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General Information
Symbol
Dmel\ttvGD1993
Species
D. melanogaster
Name
FlyBase ID
FBal0210816
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ttv-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ttvGD1993 under the control of Scer\GAL4elav-C155 leads to a net increase in autophagy (assessed by proteolytic cleavage of mCherry-Atg8a) relative to controls.

Expression of ttvGD1993 in larval epidermal cells under the control of any following: Scer\GAL4A58, Scer\GAL4hh-Gal4, Scer\GAL4GMR10C12 or Scer\GAL4GMR16D01 results in severe reduction in terminal dendrite density as well as terminal dendrite number (with all except the Scer\GAL4A58 driver) within the intersegmental area in the adjacent class IV dendritic arborizing (da) neurons; the effect is apparent in first instar larvae already (at 24hr after egg laying, AEL) and becomes more pronounced as the larvae develop. Some differences in the dendrite length are observed also in the other three classes of da neurons but are much milder than in class IV neurons: The total dendrite length is slightly reduced in class I ddaD neurons but increased in class I ddaE neurons and in class II ddaB neurones (all with Scer\GAL4A58 driver), in class III ddaA neurons a slight decrease is observed in the dendritic tree length overall, as well as in total length and number of terminal dendrites, whereas no significant differences in any of these categories are found in ddaF neurons (with the Scer\GAL4hh-Gal4 driver) relative to control larvae. Long-term time-lapse imaging analysis of dendrite dynamics in larval class IV da neurons between 72 hr and 84 hr AEL in Scer\GAL4hh-Gal4>ttvGD1993 animals revealed that the high-order dendrites are highly dynamic at the border of the ttv knock-down zone - they can extend relatively far into this zone but are only transient and there is no obvious net increase in dendrite number or density over time. This is however not due to an intrinsic inability of da dendrites to permanently invade empty spaces at this stage.

The areas of dendritic arbor of class IV neurons directly adjacent to wild-type epidermal cells that are completely surrounded by flip-out clones of epidermal cells expressing ttvGD1993 under the control of Scer\GAL4Act5C.PI do not display any decrease in the density of terminal dendrites on the contrary, the density is significantly increased compared to controls with wild-type clones.

Adults expressing ttvGD1993 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 30-40% of the Scer\GAL4pnr-MD237 expression domain.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4pnr-MD237 expression domain.

Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 40-50% of the Scer\GAL4pnr-MD237-expressing area of the notum.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Suppressor of
Phenotype Manifest In
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of ttvGD1993 under the control of Scer\GAL4elav-C155 partially suppresses decreased eye size phenotype, while it fails to suppress disruption of eye patterning phenotype of expression of Psn541.UAS under the control of Scer\GAL4elav-C155.

Xenogenetic Interactions
Statement
Reference

Expression of ttvGD1993 enhances the eye phenotype seen when Hsap\INSC96Y.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
ttvGD1993
Name Synonyms
Secondary FlyBase IDs
    References (10)