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General Information
Symbol
Dmel\Pvrλ.UASp.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0127283
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-λpvr, λ-PVR, UAS-Pvract
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of an activated form of Pvr in which the Pvr extracellular ligand-binding domain has been replaced by the dimerization domain of the bacteriophage λ cI repressor (to induce constitutive dimerization of the encoded protein). A Tag:MYC epitope has been inserted into the BglII site at amino acid residue 785, N-terminal of the transmembrane domain.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larvae expressing Pvrλ.UASp.Tag:MYC under the control of Scer\GAL4dpp.blk1 show bigger wing discs.

Scer\GAL4He.PZ-mediated expression of Pvrλ.Scer\UAS.T:Hsap\MYC increases hemocyte counts in larvae.

Scer\GAL4Bx-MS1096-mediated expression of Pvrλ.Scer\UAS.T:Hsap\MYC induces hypertrophic tumor-like structures.

Expression of Pvrλ.UASp.Tag:MYC in larvae under the control of Scer\GAL4repo results in overmigration of glial cells along the Bolwig nerve compared to controls.

Migration of border cells is severely impaired in Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL4slbo.2.6 animals.

Hemocytes in Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL4srp.Hemo late embryos do not exhibit abnormal aggregation.

Hemocytes in Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL4srp.Hemo late stage embryos aggregate abnormally, but a low percentage have a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band. These animals are viable.

The polarity of the epithelia in Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL469B embryos is normal. The cuticles made by these embryos are normal apart from holes at the termini. The organization of filamentous actin in the eye discs of Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL4GMR.PF larvae is normal.

When Pvrλ.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4slbo.2.6 in the follicle cells, an increase is seen in the accumulation of F-actin. These cells also show a disruption of the normal cell shape.

When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation and lamellocyte numbers are increased, and crystal cells are unaffected. Melanotic masses form.

When clones of Pvrλ.Scer\UAS.T:Hsap\MYC expressing cells (driven by Scer\GAL4Act5C.PI) are made in the follicle cells, massive F-actin accumulation, actin rich extensions and changes in cell shape are seen. When Pvrλ.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4slbo.2.6, causes disruption in centripetal cell morphology and abnormal actin accumulation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Phenotype Manifest In
Suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

When Pvrλ.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4slbo.2.6 in a homozygous Cortactinunspecified background, a small but significant suppression is seen in the F-Actin phenotype.

When mbcD11.2 mutant somatic clones are made in border cells in addition to Pvrλ.Scer\UAS.T:Hsap\MYC, driven by Scer\GAL4slbo.2.6, a strong attenuation of the Pvrλ.Scer\UAS.T:Hsap\MYC phenotype is seen.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL4srp.Hemo rescues the macrophage aggregation phenotype of stage 16 Pvr1 homozygous embryos. However, these embryos retain a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Pvrλ.Scer\UAS.T:Hsap\MYC
Pvrλ.UASp.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (18)