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General Information
Symbol
Dmel\Pvr1
Species
D. melanogaster
Name
FlyBase ID
FBal0197800
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G8223121A

Amino acid change:

W1087term | Pvr-PA; W1087term | Pvr-PB; W1087term | Pvr-PD; W1081term | Pvr-PE; W1046term | Pvr-PG; W1052term | Pvr-PH; W1155term | Pvr-PI; W1155term | Pvr-PJ; W1078term | Pvr-PK; W1087term | Pvr-PL; W1146term | Pvr-PM; W1077term | Pvr-PN; W1077term | Pvr-PO

Reported amino acid change:

W1087term

Comment:

Reported as a change in Trp 1087 from TGG to TGA.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W1087term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Pvr1 mutants exhibit a severe hemocyte migration defect in which the cells are unable to migrate from their origin in the head and undergo apoptosis.

By stage 12, Pvr1 homozygous embryos contain large clusters of macrophages.

Macrophages in stage 16 Pvr1 homozygous embryos contain multiple engulfed apoptotic hemocytes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Border cells which are part of somatic clones of Pvr1; Egfrf2 double homozygous cells initiate migration, but fail to reach the oocyte.

Ras85DV12.Scer\UAS; Scer\GAL4srp.Hemo suppresses the macrophage aggregation phenotype of stage 16 Pvr1 homozygous embryos. However, these embryos have a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band.

Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX; Scer\GAL4srp.Hemo suppresses the reduction in hemocytes seen at stage 12 in Pvr1 homozygous embryos, but fails to suppress the reduction in hemocyte numbers at stage 16 and only mildly suppresses the macrophage aggregation phenotype.

Xenogenetic Interactions
Statement
Reference

The Pvr1 hemocyte migration phenotype can be suppressed through expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4srp.Hemo. The rescued hemocytes are not only unable to inflitrate the germ band, but also fail to migrate posteriorly from the head along the central nervous system and the dorsal vessel.

Pvr1 BacA\p35Scer\UAS.cHa (Scer\GAL4Pxn.PS) mutants show a robust wild-type response to laser ablation, indicating that hemocyte chemotaxis towards wounds is Pvr independent.

Clustering of macrophages, the presence of engulfed apoptotic hemocytes in macrophages and reduction in hemocyte numbers in Pvr1 embryos are all eliminated by BacA\p35Scer\UAS.cHa; Scer\GAL4srp.Hemo. However, hemocyte distribution is still abnormal in these embryos.

Complementation and Rescue Data
Rescued by
Partially rescued by
Comments

Pvrtub.PB completely rescues the macrophage aggregation and distribution phenotypes of stage 16 Pvr1 homozygous embryos.

Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL4srp.Hemo rescues the macrophage aggregation phenotype of stage 16 Pvr1 homozygous embryos. However, these embryos retain a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Isolated in an EMS screen using FRT40/FRT40 males, recovering single chromosomes and testing for lethality and sterility over Df(2L)TE29Aa-1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)