FlyBase Allele Report: Dmel\Cdk4[3]

General Information

Symbol

Dmel\Cdk4³

Species

D. melanogaster

Name

FlyBase ID

FBal0119691

Feature type

allele

Associated gene

Dmel\Cdk4

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Meyer et al., 2000)

Progenitor genotype

Cdk4^s4639

(Meyer et al., 2000)

Cytology

Description

Construct: Contains a 1.8kb deletion that removes much of the second intron in the coding region and at least two coding exons.

(Meyer et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

exacerbates tuberous sclerosis

modeled by Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Heterozygous Cdk4³ ameliorates the neoplasia seen in the btl::Egfr^{Scer\UAS.T:λ\cI-DD}, Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} brain glioma model when in combination with Cdk4^k06503.

(Read et al., 2009)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal 4 ventral denticle belt | germline clone | maternal effect

(Chen et al., 2003)

abdominal 5 ventral denticle belt | germline clone | maternal effect

(Chen et al., 2003)

abdominal 6 ventral denticle belt | germline clone | rescuable maternal effect

(Chen et al., 2003)

abdominal 7 ventral denticle belt | germline clone | rescuable maternal effect

(Chen et al., 2003)

abdominal 8 ventral denticle belt | germline clone | rescuable maternal effect

(Chen et al., 2003)

adult

(Meyer et al., 2000)

cell cycle

(Meyer et al., 2002)

embryo

(Meyer et al., 2002)

embryonic/first instar larval cuticle | germline clone | rescuable maternal effect

(Chen et al., 2003)

embryonic head | germline clone | rescuable maternal effect

(Chen et al., 2003)

fat body | third instar larval stage

(Baltzer et al., 2009)

mitochondrion | third instar larval stage

(Baltzer et al., 2009)

tracheal branch primordium | germline clone | maternal effect

(Chen et al., 2003)

tracheal dorsal trunk primordium | germline clone | maternal effect

(Chen et al., 2003)

wing

(Emmerich et al., 2004, Meyer et al., 2000)

Detailed Description

Statement

Reference

Fat body cells in homozygous Cdk4³ mutant larvae show reduced mitochondrial abundance compared to controls.

(Baltzer et al., 2009)

Cdk4³/Cdk4^k06503 mutant larval brains have normal gross morphology and glial cell numbers.

(Read et al., 2009)

Mutant flies show a reduction in body weight compared to wild type-controls.

(Datar et al., 2006)

Homozygotes are more sensitive to X rays than wild type or heterozygous sibling controls.

(Jaklevic et al., 2006)

87% of homozygous males survive to adulthood. Homozygous adult males weigh on average only 81% of the weight of control males. The average wing area of hemizygous males is only 94% that of wild type and the number of cells in the wing is 88% that of wild type. The cells in the wing of hemizygous males are slightly increased compared to wild type.

(Emmerich et al., 2004)

The progeny of Cdk4³ germ-line clone carrying mothers have segmentation defects. Most commonly the cuticles of such embryos have no denticle belt A5 and a gap in denticle belt A4. Segmentation defects are more severe in embryos also zygotically homozygous for Cdk4³, including reduction of the A2 and A8 denticle bands, fusion of the A6 and A7 bands, and head defects. These embryos also sometimes display other defects such as poor cuticle development and a dorsal hole. Paternally rescued Cdk4³ germ-line clone embryos have defective tracheal systems, generally having several disruptions in the main trunk and several branches. lateral secondary branches is also reduced.

(Chen et al., 2003)

only a few percent of eggs laid by Cdk4³ progress normally through the syncytial division cycles, while the other embryos are affected by division errors to a very variable extent. Most embryos display highly variable morphological abnormalities during later embryonic stages.

(Meyer et al., 2002)

Homozygous flies are slightly smaller, lighter and have a reduced number of cells in the wing (the only tissue where cell number was examined). Homozygous mutant clones are only about two-thirds of the area, on average, of wild-type sister clones.

