FlyBase Allele Report: Dmel\mio[2]

General Information

Symbol

Dmel\mio²

Species

D. melanogaster

Name

FlyBase ID

FBal0158954

Feature type

allele

Associated gene

Dmel\mio

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Iida and Lilly, 2004)

Mutagen

ethyl methanesulfonate

(Iida and Lilly, 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: W817term.

(Iida and Lilly, 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:2,220,195..2,220,195 [+]

Nucleotide change:

G2220195A

Amino acid change:

W817term | mio-PA

Reported amino acid change:

W817term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Iida and Lilly, 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | germline clone

(Wei et al., 2014)

decreased body size | larval stage

(Jouandin et al., 2022)

female sterile (with Df(2L)yan-J2)

(Iida and Lilly, 2004)

female sterile (with mio¹)

(Iida and Lilly, 2004)

female sterile | recessive

(Iida and Lilly, 2004)

Phenotype Manifest In

autolysosome (with Df(2L)Exel6007)

(Wei et al., 2014)

centriole

(Senger et al., 2011)

egg chamber

(Iida and Lilly, 2004)

egg chamber (with Df(2L)Exel6007)

(Wei et al., 2014)

egg chamber (with mio¹)

(Wei et al., 2014, Senger et al., 2011)

egg chamber | germline clone

(Wei et al., 2014)

microtubule

(Senger et al., 2011)

nurse cell

(Iida and Lilly, 2004)

nurse cell (with Df(2L)yan-J2)

(Iida and Lilly, 2004)

nurse cell (with mio¹)

(Iida and Lilly, 2004)

oocyte

(Senger et al., 2011, Iida and Lilly, 2004)

oocyte (with Df(2L)yan-J2)

(Iida and Lilly, 2004)

oocyte (with mio¹)

(Iida and Lilly, 2004)

ovary

(Cai et al., 2016)

Detailed Description

Statement

Reference

mio²/mio² ovaries show accumulation of acidic puncta.

(Cai et al., 2016)

Egg chambers containing germline clones of mio² are dramatically smaller and have nurse cells with decreased ploidy values relative to adjacent wild type egg chambers. Clones in somatic cells, including the fat body and somatic cells of the ovary, show only a small decrease, if any, in cell size and ploidy.

Ovarian cysts, but not fat bodies, from mio²/Df(2L)Exel6007 animals accumulate autolysosomes.

Egg chambers from mio¹/mio² females often develop without an oocyte and cease to develop beyond stage 5 or 6 of oogenesis.

(Wei et al., 2014)

In mio¹/mio² mutants, the vast majority of egg chambers arrest prior to stage 5 of oogenesis, well before the start of vitellogenesis in stage 7.

In homozygous mio² egg chambers, the centrioles fail to migrate to the posterior of the oocyte. In the mutant egg chambers, the distribution of microtubules is frequently disorganised.

(Senger et al., 2011)

mio² mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38^oC, but show a reduction in SSA frequency when assayed at 32^oC.

(Wei and Rong, 2007)

90% of egg chambers in mio¹ homozygotes have 16-polyploid nurse cells and no oocyte. These egg chambers rarely develop beyond stage 5 of oogenesis. The severity of this phenotype not significantly different in mio²/Df(2L)yan-J2, suggesting that this allele is a genetically null.

(Iida and Lilly, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

mio²/mio¹ has egg chamber phenotype, suppressible by Scer\GAL4^nanos.PG/Nprl2^RNAi.UAS.V20

(Wei et al., 2014)

mio²/mio¹ has egg chamber phenotype, suppressible by Scer\GAL4^nanos.PG/Nprl3^RNAi.UAS.V20

(Wei et al., 2014)

mio²/mio¹ has egg chamber phenotype, suppressible by Scer\GAL4^nanos.PG/Tsc1^GL00012

(Wei et al., 2014)

mio²/mio¹ has egg chamber phenotype, suppressible | partially by Nup44A[+]/Nup44A^EP2417

(Senger et al., 2011)

mio²/mio¹ has egg chamber phenotype, suppressible by Df(2R)ED1735/+

(Senger et al., 2011)

mio²/mio¹ has egg chamber phenotype, suppressible by Nup44A^Δ15/Nup44A[+]

(Senger et al., 2011)

mio² has egg chamber phenotype, suppressible by mei-P22^P22

(Iida and Lilly, 2004)

mio² has egg chamber phenotype, suppressible by mei-W68¹

(Iida and Lilly, 2004)

mio² has nurse cell phenotype, suppressible by mei-P22[+]/mei-P22^P22

(Iida and Lilly, 2004)

mio² has nurse cell phenotype, suppressible by mei-P22^P22/mei-P22^P22

(Iida and Lilly, 2004)

mio² has nurse cell phenotype, suppressible by mei-W68[+]/mei-W68¹

(Iida and Lilly, 2004)

mio² has nurse cell phenotype, suppressible by mei-W68¹/mei-W68¹

(Iida and Lilly, 2004)

