FlyBase Allele Report: Dmel\zfh2[GD4506]

General Information

Symbol

Dmel\zfh2^GD4506

Species

D. melanogaster

Name

FlyBase ID

FBal0210937

Feature type

allele

Associated gene

Dmel\zfh2

Associated Insertion(s)

Carried in Construct

P{GD4506}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4506}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4506

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult gut, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

antenna, with Scer\GAL4^en.PU

(Guntur et al., 2021)

arista, with Scer\GAL4^en.PU

(Guntur et al., 2021)

enteroblast | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

haltere, with Scer\GAL4^en.PU

(Guntur et al., 2021)

intestinal stem cell | adult stage | chemical conditional, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

leg, with Scer\GAL4^en.PU

(Guntur et al., 2021)

leg joint, with Scer\GAL4^en.PU

(Guntur et al., 2021)

nucleus | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

plasma membrane | adult stage | chemical conditional, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

segment of leg, with Scer\GAL4^en.PU

(Guntur et al., 2021)

tibia-tarsus joint, with Scer\GAL4^en.PU

(Guntur et al., 2021)

Detailed Description

Statement

Reference

Expression of zfh2^GD4506 under the control of Scer\GAL4^Su(H).GBE (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to a decrease in the cell size and nuclear size of enteroblasts in adult guts when compared to controls. It prevents Dextran Sulfate Sodium (DSS)-induced enteroblast activation (decreased cell circulatory and increased number of cells with membrane protrusions) and also impairs DSS-induced intestinal stem cell proliferation compared to control adults.

(Rojas Villa et al., 2019)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development