(Meyer et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Cdk4³ has viable phenotype, non-enhanceable by CycB^unspecified

(Meyer et al., 2000)

Cdk4³ has viable phenotype, non-enhanceable by Cdk1^unspecified

(Meyer et al., 2000)

Cdk4³ has viable phenotype, non-enhanceable by CycA^unspecified

(Meyer et al., 2000)

Cdk4³ has viable phenotype, non-enhanceable by CycB3^unspecified

(Meyer et al., 2000)

Suppressed by

Statement

Reference

Cdk4³ has abnormal size phenotype, suppressible | partially by Scer\GAL4^da.PU/Hsap\CDK4^UAS.cDa

(Datar et al., 2006)

Cdk4³ has sterile | recessive | female phenotype, suppressible by Rbf¹¹

(Meyer et al., 2000)

Cdk4³ has sterile | recessive | female phenotype, suppressible by Rbf¹⁴

(Meyer et al., 2000)

NOT suppressed by

Statement

Reference

Cdk4³ has viable phenotype, non-suppressible by CycB^unspecified

(Meyer et al., 2000)

Cdk4³ has viable phenotype, non-suppressible by Cdk1^unspecified

(Meyer et al., 2000)

Cdk4³ has viable phenotype, non-suppressible by CycA^unspecified

(Meyer et al., 2000)

Cdk4³ has viable phenotype, non-suppressible by CycB3^unspecified

(Meyer et al., 2000)

Enhancer of

Statement

Reference

Cdk4³ is an enhancer of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Cdk4³ is an enhancer of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Cdk4³/Cdk4[+] is an enhancer of partially lethal - majority die | embryonic stage | maternal effect phenotype of hop²⁵

(Chen et al., 2003)

Cdk4³ is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

Cdk4³ is an enhancer of visible | adult stage phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

NOT Enhancer of

Statement

Reference

Cdk4³/Cdk4[+] is a non-enhancer of visible phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

Suppressor of

Statement

Reference

Cdk4^k06503/Cdk4³ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Cdk4^k06503/Cdk4³ is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Cdk4³ is a suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Cdk4³/Cdk4[+] is a suppressor of partially lethal - majority die | dominant | heat sensitive phenotype of hop^Tum

(Chen et al., 2003)

NOT Suppressor of

Statement

Reference

Cdk4³ is a non-suppressor of abnormal size | somatic clone phenotype of Hph^UAS.cFa, Scer\GAL4^Act5C.PP

(Frei and Edgar, 2004)

Cdk4³ is a non-suppressor of abnormal size | somatic clone phenotype of Hph^EP3200, Scer\GAL4^Act5C.PP

(Frei and Edgar, 2004)

Cdk4³/Cdk4[+] is a non-suppressor of visible phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

Cdk4³/Cdk4³ is a non-suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, mir-ban^EP3622

(Hipfner et al., 2002)

Cdk4³ is a non-suppressor of increased cell number | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Cdk4³ is a non-suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Other

Statement

Reference

Cdk4³, grp⁰⁶⁰³⁴ has lethal | recessive phenotype

(Jaklevic et al., 2006)

Cdk4³, CycE^unspecified has lethal | recessive phenotype

(Meyer et al., 2000)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Cdk4³ has mitochondrion | third instar larval stage phenotype, non-enhanceable by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Cdk4³ has fat body | third instar larval stage phenotype, non-enhanceable by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Enhancer of

Statement

Reference

Cdk4³ is an enhancer of eye phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Cdk4³/Cdk4[+] is an enhancer of abdominal 4 ventral denticle belt | maternal effect | germline clone phenotype of hop²⁵

(Chen et al., 2003)

Cdk4³/Cdk4[+] is an enhancer of abdominal 5 ventral denticle belt | maternal effect | germline clone phenotype of hop²⁵

(Chen et al., 2003)

Cdk4³/Cdk4[+] is an enhancer of abdominal 7 ventral denticle belt | maternal effect | germline clone phenotype of hop²⁵

(Chen et al., 2003)

NOT Enhancer of

Statement

Reference

Cdk4³/Cdk4[+] is a non-enhancer of wing phenotype of pyd^tam/pyd^ex147

(Djiane et al., 2011)

Cdk4³ is a non-enhancer of mitochondrion | third instar larval stage phenotype of Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Cdk4³ is a non-enhancer of fat body | third instar larval stage phenotype of Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Cdk4³/Cdk4[+] is a non-enhancer of eye phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

Suppressor of

Statement

Reference

Cdk4^k06503/Cdk4³ is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Cdk4^k06503/Cdk4³ is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Cdk4³/Cdk4[+] is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Rheb^AV4, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Cdk4³ is a suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

NOT Suppressor of

Statement

Reference

Cdk4³/Cdk4[+] is a non-suppressor of eye phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

Cdk4³/Cdk4³ is a non-suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, mir-ban^EP3622

(Hipfner et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Wings from pyd^ex147/pyd^tam flies are broader compared to control wings, and this phenotype is not modified in a Cdk4³/+ background.

