FlyBase Allele Report: Dmel\Ets97D[613]

General Information

Symbol

Dmel\Ets97D⁶¹³

Species

D. melanogaster

Name

FlyBase ID

FBal0065250

Feature type

allele

Associated gene

Dmel\Ets97D

Associated Insertion(s)

Carried in Construct

Also Known As

delg⁶¹³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: Q193term.

(Schulz et al., 1993)

Amino acid replacement: I289T.

(Schulz et al., 1993)

Nucleotide substitution: C?T.

(Schulz et al., 1993)

Nucleotide substitution: T?C.

(Schulz et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:26,911,991..26,911,991 [-]

Nucleotide change:

C26911991T

Reported nucleotide change:

C?T

Amino acid change:

Q193term | Ets97D-PA; Q193term | Ets97D-PB

Reported amino acid change:

Q193term

Comment:

An additional T to C base change occurs in in Ets97D⁶¹³ at codon 289, which is after the nonsense mutation.

(Schulz et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Tiefenböck et al., 2010)

lethal | recessive

(Schulz et al., 1993)

lethal - all die before end of pupal stage (with Df(3R)ro80b)

(Baltzer et al., 2009)

Phenotype Manifest In

egg

(Schulz et al., 1993)

egg chorion

(Schulz et al., 1993)

fat body

(Tiefenböck et al., 2010)

fat body | third instar larval stage (with Df(3R)ro80b)

(Baltzer et al., 2009)

follicle cell

(Schulz et al., 1993)

mitochondrial chromosome

(Tiefenböck et al., 2010)

mitochondrion

(Tiefenböck et al., 2010)

mitochondrion | third instar larval stage (with Df(3R)ro80b)

(Baltzer et al., 2009)

nurse cell & nuclear chromosome

(Schulz et al., 1993)

Detailed Description

Statement

Reference

Compared with wild-type, the larval fat body of homozygous Ets97D⁶¹³ mutants show reduced mitochondrial abundance. The morphology of mitochondria appear normal in Ets97D⁶¹³ mutants.

Mitochondrial DNA content (normalised to nuclear DNA) in Ets97D⁶¹³ mutants is increased compared to that of controls.

Ets97D⁶¹³ mutants pupate with a one- or two-day delay compared with wild-type.

(Tiefenböck et al., 2010)

Ets97D⁶¹³/Df(3R)ro80b mutant fat body cells from third instar larvae contain a similar number of mitochondria to controls, but these mitochondria are strongly reduced in size. Despite the small size the morphology is unaffected. Levels of oxygen consumption, cytochrome c oxidase activity and cytochrome c protein levels are unaffected and the mitochondria have an increased mitochondrial membrane potential. The drop in oxygen consumption seen in controls in response to low-yeast food is still observed in Ets97D⁶¹³/Df(3R)ro80b mutants. In contrast to wild type, in which the mitochondrial area drops in response to low yeast food, no difference is seen between normal-fed Ets97D⁶¹³/Df(3R)ro80b mutants larvae and those on low-yeast food. No changes in mitochondrial abundance or morphology are seen in the gut, salivary gland, trachea or imaginal discs.

(Baltzer et al., 2009)

Ets97D⁶¹³/Df(3R)ro80b and Ets97D⁶¹³/Ets97D⁹⁰² flies die as pharate adults. Ets97D^tne-1 is viable but female sterile in transheterozygous combination with Ets97D⁶¹³. Ets97D^tne-1/Ets97D⁶¹³ females show abnormalities in oogenesis, including problems with nurse cell chromosome decondensation, follicle cell migration and chorion formation. Eggs obtained from these females show no obvious development.

