FlyBase Allele Report: Dmel\mib1[GD11786]

General Information

Symbol

Dmel\mib1^GD11786

Species

D. melanogaster

Name

FlyBase ID

FBal0210070

Feature type

allele

Associated gene

Dmel\mib1

Associated Insertion(s)

Carried in Construct

P{GD11786}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11786}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11786

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Pten^KK109278

(Parniewska and Stocker, 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^Ser.PGF

(Hamel et al., 2010)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Ser.PGF

(Hamel et al., 2010)

Detailed Description

Statement

Reference

Expression of mib1^GD11786 under the control of Scer\GAL4^Ser.PGF post-embryonically (animals are transferred to 28[o]C after embryogenesis to inactivate Scer\GAL80^ts.αTub84B which is present in the stock) results in a mutant wing phenotype.

(Hamel et al., 2010)

Adults expressing mib1^GD11786 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 30-40% or 70-80% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11786

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, mib1^GD11786 has visible phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^Ser.PGF

(Hamel et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, mib1^GD11786 has wing phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^Ser.PGF

(Hamel et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of α4GT1^GS2078 results in strong suppression of the wing phenotype caused by expression of mib1^GD11786 under the control of Scer\GAL4^Ser.PGF post-embryonically (animals are transferred to 28[o]C after embryogenesis to inactivate Scer\GAL80^ts.αTub84B which is present in the stock).

(Hamel et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v27525