FlyBase Allele Report: Dmel\Cdk4[k06503]

General Information

Symbol

Dmel\Cdk4^k06503

Species

D. melanogaster

Name

FlyBase ID

FBal0064432

Feature type

allele

Associated gene

Dmel\Cdk4

Associated Insertion(s)

P{lacW}Cdk4^k06503

Carried in Construct

Also Known As

l(2)k06503

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Cdk4^k06503

Cytology

Description

The P{lacW} insertion is in the second exon of the translated region of the Cdk4 gene.

(Meyer et al., 2000)

P{lacW} insertion in the third intron of Cdk4.

(Misra, 2000.8.25)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:16,583,961..16,583,961 [+]

(Sainz de la Maza et al., 2022, Loren et al., 2003, Meyer et al., 2000, Misra, 2000.8.25, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Heterozygous Cdk4^k06503 ameliorates the neoplasia seen in the btl::Egfr^{Scer\UAS.T:λ\cI-DD}, Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} brain glioma model when in combination with Cdk4³.

(Read et al., 2009)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Cdk4³/Cdk4^k06503 mutant larval brains have normal gross morphology and glial cell numbers.

(Read et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Cdk4^k06503 is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

Cdk4^k06503/Cdk4³ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Cdk4^k06503/Cdk4³ is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

NOT Suppressor of

Statement

Reference

Cdk4^k06503/Cdk4[+] is a non-suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Cdk4^k06503 is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Cdk4^k06503/Cdk4[+] is a non-enhancer of wing phenotype of pyd^tam/pyd^ex147

(Djiane et al., 2011)

Cdk4^k06503 is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

Cdk4^k06503/Cdk4³ is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Cdk4^k06503/Cdk4³ is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

NOT Suppressor of

Statement

Reference

Cdk4^k06503/Cdk4[+] is a non-suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Cdk4^k06503/Cdk4[+] is a non-suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Cdk4^k06503 is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Wings from pyd^ex147/pyd^tam flies are broader compared to control wings, and this phenotype is not modified in a Cdk4^k06503/+ background.

(Djiane et al., 2011)

Cdk4³/Cdk4^k06503 completely suppresses the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU.

(Read et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10629

y¹ w^67c23; P{lacW}Cdk4^k06503/CyO

Kyoto

111198

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Cdk4^k06503 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Cdk4/6^k06503

Cdk4⁰⁶⁵⁰³

(Morris et al., 2006)

Cdk4^k06503

(Sainz de la Maza et al., 2022, Tanaka-Matakatsu et al., 2007, Loren et al., 2003)

cdk4^k06503

(Djiane et al., 2011)

dCdk4^k06503

(Read et al., 2009)

k06503

(Mukherjee et al., 2006)

l(2)k06503

(Loren et al., 2001, Misra, 2000.8.25, Spradling et al., 1999)

l(2)k06503^k06503

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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