mio² has nurse cell phenotype, suppressible by mei-W68[+]/mei-W68^k05603

(Iida and Lilly, 2004)

mio² has oocyte phenotype, suppressible by mei-P22[+]/mei-P22^P22

(Iida and Lilly, 2004)

mio² has oocyte phenotype, suppressible by mei-P22^P22/mei-P22^P22

(Iida and Lilly, 2004)

mio² has oocyte phenotype, suppressible by mei-W68¹/mei-W68¹

(Iida and Lilly, 2004)

mio² has oocyte phenotype, suppressible by mei-W68[+]/mei-W68^k05603

(Iida and Lilly, 2004)

mio² has oocyte phenotype, suppressible by mei-W68[+]/mei-W68¹

(Iida and Lilly, 2004)

mio²/mio¹ has nurse cell phenotype, suppressible | partially by mei-W68[+]/mei-W68¹

(Iida and Lilly, 2004)

mio²/mio¹ has nurse cell phenotype, suppressible | partially by mei-W68[+]/mei-W68^k05603

(Iida and Lilly, 2004)

mio²/mio¹ has oocyte phenotype, suppressible | partially by mei-W68[+]/mei-W68^k05603

(Iida and Lilly, 2004)

mio²/mio¹ has oocyte phenotype, suppressible | partially by mei-W68[+]/mei-W68¹

(Iida and Lilly, 2004)

NOT suppressed by

Statement

Reference

mio² has ovary phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl2^RNAi.UAS.V20

(Cai et al., 2016)

mio² has ovary phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl3^RNAi.UAS.V20

(Cai et al., 2016)

mio² has nurse cell phenotype, non-suppressible by mei-41[+]/mei-41^29D

(Iida and Lilly, 2004)

mio² has nurse cell phenotype, non-suppressible by mei-41^29D/mei-41^29D

(Iida and Lilly, 2004)

mio² has oocyte phenotype, non-suppressible by mei-41[+]/mei-41^29D

(Iida and Lilly, 2004)

mio² has oocyte phenotype, non-suppressible by mei-41^29D/mei-41^29D

(Iida and Lilly, 2004)

Enhancer of

Statement

Reference

mio² is an enhancer of egg chamber phenotype of Nup44A^Δ15

(Senger et al., 2011)

Other

Statement

Reference

egl¹, mio² has cystoblast phenotype

(Iida and Lilly, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nprl2^{dsRNA.shRNA.Scer\UAS} or Nprl3^{dsRNA.shRNA.Scer\UAS} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress accumulation of acidic puncta in mio²/mio² ovaries.

(Cai et al., 2016)

Nup44A^EP2417/+ partially suppresses the mio¹/mio² oogenesis phenotype. In contrast to mio¹/mio² single mutants, egg chambers from Nup44A^EP2417/+, mio¹/mio² females frequently progress through vitellogenesis to produce mature eggs. Moreover, a small percentage of the eggs laid by Nup44A^EP2417/+, mio¹/mio² females hatch and develop into viable adults.

The percentage of mio¹/mio² mutant egg chambers with an oocyte increases 6-fold in a Df(2R)ED1735/+ genetic background.

The percentage of mio¹/mio² mutant egg chambers with an oocyte increases 6-fold in a Nup44A^Δ15/+ genetic background.

Ovaries from mio², Nup44A^Δ15 double mutant females closely resemble those from Nup44A^Δ15 single mutants, with 80% or egg chambers containing an oocyte and 20% containing 16 nurse cells and no obvious oocyte. However, ovaries from mio², Nup44A^Δ15 double homozygotes have fewer older egg chambers than Nup44A^Δ15 single mutants.

(Senger et al., 2011)

Transformation of oocytes to nurse cells in mio² homozygous females is partially suppressed by mei-W68¹/+, mei-W68¹/mei-W68¹, mei-P22^P22/+, mei-P22^P22/mei-P22^P22 or mei-W68^k05603/+, but not by mei-41^29D/+ or mei-41^29D/mei-41^29D. The block in oogenesis at around stage 5 seen in mio² homozygous females is also partially suppressed by mei-W68¹ or mei-P22^P22, producing egg chambers that often undergo vitellogenesis and develop to the late stages of oogenesis. Transformation of oocytes to nurse cells in mio²/mio¹ females is suppressed by mei-W68¹/+ or mei-W68^k05603/+. mio²; egl¹ double homozygotes have a phenotype significantly stronger than either single mutant: All cystoblasts lack obvious synaptonemal complexes.

(Iida and Lilly, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mio² is rescued by Scer\GAL4^{VP16.nanos.UTR}/mio^UASp.cIa

(Iida and Lilly, 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Mulligan

(Iida and Lilly, 2004)

Induced with: stil³. Separable from: stil³.

(Iida and Lilly, 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mio²

(Jouandin et al., 2022, Wei et al., 2019, Cai et al., 2016, Wei et al., 2014, Senger et al., 2011, Wei and Rong, 2007)

Name Synonyms

Secondary FlyBase IDs

References (7)

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