FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Mps1
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General Information
Symbol
Dmel\Mps1
Species
D. melanogaster
Name
Monopolar spindle 1
Annotation Symbol
CG7643
Feature Type
protein_coding_gene
FlyBase ID
FBgn0000063
Gene Model Status
Current
Stock Availability
16 publicly available
Enzyme Name (EC)
dual-specificity kinase (2.7.12.1)
Gene Summary
Monopolar spindle 1 (Mps1) encodes a conserved protein kinase that is associated with kinetochores and is required for the mitotic and meiotic spindle assembly checkpoints. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)
All Summaries
Also Known As

ald, altered disjunction, dMps1

Key Links
Genomic Location
Cytogenetic map
90C1-90C1
Sequence location
Recombination map
3-61
RefSeq locus
NT_033777 REGION:17671271..17673828
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (23 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein serine/threonine/tyrosine kinase activity
inferred from direct assay
(Cunha-Silva et al., 2020)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441
(InterPro Project Members, 2004-)
enables protein kinase activity
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271
(InterPro Project Members, 2004-)
enables protein serine/threonine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000540737
(GO Reference Genome Project, 2011-)
enables protein serine/threonine/tyrosine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(GO Reference Genome Project, 2011-)
Biological Process (15 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in achiasmate meiosis I
inferred from mutant phenotype
(Gilliland et al., 2005)
involved_in distributive segregation
inferred from mutant phenotype
(Whyte et al., 1993)
inferred from genetic interaction with FLYBASE:Axs; FB:FBgn0000152
(Whyte et al., 1993)
involved_in female meiosis chromosome segregation
inferred from mutant phenotype
(Gilliland et al., 2005, O'Tousa, 1982)
involved_in female meiotic nuclear division
inferred from mutant phenotype
(Gilliland et al., 2007)
involved_in male germ-line stem cell asymmetric division
inferred from genetic interaction with FLYBASE:Mgtor; FB:FBgn0013756
(Liu et al., 2015)
involved_in meiotic cell cycle
inferred from mutant phenotype
(Page et al., 2007)
involved_in meiotic metaphase I homologous chromosome alignment
inferred from mutant phenotype
(Gilliland et al., 2005)
involved_in meiotic spindle assembly checkpoint signaling
inferred from mutant phenotype
(Gilliland et al., 2007)
involved_in meiotic spindle checkpoint signaling
inferred from mutant phenotype
(Gilliland et al., 2005)
involved_in mitotic spindle assembly checkpoint signaling
inferred from direct assay
(Cunha-Silva et al., 2020)
inferred from mutant phenotype
(Fischer et al., 2004)
involved_in negative regulation of sister chromatid segregation
inferred from mutant phenotype
(Althoff et al., 2012)
involved_in positive regulation of protein localization to kinetochore
inferred from direct assay
(Cunha-Silva et al., 2020)
involved_in response to hypoxia
inferred from mutant phenotype
(Fischer et al., 2004)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in chromosome segregation
inferred from biological aspect of ancestor with PANTHER:PTN000540737
(GO Reference Genome Project, 2011-)
involved_in meiotic spindle assembly checkpoint signaling
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(GO Reference Genome Project, 2011-)
involved_in mitotic spindle assembly checkpoint signaling
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(GO Reference Genome Project, 2011-)
involved_in protein localization to kinetochore
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(GO Reference Genome Project, 2011-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
(Althoff et al., 2012)
located_in kinetochore
inferred from direct assay
(Althoff et al., 2012)
part_of outer kinetochore
inferred from direct assay
(Gilliland et al., 2007)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in kinetochore
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000540737
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
protein serine/threonine/tyrosine kinase activity
(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (2.7.12.1)
(2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (2.7.12.1)
(3) L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+) (2.7.12.1)
protein serine/threonine kinase activity
Summaries
Gene Snapshot
Monopolar spindle 1 (Mps1) encodes a conserved protein kinase that is associated with kinetochores and is required for the mitotic and meiotic spindle assembly checkpoints. [Date last reviewed: 2018-09-06]
Gene Group (FlyBase)
UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -
This group comprises conventional protein kinase domain kinases that do not classify under other groups in FlyBase.
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ald: altered disjunction (A.T.C. Carpenter)
Homozygous females display elevated levels of non-disjunction of X and fourth chromosomes (9.5 and 6.0% respectively); double exceptions are predominantly XX;0 and 0;44, products expected from nonhomologous disjunction; behavior of large autosomes nearly normal. Exchange frequencies normal, and sex-chromosome exchange tetrads contribute to exceptional products.
Summary (Interactive Fly)

kinase - regulation of mitotic spindle checkpoint - required for cell cycle arrest in response to hypoxia - ensures the proper segregation of achiasmatic chromosomes during meiosis - required for cell cycle arrest of the three haploid products of female meiosis that are not used as the female pronucleus

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Mps1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A0A0B4KG66)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.44

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mps1-RA
FBtr0083478
NM_142387
2113
630
Mps1-RB
FBtr0331348
NM_001275748
2141
672
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Mps1-PA
FBpp0082911
69.8
630
8.44
Q9VEH1
NP_650644
AAF55450
Mps1-PB
FBpp0303766
74.3
672
7.59
A0A0B4KG66
NP_001262677
AGB96057
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mps1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.17

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
(Gilliland et al., 2007)
oogenesis
oocyte
(Gilliland et al., 2007)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval brain
(Herriott et al., 2012)
Additional Descriptive Data

ald protein localizes to the outer kinetochore in meiotic and mitotic nuclei. It is also observed in cytoplasmic filaments in prometaphase oocytes.

