Wings of homozygous pk30, rhove-1 and vn1 triple mutant flies lack veins L2-5. Wing anterior hairs of these mutants consistently have a posterior component to their polarity and posterior hairs have an anterior component to their polarity.
Introduction of one copy of kst1 into a homozygous rhove-1 background completely suppresses the wing vein defect for L2-L4 and partially suppresses the defects in L5. A kst2 heterozygous background results in a similar, but milder suppression.
Expression of UbqndsRNA.Ex2.Scer\UAS by Scer\GAL4e22c in a heterozygous rhove-1 background leads to a normal anterior cross vein and partially restored posterior cross vein and L5 vein, while the distal portion of L4 and L3-4 M veins corresponding to the posterior portion of the wing remains weakened, compared with UbqndsRNA.Ex2.Scer\UAS; Scer\GAL4e22c single mutants.
Df(2R)ED3921; rhove-1 flies show an enhancement of L5 vein loss compared to rhove-1 flies single mutants and partial loss of L2, a phenotype not seen in rhove-1 flies. There is no significant enhancement of L4 and L3 loss.
rhove-1 vn1 double homozygotes lack wing veins. This phenotype is partially suppressed by ash2S112411 /ash2S112411. The degree of suppression varies from development of L2 only to almost complete rescue, albeit with some abnormalities such as extra crossvein material or proximal fusions between L2 and L3 or L4 and L5. In 25% of cases, hollow, tube like wings are formed, probably due to detachment of dorsal and ventral layers.
brm2 enhances the loss of wing vein L5 seen in rhove-1 homozygotes. Snr1E1/Snr1R3 suppresses the loss of wing vein L5 seen in rhove-1 homozygotes and rhove-1 suppresses the Snr1E1/Snr1R3 extra wing vein phenotype. Snr1E1 suppresses the shortened L5 wing vein that results from the interaction between rhove-1 and brm2.
rhove-1 vn1 double mutant flies lack all the longitudinal veins and crossveins of the wing blade. Dorsal and ventral implants of rhove-1 vn1 imaginal disc tissue into wild-type imaginal discs fail to differentiate veins.
vn1 rhove-1 double homozygotes lack all longitudinal wing veins. Vein tissue differentiates in vn1 rhove-1 double homozygotes when dppScer\UAS.cSa is expressed in these flies using Scer\GAL4C-580. rhove-1 suppresses the differentiation of thicker veins seen in tkv1/tkv8 flies, and results in fewer veins in combination with either dpps4/dpps8 or tkv1/tkv8.
Moderate expression of Zzzz\lefScer\UAS.cGa under the control of Scer\GAL4Bx-MS1096 in rhove-1 heterozygotes results in wing vein loss, while moderate Zzzz\lefScer\UAS.cGa expression (with Scer\GAL4Bx-MS1096) alone doesn't have this phenotype.