FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\RPL8DN.UAS
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General Information
Symbol
Hsap\RPL8DN.UAS
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Xu
FlyBase ID
FBal0280381
Feature type
allele
Associated gene
Hsap\RPL8
Associated Insertion(s)
Carried in Construct
P{UAS-hRPL8.DN}
Key Links
Transgenic product class
frameshift_variant
(Xu et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
frameshift_variant
(Xu et al., 2008)
Mutagen
in vitro construct
(Xu et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-hRPL8.DN}
Cytology
Description

UAS regulatory sequences drive expression of a mutant form of Hsap\RPL8 containing a frameshift at G710, resulting in a truncation of the 257 amino acid full-length protein at glycine 236 and a fusion of an additional 52 amino acid residues to the C-terminus.

(Xu et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\RPL8
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When Hsap\RPL8DN.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF the eyes are small compared to wild type and appear flattened. This phenotype is not due to a reduction in the number of ommatidia, but due to a reduction in their size. The photoreceptor clusters are also smaller than those seen in controls but undergo normal neuronal differentiation in the posterior of the eye disc.

      Expression of Hsap\RPL8DN.Scer\UAS under the control of Scer\GAL4en-e16E causes a reduction in wing cell size, wing cell number and consequently wing size.

      The wing margin bristle cells in clones expressing Hsap\RPL8DN.Scer\UAS under the control of Scer\GAL4Scer\FRT.Act5C are reduced in size by 20% compared to controls.

      (Xu et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of RpL8Scer\UAS.cXa fully suppresses the small eye phenotype seen when Hsap\RPL8DN.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

      Expression of Hsap\RPL8DN.Scer\UAS partially suppresses the increase in ommatidia size seen when InRScer\UAS.cHa is expressed under the control of Scer\GAL4GMR.PF.

      Expression of Hsap\RPL8DN.Scer\UAS partially suppresses the increase in ommatidia size seen when Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PF.

      Expression of Hsap\RPL8DN.Scer\UAS under the control of Scer\GAL4GMR.PF partially suppresses the increase in eye and head capsule size seen in Tsc1Q600X mutant clones.

      Expression of Hsap\RPL8DN.Scer\UAS partially suppresses the increase in eye size seen when S6kScer\UAS.cWa is expressed under the control of Scer\GAL4GMR.PF.

      Expression of Hsap\RPL8DN.Scer\UAS under the control of Scer\GAL4GMR.PF enhances the reduction in eye size seen in S6kl-1 mutant clones.

      Expression of Hsap\RPL8DN.Scer\UAS partially suppresses the increase in eye size seen when dmScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF.

      (Xu et al., 2008)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\RPL8DN.Scer\UAS
      Hsap\RPL8DN.UAS
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Xu
      Secondary FlyBase IDs
        References (1)