(Schulz et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Ets97D⁶¹³ is an enhancer of abnormal developmental rate phenotype of srl^KG08646

(Tiefenböck et al., 2010)

NOT Enhancer of

Statement

Reference

Ets97D⁶¹³/Df(3R)ro80b is a non-enhancer of increased cell size | somatic clone phenotype of InR^UAS.cHa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

Suppressor of

Statement

Reference

Ets97D⁶¹³/Df(3R)ro80b is a suppressor | partially of increased cell size | somatic clone phenotype of Myc^UAS.cSAa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

Ets97D⁶¹³/Df(3R)ro80b is a suppressor of increased cell size | somatic clone phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

NOT Suppressor of

Statement

Reference

Ets97D⁶¹³/Df(3R)ro80b is a non-suppressor of increased cell size | somatic clone phenotype of InR^UAS.cHa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ets97D⁶¹³ has fat body phenotype, enhanceable by srl^KG08646

(Tiefenböck et al., 2010)

Ets97D⁶¹³ has mitochondrion phenotype, enhanceable by srl^KG08646

(Tiefenböck et al., 2010)

Ets97D⁶¹³ has mitochondrial chromosome phenotype, enhanceable by srl^KG08646

(Tiefenböck et al., 2010)

NOT Enhanced by

Statement

Reference

Ets97D⁶¹³/Df(3R)ro80b has mitochondrion | third instar larval stage phenotype, non-enhanceable by Cdk4³

(Baltzer et al., 2009)

Ets97D⁶¹³/Df(3R)ro80b has fat body | third instar larval stage phenotype, non-enhanceable by Cdk4³

(Baltzer et al., 2009)

NOT Enhancer of

Statement

Reference

Ets97D⁶¹³/Df(3R)ro80b is a non-enhancer of mitochondrion | third instar larval stage phenotype of Cdk4³

(Baltzer et al., 2009)

Ets97D⁶¹³/Df(3R)ro80b is a non-enhancer of fat body | third instar larval stage phenotype of Cdk4³

(Baltzer et al., 2009)

Ets97D⁶¹³/Df(3R)ro80b is a non-enhancer of fat body | somatic clone phenotype of InR^UAS.cHa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

Suppressor of

Statement

Reference

Ets97D⁶¹³/Df(3R)ro80b is a suppressor | partially of fat body | somatic clone phenotype of Myc^UAS.cSAa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

Ets97D⁶¹³/Df(3R)ro80b is a suppressor of fat body | somatic clone phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

NOT Suppressor of

Statement

Reference

Ets97D⁶¹³/Df(3R)ro80b is a non-suppressor of fat body | somatic clone phenotype of InR^UAS.cHa, Scer\GAL4^αTub84B.PP

(Baltzer et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

srl^KG08646, Ets97D⁶¹³ double mutants show a more severe mitochondrial phenotype in the fat body. The abundance of mitochondria in the fat body is further reduced compared with Ets97D⁶¹³ single mutants. In contrast to the phenotype in the single mutants, the mitochondria in double mutants are rounded in shape and the inner-mitochondrial cristae are either missing or strongly reduced in size.

Mitochondrial DNA content (normalised to nuclear DNA) in srl^KG08646, Ets97D⁶¹³ double mutants is further increased compared to that of Ets97D⁶¹³ single mutants.

The delay in pupation is increased to four days in srl^KG08646, Ets97D⁶¹³ double mutants.

(Tiefenböck et al., 2010)

Ets97D⁶¹³/Df(3R)ro80b does not significantly affect the increase in cell size seen in fat body clones expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^αTub84B.PP.

Ets97D⁶¹³/Df(3R)ro80b partially suppresses increase in cell size seen in fat body clones expressing Myc^{Scer\UAS.cSAa} under the control of Scer\GAL4^αTub84B.PP.

Ets97D⁶¹³/Df(3R)ro80b suppresses the increase in cell size seen in fat body clones co-expressing CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PP.

The reduced mitochondrial abundance seen in Cdk4³ or Ets97D⁶¹³/Df(3R)ro80b mutants alone is not enhanced in the double mutants.

(Baltzer et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ets97D⁶¹³/Df(3R)ro80b is rescued by Scer\GAL4^hs.PU/Ets97D^UAS.Tag:HA

(Baltzer et al., 2009)

Ets97D⁶¹³/Df(3R)ro80b is rescued by Scer\GAL4^ppl.PP/Ets97D^UAS.Tag:HA

(Baltzer et al., 2009)

Ets97D⁶¹³ is rescued by Ets97D^+t5.4

(Schulz et al., 1993)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ets97D⁶¹³

delg⁶¹³

(Tiefenböck et al., 2010, Baltzer et al., 2009)

l(3)613

(Schulz et al., 1993)

Name Synonyms

Secondary FlyBase IDs

References (3)

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