Amino acid replacement: S52D.
UASp regulatory sequences drive expression of a full-length sn cDNA (RH62992), in which codon 52 has been mutagenised to encode a phosphomimetic D instead of a S, with EGFP fused to the N-terminus.
snS52D.UASp.EGFP/Scer\GAL4srp.Hemo rescues Df(1)C128
Scer\GAL4srp.Hemo-driven expression of snS52D.Scer\UAS.P\T.T:Avic\GFP-EGFP restores the polarity and the dynamic leading edge cortex that is normally lacking in plasmatocytes in Df(1)C128 embryos. Normal developmental migration of plasmatocytes is also restored.