(Djiane et al., 2011)

The reduced mitochondrial abundance seen in Cdk4³ or Ets97D⁶¹³/Df(3R)ro80b mutants alone is not enhanced in the double mutants.

(Baltzer et al., 2009)

Cdk4³/Cdk4^k06503 completely suppresses the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU.

(Read et al., 2009)

Overgrowth of Hph^EP3200; Scer\GAL4^Act5C.PP or Hph^Scer\UAS.cFa; Scer\GAL4^Act5C.PP somatic clones in third instar wing discs is unaffected by Cdk4³/Cdk4³.

(Frei and Edgar, 2004)

The weak segmentation defects of embryos derived from mothers carrying hop²⁵ germ-line clones are enhanced when these mothers are heterozygous for Cdk4³ : this is particularly noticeable for denticle belts A4 and A5 which are lost completely in such embryos. None of these embryos hatch. Survival to adulthood of hop^Tum/+ females raised at 29^oC is restored to 96% wild-type levels by heterozygosity for Cdk4³.

(Chen et al., 2003)

Expression of ptc^Scer\UAS.cCa under the control of Scer\GAL4^Act5C.PP does not significantly reduce the size of clones induced in the wing disc in a Cdk4³ mutant background. Expression of ci^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP does not significantly increase the size of clones induced in the wing disc in a Cdk4³ mutant background.

(Duman-Scheel et al., 2002)

The wing overgrowth phenotype caused by expression of ban^EP3622 under the control of Scer\GAL4^Bx-MS1096 is not altered by Cdk4³/Cdk4³. The eye phenotype caused by expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev is not altered by Cdk4³/+.

(Hipfner et al., 2002)

When tkv^{Q253D.Scer\UAS.cNc} is driven by Scer\GAL4^Act5C.PP in a Cdk4³ background, no suppression of the proliferation phenotype is seen.

(Martin-Castellanos and Edgar, 2002)

Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the eye under the control of Scer\GAL4^ey.PH results in a much smaller eye than normal. Addition of one copy of Cdk4³ enhances the phenotype.

(Tapon et al., 2001)

The addition of CycA^unspecified, CycB^unspecified, CycB3^unspecified or cdc2^unspecified have no effect on survival of Cdk4³ homozygotes.

(Meyer et al., 2000)

Xenogenetic Interactions

Statement

Reference

Cdk4³ enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

(Gupta et al., 2008)

Expression of Hsap\CDK4^Scer\UAS.cDa under the control of Scer\GAL4^da.G32 partially suppresses the reduced body weight of Cdk4³ flies.

(Datar et al., 2006)

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Rheb^AV4. This enhancement is blocked in a Cdk4³/+ background.

(Khurana et al., 2006)

Complementation and Rescue Data

Rescued by

Cdk4³ is rescued by Cdk4^hs.PC

(Chen et al., 2003)

Cdk4³ is rescued by Scer\GAL4^da.G32/Cdk4^UAS.cMa

(Meyer et al., 2000)

Not rescued by

Cdk4³ is not rescued by Scer\GAL4^da.G32/Cdk4^{D175N.UAS.Tag:MYC}

(Meyer et al., 2000)

Comments

The addition of Cdk4^Scer\UAS.cMa driven by Scer\GAL4^da.G32 rescues the weight, body and cell size phenotypes seen in Cdk4³ flies. Whilst similar expression of Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} has no effect. Heterozygosity in CycE^unspecified is tolerated in Cdk4³ heterozygotes, but leads to complete lethality in Cdk4³ homozygotes. Cdk4³ homozygotes also heterozygous for Rbf¹¹ (and probably Rbf¹⁴) are found to be significantly more fertile than Cdk4³ homozygotes alone.

(Meyer et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6644

w^*; Cdk4³/CyO, P{ftz-lacB}E3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

(Mukherjee et al., 2006)

Cdk4³

(Romero-Pozuelo et al., 2017, Khurana et al., 2006)

cdk4³

(Manansala et al., 2013, Djiane et al., 2011, Baltzer et al., 2009, Gupta et al., 2008, Datar et al., 2006, Jaklevic et al., 2006, Scott et al., 2004, Duman-Scheel et al., 2002, Hipfner et al., 2002, Tapon et al., 2001)

dCdk4³

(Read et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (21)

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