FlyBase Allele Report: Dmel\spn-B[2]

General Information

Symbol

Dmel\spn-B²

Species

D. melanogaster

Name

FlyBase ID

FBal0016034

Feature type

allele

Associated gene

Dmel\spn-B

Associated Insertion(s)

Carried in Construct

Also Known As

spn-B¹⁵³, spnB¹⁵³

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Gonzalez-Reyes et al., 1997)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: G102R.

(Ghabrial et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:14,328,635..14,328,635 [+]

Nucleotide change:

G14328635A

Amino acid change:

G102R | spn-B-PA

Comment:

site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change

(Ghabrial et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Ghabrial et al., 1998)

Phenotype Manifest In

dorsal appendage

(Ghabrial et al., 1998)

dorsal appendage (with spn-B^BU)

(Bayer et al., 2020)

dorsal appendage | maternal effect (with spn-B^BU)

(Nagel et al., 2012)

egg chorion | maternal effect (with spn-B^BU)

(Ghabrial and Schupbach, 1999)

karyosome

(Ghabrial and Schupbach, 1999)

Detailed Description

Statement

Reference

spn-B²/spn-B^BU but not spn-B²/+ females lay eggs with defective dorsal appendages (fused, narrowly spaced or missing) when compared to controls.

(Bayer et al., 2020)

spn-B²/spn-B^BU females produce ventralised eggs that have fused dorsal appendages.

(Nagel et al., 2012)

Homozygous and hemizygous spn-B²/Df(3R)trxE12 females produce eggs with a range of eggshell phenotypes; from resembling wild-type with two normal dorsal appendages, having two dorsal appendages fused at the base, having a single dorsal appendage to having little or no dorsal appendage material. Hemizygotes are viable. Eggs derived from fs(1)K10^unspecified; spn-B²/spn-B¹ females show a broad spectrum of phenotypes ranging from completely dorsalised to completely ventralised. spn-B¹/spn-B² and spn-B²/spn-B^BU larvae show normal sensitivity to methyl methanesulfonate. Recombination frequency is 10-25% of normal levels in spn-B²/spn-B^BU females, and X chromosome non-disjunction is increased 100-fold compared to wild-type.

(Ghabrial et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

spn-B²/spn-B^BU has dorsal appendage phenotype, enhanceable by Xrcc2^CC/Xrcc2^CC

(Bayer et al., 2020)

spn-B²/spn-B^BU has dorsal appendage | maternal effect phenotype, enhanceable | maternal effect by CycG^HR7/CycG^HR7

(Nagel et al., 2012)

Suppressed by

Statement

Reference

spn-B²/spn-B^BU has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-W68^unspecified/Df(2R)LL5

(Ghabrial and Schupbach, 1999)

spn-B² has karyosome phenotype, suppressible by mei-W68^unspecified

(Ghabrial and Schupbach, 1999)

Enhancer of

Statement

Reference

spn-B²/spn-B^BU is an enhancer | maternal effect of dorsal appendage | maternal effect phenotype of CycG^HR7

(Nagel et al., 2012)

Other

Statement

Reference

Xrcc2^CC, spn-B² has dorsal appendage phenotype

(Bayer et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xrcc2^CC homozygous females in the heterozygous background of spn-B² lay eggs with defective dorsal appendages (fused, narrowly spaced, branched or shortened) when compared to controls.

(Bayer et al., 2020)

The ventralised eggshell phenotype of eggs derived from spn-B^BU CycG^HR7/spn-B² CycG^HR7 double mutant females is enhanced compared to the defects seen in eggs from spn-B^BU/spn-B² and CycG^HR7/CycG^HR7 single mutant females.

(Nagel et al., 2012)

The eggshell patterning defects of eggs derived from spn-B²/spn-B^BU females are suppressed if the females also carry mei-W68^unspecified/Df(2R)LL5.

(Ghabrial and Schupbach, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

605308

ru¹ Diap1^th-1 st¹ kni^ri-1 rn^roe-1 p^p spn-B²/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

spn-B¹⁵³

(Bayer et al., 2020, Nagel et al., 2012, Findley et al., 2003, Abdu et al., 2002, Ghabrial and Schupbach, 1999, Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987)

spn-B²

spnB¹⁵³

(Li et al., 2014, Ghabrial et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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