FlyBase Allele Report: Dmel\gogo[Scer\UAS.T1]

General Information

Symbol

Dmel\gogo^Scer\UAS.T1

Species

D. melanogaster

Name

FlyBase ID

FBal0297301

Feature type

allele

Associated gene

Dmel\gogo

Associated Insertion(s)

Carried in Construct

P{UAS-gogo.T1}

Also Known As

UAS-gogoT1

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Ohler et al., 2011, Tomasi et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-gogo.T1}

Cytology

Description

Scer\UAS drives expression of untagged, full-length gogo.

(Ohler et al., 2011)

Scer\UAS regulatory sequences drive expression of full length gogo, which is tagged with four copies of T:Hsap\MYC.

(Tomasi et al., 2008)

Allele components

Component

Use(s)

Encoded product / tool

gogo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GMR.long

(Ting et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^GMR.PF

(Mann et al., 2012)

Phenotype Manifest In

medulla layer M1, with Scer\GAL4^GMR.PF

(Mann et al., 2012)

photoreceptor cell R8, with Scer\GAL4^GMR.PF

(Mann et al., 2012)

photoreceptor cell R8, with Scer\GAL4^GMR.PU

(Ohler et al., 2011, Tomasi et al., 2008)

transmedullary neuron Tm20, with Scer\GAL4^GMR.long

(Ting et al., 2014)

Detailed Description

Statement

Reference

When rhabdomere R8 terminals are prevented from reaching medulla layer M3 through expression of Actβ^GD3157 in the eye under the control of Scer\GAL4^GMR.long, Tm20 neurons exhibit dendritic patterning defects.Dendrite terminating frequency is reduced as compared with wild type.

(Ting et al., 2014)

Expression of gogo^Scer\UAS.T1 under the control of Scer\GAL4^GMR.PF results in R8 photoreceptors that exhibit blob-like structures at the medulla M1 layer. No mistargeting of R7 photoreceptors is seen.

(Mann et al., 2012)

When gogo^Scer\UAS.T1 is expressed using Scer\GAL4^GMR.PU, the medulla looks normal overall. However, R8 axons show abnormally thick swellings at the M1 layer and at their termini in the M3 layer.

(Ohler et al., 2011)

Expression of Scer\GAL4^GMR.PU under the control of gogo^{FL.Scer\UAS.T:Hsap\MYC} results in photoreceptor cell R8 axons forming large bulb-like structures at the M1 layer, where they terminate.

(Tomasi et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, enhanceable by InR^A1325D.UAS, Scer\GAL4^GMR.PF

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, enhanceable by InR^UAS.cHa, Scer\GAL4^GMR.PF

(Mann et al., 2012)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.long, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, non-enhanceable by sev⁴

(Ting et al., 2014)

Scer\GAL4^GMR.long, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, non-enhanceable by Actβ^GD3157, Scer\GAL4^GMR.long

(Ting et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, suppressible by InR[+]/InR^ex15

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, suppressible by Ilp5⁴/Ilp5[+]/Df(3L)Ilp1-4/+

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, suppressible by InR^K1409A.UAS, Scer\GAL4^GMR.PF

(Mann et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.long, gogo^Scer\UAS.T1 has abnormal neuroanatomy phenotype, non-suppressible by sev⁴

(Ting et al., 2014)

Other

Statement

Reference

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1, stan^UAS.cUa has abnormal neuroanatomy phenotype

(Berger-Müller et al., 2013)

Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1, stan^UAS.cUa has abnormal neuroanatomy phenotype

(Hakeda-Suzuki et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, enhanceable by InR^UAS.cHa, Scer\GAL4^GMR.PF

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has medulla layer M1 phenotype, enhanceable by InR^UAS.cHa, Scer\GAL4^GMR.PF

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, enhanceable by InR^A1325D.UAS, Scer\GAL4^GMR.PF

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has medulla layer M1 phenotype, enhanceable by InR^A1325D.UAS, Scer\GAL4^GMR.PF

(Mann et al., 2012)

Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, enhanceable by hts[+]/hts^null

(Ohler et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.long, gogo^Scer\UAS.T1 has transmedullary neuron Tm20 phenotype, non-enhanceable by sev⁴

(Ting et al., 2014)

Scer\GAL4^GMR.long, gogo^Scer\UAS.T1 has transmedullary neuron Tm20 phenotype, non-enhanceable by Actβ^GD3157, Scer\GAL4^GMR.long

(Ting et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, suppressible by InR^K1409A.UAS, Scer\GAL4^GMR.PF

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, suppressible by InR[+]/InR^ex15

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, suppressible by Ilp5⁴/Ilp5[+]/Df(3L)Ilp1-4/+

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has medulla layer M1 phenotype, suppressible by InR[+]/InR^ex15

(Mann et al., 2012)

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1 has medulla layer M1 phenotype, suppressible by Ilp5⁴/Ilp5[+]/Df(3L)Ilp1-4/+

(Mann et al., 2012)

Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, suppressible by hts^{UAS.Add1.Tag:MYC}/Scer\GAL4^GMR.PU

(Ohler et al., 2011)

Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1 has photoreceptor cell R8 phenotype, suppressible by hts^GMR.Add1

