Response to sound, measured via compound action potentials in the antennal nerve, is disrupted in flies in which a subset of the Johnston's organ neurons has been eliminated by via Rcom\RA[Scer\UAS.cSa] driven by Scer\GAL4JO15 + Scer\GAL4ph-p-NP1046.
Ablation of Johnston's organ "C" and "E" neurons via Scer\GAL4NP6250-mediated expression of Rcom\RAScer\UAS.cSa eliminates the wind-induced suppression of locomotion response, whereas basal locomotor activity, phototaxis behaviour, and hearing are unaffected.
Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4elav.PU results in ablation of neurons in embryos, but longitudinal glia divisions that occur before axonal contact (longitudinal glioblast up to the 4-longitudinal glia stage) are unaffected.
Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4Mz19 results in ablation of all Scer\GAL4Mz19-expressing projection neurons (including those innervating glomeruli DA1 and VA1d) and the absence of their dendrites from the corresponding glomeruli.
Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4Or88a.cWa or Scer\GAL4Or47b.7.467 ablates the cell bodies (in the antenna) and axons (in the antennal lobe) of Or88a/Or47b-expressing olfactory receptor neurons (ORNs), resulting in a significant reduction in the size of their respective glomeruli (VA1d and VA1lm). After ablation of Or88a/Or47b-positive ORNs, the associated Scer\GAL4Mz19-expressing projection neuron (PN) dendrites remain confined to their target glomeruli for up to 30 days - that is, ablation of a single ORN class does not alter the dendritic innervation pattern of PNs normally projecting to the same or a neighbouring glomerulus.
Induction of expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4Mz19 upon eclosion leads to death of Scer\GAL4Mz19-expressing projection neurons (PNs). PN death does not appear to alter glomerular size, neither does it affect innervation of the VA1d glomerulus by Or88a-positive olfactory receptor neurons or ORN axons that project to Scer\GAL4Mz19-negative glomeruli.
Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4how-24B results in a failure of germband retraction and the majority of the mesoderm often appears to be absent at 29oC.
Scer\GAL4sd-SG29.1-mediated expression for 1 hour at 37oC causes cell death in the wing pouch.