-W68¹/mei-W68^k05603; armi^72.1/armi¹ eggs.

(Klattenhoff et al., 2007)

Transformation of oocytes to nurse cells in mio² homozygous females is partially suppressed by mei-W68¹/+ or mei-W68¹/mei-W68¹. The block in oogenesis at around stage 5 seen in mio² homozygous females is also partially suppressed by mei-W68¹, producing egg chambers that often undergo vitellogenesis and develop to the late stages of oogenesis. Transformation of oocytes to nurse cells in mio²/mio¹ females is suppressed by mei-W68¹/+.

(Iida and Lilly, 2004)

Has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males.

(McKee et al., 2000)

In mei-41^D3; mei-W68¹/Df(2R)LL5 oocytes most nuclei exhibit either disorganised spindles and multiple chromatin masses or premature anaphases.

In mei-41^D3; mei-W68¹/Df(2R)LL5 oocyte nuclei most nuclei are either exhibit disorganised spindles and multiple chromatin masses or premature anaphases.

(McKim et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mei-W68^W3

(Shulman et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4932

y¹/Dp(1;Y)y⁺; mei-W68¹/CyO

Kyoto

107952

y¹/Dp(1;Y)y⁺; mei-W68¹/CyO

Notes on Origin

Discoverer

Comments

No gene conversion or intragenic crossovers are observed in an assay of intragenic recombination at ry. The frequency of crossing-over on the entire X and second chromosome is much reduced: isolated recombination events suggest that the recombination taken place is in fact a mitotic and not a meiotic event. Examination of oocytes to study meiotic recombination events in sister chromatids reveals there are no cross over events. Double stranded breaks are not left unrepaired. Synaptonemal complex formation is not disrupted, meiotic progression is normal.

(McKim et al., 1998)

Complements: par-1^k06323.

(Shulman et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mei-w68¹

(Roelens et al., 2017)

mei-W68¹

(Jansen et al., 2024, Vallés et al., 2024, Chakravarti et al., 2022, Wei et al., 2019, Rossi et al., 2017, Nagel et al., 2012, Meyer et al., 2010, Klattenhoff et al., 2009, Wu et al., 2008, Barbosa et al., 2007, Klattenhoff et al., 2007, McCaffrey et al., 2006, Bhagat et al., 2004, Jang et al., 2003)

mei-W68^Baker

spo11^-

(Lu et al., 2010)

Name Synonyms

Secondary FlyBase IDs

FBal0086409

References (32)

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