(Ohler et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.long, gogo^Scer\UAS.T1 has transmedullary neuron Tm20 phenotype, non-suppressible by sev⁴

(Ting et al., 2014)

Suppressor of

Statement

Reference

gogo^Scer\UAS.T1/Scer\GAL4^GMR.PU is a suppressor of photoreceptor cell R8 phenotype of hts^GMR.Add1

(Ohler et al., 2011)

gogo^Scer\UAS.T1/Scer\GAL4^GMR.PU is a suppressor of photoreceptor cell R8 phenotype of Scer\GAL4^GMR.PU, hts^{UAS.Add1.Tag:MYC}

(Ohler et al., 2011)

Other

Statement

Reference

Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1, stan^UAS.cUa has photoreceptor cell R7 phenotype

(Berger-Müller et al., 2013)

Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1, stan^UAS.cUa has photoreceptor cell R7 phenotype

(Hakeda-Suzuki et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Ablation of rhabdomere R7 neurons with sev⁴ does not modify the Tm20 dendritic patterning defects seen when R8 terminals are stalled in medulla layer M1 through expression of gogo^{FL.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.long.

Expression of Actβ^GD3157 does not significantly enhance the Tm20 dendritic patterning defects seen when rhabdomere R8 terminals are stalled in medulla layer M1 through expression of gogo^{FL.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.long.

(Ting et al., 2014)

R7 photoreceptor axons that over-express gogo^Scer\UAS.T1 and stan^Scer\UAS.cUa (under the control of Scer\GAL4^GMR.PF) are redirected to the M3 layer.

(Berger-Müller et al., 2013)

Expression of InR^Scer\UAS.cHa strongly enhances the R8 photoreceptor phenotype seen when gogo^Scer\UAS.T1 is expressed under the control of Scer\GAL4^GMR.PF, resulting in an increase in the size of the blob-like structures seen in the medulla M1 layer. Premature axon stopping is also visible.

Expression of InR^{K1409A.Scer\UAS} completely suppresses the R8 photoreceptor phenotype seen when gogo^Scer\UAS.T1 is expressed under the control of Scer\GAL4^GMR.PF. No blob-like structures are seen in the medulla M1 layer.

Expression of InR^{A1325D.Scer\UAS} enhances the R8 photoreceptor phenotype seen when gogo^Scer\UAS.T1 is expressed under the control of Scer\GAL4^GMR.PF, resulting in an increase in the size of the blob-like structures seen in the medulla M1 layer. Many of the R8 axons terminate before reaching their final destination, the M3 layer. A number of axons also stop in the medulla M1 layer.

One copy of InR^ex15 suppresses the R8 photoreceptor phenotype seen when gogo^Scer\UAS.T1 is expressed under the control of Scer\GAL4^GMR.PF. 12% of R8 axons form blob-like structures at the M1 layer.

One copy of each of Ilp5⁴ and Df(3L)Ilp1-4 suppresses the R8 photoreceptor phenotype seen when gogo^Scer\UAS.T1 is expressed under the control of Scer\GAL4^GMR.PF. 6% of R8 axons form blob-like structures at the M1 layer.

(Mann et al., 2012)

Co-expression of gogo^{FL.Scer\UAS.T:Hsap\MYC} and stan^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PU results in R7 photoreceptor cell axons mistargeting to the M3 layer of the medulla.

Co-expression of gogo^{FL.Scer\UAS.T:Hsap\MYC} and stan^{ΔIntra.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in a strong premature axon stopping phenotype in the R7 photoreceptor cells (41.0%).

(Hakeda-Suzuki et al., 2011)

R8 axon swelling seen in Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1 animals is suppressed when hts^{Scer\UAS.Add1.T:Hsap\MYC} is co-expressed. Likewise, the R8 axon swelling seen in Scer\GAL4^GMR.PU, hts^{Scer\UAS.Add1.T:Hsap\MYC} animals is suppressed when gogo^Scer\UAS.T1 is co-expressed.

R8 axon swelling seen in Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1 animals is suppressed when hts^GMR.Add1 is also present. Likewise, the R8 axon swelling seen in hts^GMR.Add1 animals is suppressed when Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1 is present.

The number and size of R8 axon swellings seen in Scer\GAL4^GMR.PU, gogo^Scer\UAS.T1 animals is increased in a hts^null/+ background.

(Ohler et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/gogo^Scer\UAS.T1 rescues gogo^D869

(Tomasi et al., 2008)

Fails to rescue

gogo^Scer\UAS.T1/Scer\GAL4^sev.PM181 fails to rescue gogo^D869

(Tomasi et al., 2008)

Comments

The photoreceptor axon projection defects seen in animals in which the retina is homozygous for gogo^D869 (induced using the eyFLP method) are not rescued by expression of gogo^{FL.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sev.PM181.

The abnormal bundling behaviour of mutant axons in small homozygous gogo^D869 clones of R7/R8 photoreceptor cells is rescued by expression of gogo^{FL.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU.

(Tomasi et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

gogo^{FL.Scer\UAS.T:Hsap\MYC}

gogo^Scer\UAS.T1

Name Synonyms

Secondary FlyBase IDs

FBal0242633
FBal0248994

References (9)

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