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General Information
Symbol
Dmel\InRA1325D.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0156362
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-InRA1325D, UAS-InRCA, inRCA, InRCA, InRA1325D, UAS-InRact, UAS-InRAC
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a constitutively active form of InR (carries the amino acid replacement A1325D). The mutation mimics the human V938D protein variant of Longo et al., 1992 (PMID:1618747).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of InRA1325D.UAS under the control of Scer\GAL4Su(H).GBE (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to an increase in the cell size of enteroblasts and an increase in intestinal stem cell proliferation in adult guts when compared to controls.

Expression of InRA1325D.UAS under the control of Scer\GAL4GMR.PF leads to an increase in eye size when compared to controls.

Expression of InRA1325D.UAS under the control of Scer\GAL4Tub.PU in MARCM clones leads to a significant decrease in midgut cell number when compared to control adults.

Expression of InRA1325D.Scer\UAS under the control of Scer\GAL4esg-NP5130 combined with leads to a significant increase in the number of proliferating (EdU+) cells in the adult posterior midgut.

Clonal expression of InRA1325D.UAS under the control of Scer\GAL4Act5C.PP in the larval fat body induces larger nuclei, as compared to neighboring control cells.

Expression of InRA1325D.Scer\UAS under the control of Scer\GAL4insc-Mz1407 is capable of driving central brain neuroblast reactivation in the absence of dietary amino acids.

Expression of InRA1325D.Scer\UAS under the control of Scer\GAL4esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant increase in the number of mitotic cells in the midgut, as compared to controls.

Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent the normal cell cycle exit of pupal neuroblasts.

Expression of InRA1325D.Scer\UAS in the posterior wing compartment under the control of Scer\GAL4en-e16E does not affect wing vein formation.

Expression of InRA1325D.Scer\UAS under the control of Scer\GAL4GMR.PF does not produce R8 photoreceptor cell phenotypes.

Central nervous system neuroblasts of fed larvae expressing InRA1325D.Scer\UAS under the control of Scer\GAL4nab show an increase in EdU incorporation compared to controls.

Central nervous system neuroblasts of larvae expressing InRA1325D.Scer\UAS under the control of Scer\GAL4nab and raised on a diet lacking amino acids for 24 hours show an increase in EdU incorporation compared to controls.

Expression of InRA1325D.Scer\UAS driven by Scer\GAL4ap-md544 in dorsal cells of the wing causes an overgrowth phenotype in which the wing bends downward.

Overexpression of InRA1325D.Scer\UAS via Scer\GAL4GMR.PF causes a hyperplastic phenotype in the eye.

Third instar larvae expressing InRA1325D.Scer\UAS under the control of Scer\GAL4r4 have a significantly increased triglyceride/protein ratio compared to controls.

No scutellar bristle phenotypes are seen when InRA1325D.Scer\UAS is expressed under the control of Scer\GAL4sca-537.4. Expression in the wing under the control of Scer\GAL4en-e16E leads to over-growth of the posterior compartment.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The increase in the number of proliferating (EdU+) cells observed in the posterior midgut of adult flies upon expression of InRA1325D.Scer\UAS driven by Scer\GAL4esg-NP5130 (combined with Gal80[ts] for temporal control) is completely abolished by co-expression of Sam-SKK107709.

Larval fat body clones co-expressing Tl10b.UAS.cUa and InRA1325D.UAS under the control of Scer\GAL4Act5C.PP, however, do not show exhibit the nucleus sizes increases and decreases respectively observed upon single expressions of Akt1UAS.Tag:Myr(Unk) and Tl10b.UAS.cUa.

The ability of Scer\GAL4insc-Mz1407-driven expression of InRA1325D.Scer\UAS to induce central brain neuroblast reactivation in the absence of dietary amino acids is prevented by co-expression of ChroKK105370.

Co-expression of bunGD1392 suppresses the increased number of mitotic cells seen in the midguts of flies expressing InRA1325D.Scer\UAS under the control of Scer\GAL4esg-NP5130.

The increased cell-death phenotype of CskQ156Stop/CskQ156Stop somatic clones (induced specifically in the eye disc) expressing Ras85DV12.Scer\UAS under Scer\GAL4Act.PU is fully suppressed by co-expression of InRA1325D.Scer\UAS, resulting in tumor overgrowth of the eye disc in nearly all larvae. Majority of the larvae fail to pupariate, the ones that manage do so with a significant delay.

Expression of InRA1325D.Scer\UAS enhances the dendrite pruning defects seen in ddaC neurons expressing skpAGD9251 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

Expression of InRA1325D.Scer\UAS under the control of Scer\GAL4ppk.PG enhances the dendrite pruning defects seen in Mical15256 mutant ddaC neuron clones at 16 hours after puparium formation.

Expression of InRA1325D.Scer\UAS enhances the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF, resulting in an increase in the size of the blob-like structures seen in the medulla M1 layer. Many of the R8 axons terminate before reaching their final destination, the M3 layer. A number of axons also stop in the medulla M1 layer.

Co-expression of Sesnd04539 with InRA1325D.Scer\UAS via Scer\GAL4GMR.PF suppresses the InRA1325D.Scer\UAS hyperplastic eye phenotype.

Co-expression of Sesnd04539 with InRA1325D.Scer\UAS via Scer\GAL4ap-md544 suppresses the InRA1325D.Scer\UAS hyperplastic wing phenotype.

Xenogenetic Interactions
Statement
Reference

Expression of InRA1325D.Scer\UAS is unable to suppress the growth phenotypes seen in the wings and bristles of flies expressing Hsap\TGIF2LXScer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E or Scer\GAL4sca-537.4 respectively.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
InRA1325D.Scer\UAS
InRA1325D.UAS
Name Synonyms
Secondary FlyBase IDs
    References (23)