Cdk4/6, l(2)05428, l(2)s4639, 8-6, l(2)k06503
Gene model reviewed during 5.50
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
1.2 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cdk4 using the Feature Mapper tool.
Cdk4 transcripts are detected at all stages of development on northern blots. They are found to be ubiquitous in early embryos by in situ hybridization. They are also present at a low level throughout the embryo at later stages of embryogenesis with stronger signals observed in internal tissues of germ band retracted embryos in a pattern correlated with endoreduplicaiton in these tissues.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Cdk4 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Cdk4/6 CG5072
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an decrease, an increase in the proportion of S phase cells, an increase in cell size, in the proportion of G2/M phase cells and spindle abnormalities (branched spindles) are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages. This phenotype is not seen in Kc167 cells.
Cdk4 has a role in the modulation of growth rates and is dispensable for cell cycle progression.
Gene in D.melanogaster encoding product related (by sequence comparison) to the serine-threonine protein kinases of mammals. Isolated from Drosophila clones obtained with mammalian probes.