Open Close
General Information
Symbol
Dmel\gogoScer\UAS.T1
Species
D. melanogaster
Name
FlyBase ID
FBal0297301
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-gogoT1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Scer\UAS drives expression of untagged, full-length gogo.

Scer\UAS regulatory sequences drive expression of full length gogo, which is tagged with four copies of T:Hsap\MYC.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When rhabdomere R8 terminals are prevented from reaching medulla layer M3 through expression of ActβGD3157 in the eye under the control of Scer\GAL4GMR.long, Tm20 neurons exhibit dendritic patterning defects.Dendrite terminating frequency is reduced as compared with wild type.

Expression of gogoScer\UAS.T1 under the control of Scer\GAL4GMR.PF results in R8 photoreceptors that exhibit blob-like structures at the medulla M1 layer. No mistargeting of R7 photoreceptors is seen.

When gogoScer\UAS.T1 is expressed using Scer\GAL4GMR.PU, the medulla looks normal overall. However, R8 axons show abnormally thick swellings at the M1 layer and at their termini in the M3 layer.

Expression of Scer\GAL4GMR.PU under the control of gogoFL.Scer\UAS.T:Hsap\MYC results in photoreceptor cell R8 axons forming large bulb-like structures at the M1 layer, where they terminate.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Ablation of rhabdomere R7 neurons with sev4 does not modify the Tm20 dendritic patterning defects seen when R8 terminals are stalled in medulla layer M1 through expression of gogoFL.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.long.

Expression of ActβGD3157 does not significantly enhance the Tm20 dendritic patterning defects seen when rhabdomere R8 terminals are stalled in medulla layer M1 through expression of gogoFL.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.long.

R7 photoreceptor axons that over-express gogoScer\UAS.T1 and stanScer\UAS.cUa (under the control of Scer\GAL4GMR.PF) are redirected to the M3 layer.

Expression of InRScer\UAS.cHa strongly enhances the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF, resulting in an increase in the size of the blob-like structures seen in the medulla M1 layer. Premature axon stopping is also visible.

Expression of InRK1409A.Scer\UAS completely suppresses the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF. No blob-like structures are seen in the medulla M1 layer.

Expression of InRA1325D.Scer\UAS enhances the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF, resulting in an increase in the size of the blob-like structures seen in the medulla M1 layer. Many of the R8 axons terminate before reaching their final destination, the M3 layer. A number of axons also stop in the medulla M1 layer.

One copy of InRex15 suppresses the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF. 12% of R8 axons form blob-like structures at the M1 layer.

One copy of each of Ilp54 and Df(3L)Ilp1-4 suppresses the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF. 6% of R8 axons form blob-like structures at the M1 layer.

Co-expression of gogoFL.Scer\UAS.T:Hsap\MYC and stanScer\UAS.cUa under the control of Scer\GAL4GMR.PU results in R7 photoreceptor cell axons mistargeting to the M3 layer of the medulla.

Co-expression of gogoFL.Scer\UAS.T:Hsap\MYC and stanΔIntra.Scer\UAS under the control of Scer\GAL4GMR.PU results in a strong premature axon stopping phenotype in the R7 photoreceptor cells (41.0%).

R8 axon swelling seen in Scer\GAL4GMR.PU, gogoScer\UAS.T1 animals is suppressed when htsScer\UAS.Add1.T:Hsap\MYC is co-expressed. Likewise, the R8 axon swelling seen in Scer\GAL4GMR.PU, htsScer\UAS.Add1.T:Hsap\MYC animals is suppressed when gogoScer\UAS.T1 is co-expressed.

R8 axon swelling seen in Scer\GAL4GMR.PU, gogoScer\UAS.T1 animals is suppressed when htsGMR.Add1 is also present. Likewise, the R8 axon swelling seen in htsGMR.Add1 animals is suppressed when Scer\GAL4GMR.PU, gogoScer\UAS.T1 is present.

The number and size of R8 axon swellings seen in Scer\GAL4GMR.PU, gogoScer\UAS.T1 animals is increased in a htsnull/+ background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The photoreceptor axon projection defects seen in animals in which the retina is homozygous for gogoD869 (induced using the eyFLP method) are not rescued by expression of gogoFL.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4sev.PM181.

The abnormal bundling behaviour of mutant axons in small homozygous gogoD869 clones of R7/R8 photoreceptor cells is rescued by expression of gogoFL.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PU.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
gogoFL.Scer\UAS.T:Hsap\MYC
gogoScer\UAS.T1
Name Synonyms
Secondary FlyBase IDs
  • FBal0242633
  • FBal0248994
References (9)