Expression of dlScer\UAS.cMa in circulating haemocytes, lymph glands, and epidermis, but not in the fat body, under the control of Scer\GAL4e33C, restores normal haemocyte numbers in Dif1 dl1 double mutants. The rescued blood cells are indistinguishable in morphology from wild-type uninfected haemocytes. In addition, the rescued animals have do not have any microbes in their haemolymph and 66% survive to adult stages.
Expression of dlScer\UAS.cMa in circulating haemocytes, but not in other immune-responsive tissues, under the control of Scer\GAL4He.PZ, results in an absence of microbes from the haemolymph, with approximately 45% of dlScer\UAS.cMa expressing Dif1 dl1 double mutants surviving till adulthood.
Expression of dlScer\UAS.cMa in circulating haemocytes and lymph gland cells, under the control of Scer\GAL4srp.Hemo, increases blood cell number to approximately 90% of wild-type in Dif1 dl1 double mutants and enables approximately 50% survival till adulthood. The majority of these larvae have no microbes in their haemolymph.