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General Information
Symbol
Dmel\InRUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Huang
FlyBase ID
FBal0102968
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-InR, UAS-dInR, UAS-dinrWT, UAS-Inrwt
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of InR is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Under control nutrition conditions, the adulthood-only expression of InRUAS.cHa under the combined control of Scer\GAL4C587 and Gal80[ts] does not lead to any significant changes in the number of escort cells in the germarium, as compared to controls.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4phm.PO does not significantly change the number of lipid droplets in the prothoracic gland cells of third instar larvae compared to controls.

Wild type males spend significantly more courtship time with females expressing InRScer\UAS.cHa under the control of Scer\GAL4αTub84B.Switch.PK (upon exposure to RU486), as compared to control females. The same effect on female attractiveness is seen when cuticular hydrocarbons from the aforementioned females are used to "perfume" same-age oenocyte-less flies.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4GMR.PF does not produce R8 photoreceptor cell phenotypes.

Overexpression of InRScer\UAS.cHa in fat body cell clones under the control of Scer\GAL4αTub84B.PP leads to an enormous fat body cell overgrowth phenotype. This is accompanied by a significant reduction in lipid droplet size compared with wild-type.

Fat body clones expressing InRScer\UAS.cHa under the control of Scer\GAL4αTub84B.PP show an increase in cell size.

Overexpression of InRScer\UAS.cHa under the control of Scer\GAL4GMR.PF results in eye hyperplasia.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4GMR.PF leads to an increase in ommatidia size.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4ptc-559.1 results in a weak but consistent expansion of the distance between wing veins L3 and L4.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4tim.Scer\UAS, dramatically increases sensitivity to oxidative stress. These flies die within 2 days of being challenged with 1mM paraquat. When treated with 0.05-0.1mM paraquat they survive up to 6 days, with the majority showing arrhythmic locomotor behavior.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4Switch1.106, increases sensitivity of behavioral rhythms to paraquat.

Expression of InRScer\UAS.cHa in border cells, driven by Scer\GAL4slbo.2.6 does not affect the formation of the egg chamber apical cap or the migration of the border cell cluster to the oocyte.

Pharate adults expressing InRScer\UAS.cHa under the control of Scer\GAL4phm.PO are 42% of the weight of controls when larvae have been raised in constant light.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 results in ectopic bristles and an excess of sensilla trichodea in the most proximal part of the coxa, ectopic bristles in the arista and third antennal segment, ectopic bristles in the capitellum and occasionally in the pedicellum of the haltere and ectopic thorn bristles in the female genitalia.

Cells in InRScer\UAS.cHa; Scer\GAL4Act5C.PP somatic clonesi n the larval fat body overgrow dramatically.

The eyes of InRScer\UAS.cHa; Scer\GAL4GMR.PF flies have massive overgrowth.

Flies that express InRScer\UAS.cHa under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa show a consistently low heart rate across all ages compared to wild-type flies. These flies show an increased rate of stress-induced heart failure at the age of one week compared to in wild type. The rate of this heart failure does increase with age, but at a markedly reduced magnitude compared to wild type. These effects are true for both male and female.

Almost all endogenous caspase activation is blocked in InRScer\UAS.cHa; Scer\GAL4GMR.PF eye discs of third instar larvae and 30 hours APF pupae. This is accompanied by an increase in photoreceptor differentiation at both of these stages.

Clones of cells expressing InRScer\UAS.cHa under the control of Scer\GAL4Act5C.PP in the gut, fat body, Malpighian tubules and epidermis are only slightly larger than control cells at the time of larval hatching. After several days of starvation on 20% sucrose, these cells are much larger than adjacent control cells and have a visibly increased DNA content. Gut and fat body cells expressing InRScer\UAS.cHa under the control of Scer\GAL4Act5C.PP continue to replicate their DNA for at least 2-3 days under starvation conditions. Expression of InRScer\UAS.cHa in third larval instar fat body cells under the control of Scer\GAL4Act5C.PP increases the opacity of the cytoplasm, thus promoting nutrient storage. The cytoplasm of the cells contains many more, but much smaller, lipid droplets than neighbouring control cells. Some fed animals expressing InRScer\UAS.cHa under the control of Scer\GAL4Adh.PF survive to the third larval instar and a few viable adults eclose. In contrast, expression of InRScer\UAS.cHa under the control of Scer\GAL4Adh.PF in starved animals results in first instar larvae dying within 3-4 days of hatching (control animals survive 8-9 days). Animals expressing InRScer\UAS.cHa under the control of Scer\GAL4Adh.PF feed poorly, often having no food in their gut, and frequently wander away from their food.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4Act5C.PP in clones results in an increase in cell size and an increase in clone area compared to controls.

