FlyBase Allele Report: Dmel\Pten[KK109278]

General Information

Symbol

Dmel\Pten^KK109278

Species

D. melanogaster

Name

FlyBase ID

FBal0236192

Feature type

allele

Associated gene

Dmel\Pten

Associated Insertion(s)

Carried in Construct

P{KK109278}

Also Known As

UAS-Pten-RNAi, PTEN^RNAi, PTEN-RNAi, PTEN RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK109278}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK109278

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Parniewska and Stocker, 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates chromosome 16p11.2 deletion syndrome, 593-kb

modeled by Pp4-19C^GD9561

(Iyer et al., 2018)

model of cancer

is exacerbated by B52^KK108253

(Parniewska and Stocker, 2020)

is ameliorated by Zrf1^KK102428

(Parniewska and Stocker, 2020)

is ameliorated by Girdin^KK101725

(Parniewska and Stocker, 2020)

is ameliorated by Mo25^GD11213

(Parniewska and Stocker, 2020)

is ameliorated by Osbp^KK107784

(Parniewska and Stocker, 2020)

is ameliorated by SF2^GD12097

(Parniewska and Stocker, 2020)

is ameliorated by SF2^HM05199

(Parniewska and Stocker, 2020)

is ameliorated by S6k^KK107986

(Parniewska and Stocker, 2020)

is ameliorated by SRPK^KK100641

(Parniewska and Stocker, 2020)

is ameliorated by Slik^GD11338

(Parniewska and Stocker, 2020)

is ameliorated by SF2^HMS00358

(Parniewska and Stocker, 2020)

is ameliorated by chico^KK107581

(Parniewska and Stocker, 2020)

is exacerbated by Srp54^GD10663

(Parniewska and Stocker, 2020)

is ameliorated by cic^KK112641

(Parniewska and Stocker, 2020)

is ameliorated by Vha100-1^KK105748

(Parniewska and Stocker, 2020)

is ameliorated by brp^KK108677

(Parniewska and Stocker, 2020)

is ameliorated by mask^KK100529

(Parniewska and Stocker, 2020)

is ameliorated by mib1^GD11786

(Parniewska and Stocker, 2020)

is ameliorated by ttv^GD1993

(Parniewska and Stocker, 2020)

is ameliorated by zfh1^KK109931

(Parniewska and Stocker, 2020)

is ameliorated by tra2^KK108747

(Parniewska and Stocker, 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Pten^KK109278 under the control of Scer\GAL4^en-e16E leads to increased cell death in third instar larval wing discs.

(Sun et al., 2020)

Expression of Pten^KK109278 under the control of Scer\GAL4^hh.PU results in increased size of the posterior compartment in in third instar larval wing discs.

(Romero-Pozuelo et al., 2017)

Expressing Pten^KK109278 under the control of Scer\GAL4^GMR.PU leads to a subtle rough eye phenotype.

(Iyer et al., 2016)

Expression of Pten^KK109278 in the eye imaginal disc, under the control of Scer\GAL4^GMR.PU results in a slight enlargement of the eye, compared to controls.

Knockdown of Pten in the dorsal compartment of the eye, through expression of Pten^KK109278 under the control of Scer\GAL4^mirr-DE results in an overall increase in eye size, as well as a reduction in the size of the ventral part, as compared to control eyes. This growth-reducing effect within the eye is visible under standard conditions but further enhanced on 10g/l yeast food.

RNAi-mediated knockdown of Pten does not result in enhanced tissue overgrowth under starvation conditions.

(Nowak et al., 2013)

Expression of Pten^KK109278 using Scer\GAL4^Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

(Pircs et al., 2012)

Expression of Pten^KK109278 in wild-type tissue results in slight tissue overgrowth.

(Willecke et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pten^KK109278, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of neoplasia phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689

(Willecke et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Pten^KK109278, Scer\GAL4^GMR.PU is a suppressor of eye | nutrition conditional phenotype of Scer\GAL4^GMR.PU, foxo^EP35-147

(Nowak et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Pten^KK109278 and pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.

(Gerlach et al., 2018)

The small eye phenotype found upon expression of foxo^EP35-147 is suppressed by knockdown of Pten through expression of Pten^KK109278 (with both constructs under the control of Scer\GAL4^GMR.PU).

(Nowak et al., 2013)

Depletion of Pten, through expression of Pten^KK109278, increases tumour size in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM clones.

(Willecke et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v101475

P{KK109278}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PTEN^dpten

(Iyer et al., 2018)

Pten^KK109278

(Singh et al., 2020)

Pten^Ri

(Gupta and Stocker, 2020)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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