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General Information
Symbol
Rcom\RTA.UAS
Species
R. communis
Name
Saccharomyces cerevisiae UAS construct a of Smith
FlyBase ID
FBal0239059
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Ricin, UAS-ricinA, UASricin, UAS-Ricin A, UFR1.1, UAS ricin
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The FRT cassette has been excised, allowing the UAS regulatory sequences to drive expression of RT-A.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Response to sound, measured via compound action potentials in the antennal nerve, is disrupted in flies in which a subset of the Johnston's organ neurons has been eliminated by via Rcom\RA[Scer\UAS.cSa] driven by Scer\GAL4JO15 + Scer\GAL4ph-p-NP1046.

Embryos expressing Rcom\RAScer\UAS.cSa in the mesoderm under the control of Scer\GAL4bap.3 show only residual migration of the endoderm.

Embryos expressing Rcom\RAScer\UAS.cSa in the mesoderm under the control of Scer\GAL4miple2.PW have no visible endoderm at stage 13 and the visceral mesoderm appears disorganised.

Ablation of Johnston's organ "C" and "E" neurons via Scer\GAL4NP6250-mediated expression of Rcom\RAScer\UAS.cSa eliminates the wind-induced suppression of locomotion response, whereas basal locomotor activity, phototaxis behaviour, and hearing are unaffected.

Embryos expressing Rcom\RAScer\UAS.cSa in the haemocytes under the control of Scer\GAL4srp.Hemo display ventral nerve cord condensation defects.

Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4elav.PU results in ablation of neurons in embryos, but longitudinal glia divisions that occur before axonal contact (longitudinal glioblast up to the 4-longitudinal glia stage) are unaffected.

Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4Mz19 results in ablation of all Scer\GAL4Mz19-expressing projection neurons (including those innervating glomeruli DA1 and VA1d) and the absence of their dendrites from the corresponding glomeruli.

Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4Or88a.cWa or Scer\GAL4Or47b.7.467 ablates the cell bodies (in the antenna) and axons (in the antennal lobe) of Or88a/Or47b-expressing olfactory receptor neurons (ORNs), resulting in a significant reduction in the size of their respective glomeruli (VA1d and VA1lm). After ablation of Or88a/Or47b-positive ORNs, the associated Scer\GAL4Mz19-expressing projection neuron (PN) dendrites remain confined to their target glomeruli for up to 30 days - that is, ablation of a single ORN class does not alter the dendritic innervation pattern of PNs normally projecting to the same or a neighbouring glomerulus.

Induction of expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4Mz19 upon eclosion leads to death of Scer\GAL4Mz19-expressing projection neurons (PNs). PN death does not appear to alter glomerular size, neither does it affect innervation of the VA1d glomerulus by Or88a-positive olfactory receptor neurons or ORN axons that project to Scer\GAL4Mz19-negative glomeruli.

Flies expressing Rcom\RAScer\UAS.cSa under the control of Scer\GAL4cad-em459 lack all terminal structures. The genital discs do not proliferate in third instar larvae.

Expression of Rcom\RAScer\UAS.cSa under the control of Scer\GAL4how-24B results in a failure of germband retraction and the majority of the mesoderm often appears to be absent at 29oC.

Scer\GAL4sd-SG29.1-mediated expression for 1 hour at 37oC causes cell death in the wing pouch.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The segregation of the dendritic field of the Scer\GAL4Mz19-positive projection neurons to nonadjacent regions in the antennal lobe that is seen in animals expressing capsScer\UAS.cSa under the control of Scer\GAL4Mz19 still occurs if all the olfactory receptor neurons have been ablated (using Scer\FLP1ey.PN to generate Rcom\RAScer\UAS.cSa from Rcom\RAScer\UAS.Scer\FRT and using Scer\GAL4peb-GAL4 to then express the Rcom\RAScer\UAS.cSa produced in the olfactory receptor neurons).

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Exists in clones, not reported as being germ-line transmitted.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
Reported As
Symbol Synonym
RAUAS.cBa
RAUAS.cDa
RAUAS.cSa
Rcom\RAScer\UAS.cBa
Rcom\RAScer\UAS.cSa
Rcom\RAUAS.cSa
Rcom\RTA.UAS
Rcom\RTUAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Brand et al
Saccharomyces cerevisiae UAS construct a of Smith
Secondary FlyBase IDs
  • FBal0047102
  • FBal0058384
References (39)