InRScer\UAS.cHa when driven by Scer\GAL4ey.PH causes hyperproliferative outgrowth and an increase in cell size in the eye. A relatively normal ommatidial arrangement and architecture are retained.

When InRScer\UAS.cHa is driven by Scer\GAL4GMR.PF the flies have enlarged eyes due to an increase in cell number and cell size.

Expression of InRScer\UAS.cHa under the control of Scer\GAL4ey.PH results in lethality at 25oC and a dramatic increase in ommatidia number in escapers at room temperature.

When expression is driven by Scer\GAL4ey.PH at 25oC lethality results. At room temperature, a few flies survive with over proliferated eyes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of InRScer\UAS.cHa strongly enhances the R8 photoreceptor phenotype seen when gogoScer\UAS.T1 is expressed under the control of Scer\GAL4GMR.PF, resulting in an increase in the size of the blob-like structures seen in the medulla M1 layer. Premature axon stopping is also visible.

No R8 photoreceptor phenotypes are seen when gogoFFD.Scer\UAS and InRScer\UAS.cHa are co-expressed under the control of Scer\GAL4GMR.PF.

Expression of InRScer\UAS.cHa slightly enhances the R8 axon stopping phenotype seen when gogoDDD.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

The effects of Scer\GAL4αTub84B.PP>InRScer\UAS.cHa overexpression on cell growth and lipid droplet size in fat body cell clones are completely abrogated in a srlKG08646 mutant genetic background.

Ets97D613/Df(3R)ro80b does not significantly affect the increase in cell size seen in fat body clones expressing InRScer\UAS.cHa under the control of Scer\GAL4αTub84B.PP.

ImpL2EP5.66 suppresses the big eye phenotype resulting from the overexpression of InRScer\UAS.cHa driven by Scer\GAL4GMR.PF.

ImpL2MG2 fails to suppresses the big eye phenotype resulting from the overexpression of InRScer\UAS.cHa driven by Scer\GAL4GMR.PF.

ImpL2Scer\UAS.cHa partially suppresses the eye overgrowth resulting from the overexpression of InRScer\UAS.cHa driven by Scer\GAL4GMR.PF.

ImpL2s.Scer\UAS completely reverses the eye overgrowth resulting from the overexpression of InRScer\UAS.cHa driven by Scer\GAL4GMR.PF.

Expression of RpL8dsRNA.Scer\UAS partially suppresses the increase in ommatidia size seen when InRScer\UAS.cHa is expressed under the control of Scer\GAL4GMR.PF.

The distance between wing veins L3 and L4 is smaller than normal in flies co-expressing InRScer\UAS.cHa and TctpdsRNA.Scer\UAS under the control of Scer\GAL4ptc-559.1, resembling the phenotype seen in flies expressing TctpdsRNA.Scer\UAS alone under the control of Scer\GAL4ptc-559.1.

Overgrowth of cells in InRScer\UAS.cHa; Scer\GAL4Act5C.PP clones in the larval fat body is largely suppressed by hepAct.Scer\UAS.

The massive overgrowth of eyes seen in InRScer\UAS.cHa; Scer\GAL4GMR.PF flies is suppressed by B4EP7-66 or B4Scer\UAS.cSa. Embryonic lethality due to InRScer\UAS.cHa; Scer\GAL4en-e16E is also suppressed by B4Scer\UAS.cSa.

The addition of Df(3L)AC1 to InRScer\UAS.cHa, Scer\GAL4GMR.PB flies, suppresses the eye phenotype seen in these flies. The subsequent addition of Ilp2Scer\UAS.cBa reverts the suppression.

Coexpression of both gigScer\UAS.cTa and Tsc1Scer\UAS.cPa (but not either gigScer\UAS.cTa or Tsc1Scer\UAS.cPa alone) rescues the lethality and extra ommatidia phenotype of flies expressing InRScer\UAS.cHa under the control of Scer\GAL4ey.PH.

Eye size in animals expressing InRScer\UAS.cHa under the control of Scer\GAL4GMR.PF is restored almost to wild type by Pk61CEP3091/Pk61C4ΔEP, although eye roughness is increased.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\RPL8DN.Scer\UAS partially suppresses the increase in ommatidia size seen when InRScer\UAS.cHa is expressed under the control of Scer\GAL4GMR.PF.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
InRScer\UAS.cHa
InRUAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Huang
Secondary FlyBase IDs
    References (31)