(Gilliland et al., 2007)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
(Althoff et al., 2012)
located_in kinetochore
inferred from direct assay
(Althoff et al., 2012)
part_of outer kinetochore
inferred from direct assay
(Gilliland et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mps1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 16 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 29 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mps1
Transgenic constructs containing regulatory region of Mps1
Aberrations (Deficiencies and Duplications) ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of P-stage | recessive
lethal - all die during embryonic stage
partially lethal - majority die
viable
viable (with Df(3R)ED5780)
Sterility
Allele
female semi-sterile, with Scer\GAL4VP16.nanos.UTR, Scer\GAL4VP16.otu, Scer\GAL4nanos.PG
fertile
fertile (with Df(3R)ED5780)
Other Phenotypes
Allele
abnormal meiotic cell cycle
abnormal meiotic cell cycle (with Df(3R)AN6)
abnormal meiotic cell cycle (with Mps1C3)
abnormal meiotic cell cycle (with Mps1Exc23)
abnormal meiotic cell cycle | female
abnormal meiotic cell cycle | female (with Df(3R)ED5780)
abnormal meiotic cell cycle | female | germline clone
abnormal meiotic cell cycle | oogenesis, with Scer\GAL4VP16.mat.αTub67C
abnormal meiotic cell cycle | oogenesis (with Mps1ald-1)
abnormal meiotic cell cycle | oogenesis (with Mps1C3)
abnormal mitotic cell cycle | embryonic stage 5, with Scer\GAL4VP16.mat.αTub67C
abnormal mitotic cell cycle | embryonic stage 5 | conditional, with Scer\GAL4VP16.mat.αTub67C
some die during embryonic stage
some die during pharate adult stage | recessive
Phenotype manifest in
Allele
anaphase & condensed nuclear chromosome | germ-line clone
chromosome & oocyte (with Mps1ald-1)
chromosome & oocyte (with Mps1C3)
eye
kinetochore | embryonic stage 5, with Scer\GAL4VP16.mat.αTub67C
meiosis & nuclear chromosome
meiosis & nuclear chromosome (with Df(3R)ED5780)
mitotic cell cycle | germline clone
nucleus | germline clone
ommatidium
oocyte
oocyte, with Scer\GAL4VP16.mat.αTub67C
oocyte (with Df(3R)AN6)
oocyte (with Mps1ald-1)
oocyte (with Mps1C3)
oocyte (with Mps1Exc23)
polar body nucleus | germline clone
spindle microtubule & oocyte (with Mps1ald-1)
spindle microtubule & oocyte (with Mps1C3)
spindle pole & oocyte (with Mps1ald-1)
spindle pole & oocyte (with Mps1C3)
wing
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (32)
Hsap\TTK
11 of 14
Yes
Yes
1  
Hsap\CAMK1D
1 of 14
No
No
1  
Hsap\CDC7
1 of 14
No
No
Hsap\CDC42BPG
1 of 14
No
No
Hsap\CDK2
1 of 14
No
No
1  
Hsap\CDK6
1 of 14
No
No
1  
Hsap\CDK15
1 of 14
No
No
Hsap\CDK20
1 of 14
No
No
Hsap\CDKL3
1 of 14
No
No
1  
Hsap\CHEK2
1 of 14
No
No
Hsap\DYRK2
1 of 14
No
No
Hsap\DYRK3
1 of 14
No
No
1  
Hsap\DYRK4
1 of 14
No
No
1  
Hsap\GAK
1 of 14
No
No
2  
Hsap\MAP3K4
1 of 14
No
No
Hsap\MAP3K19
1 of 14
No
No
Hsap\MAPK7
1 of 14
No
No
Hsap\NEK6
1 of 14
No
No
Hsap\NEK7
1 of 14
No
No
Hsap\OBSCN
1 of 14
No
No
Hsap\PAK1
1 of 14
No
No
Hsap\PAK2
1 of 14
No
No
Hsap\PAK3
1 of 14
No
No
Hsap\PHKG2
1 of 14
No
No
1  
Hsap\PLK4
1 of 14
No
No
Hsap\ROCK1
1 of 14
No
No
Hsap\ROCK2
1 of 14
No
No
1  
Hsap\SLK
1 of 14
No
No
6  
Hsap\SNRK
1 of 14
No
No
1  
Hsap\STK10
1 of 14
No
No
Hsap\STK36
1 of 14
No
No
1  
Hsap\UHMK1
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (28)
Rnor\Ttk
10 of 14
Yes
Yes
Rnor\Camk1d
1 of 14
No
No
Rnor\Cdc7
1 of 14
No
No
Rnor\Cdc42bpg
1 of 14
No
No
Rnor\Cdk2
1 of 14
No
No
Rnor\Cdk6
1 of 14
No
No
Rnor\Cdkl3
1 of 14
No
No
Rnor\Cit
1 of 14
No
No
Rnor\Dyrk2
1 of 14
No
No
Rnor\Dyrk3
1 of 14
No
No
Rnor\LOC690235
1 of 14
No
No
Rnor\Map3k4
1 of 14
No
No
Rnor\Map3k19
1 of 14
No
No
Rnor\Mok
1 of 14
No
No
Rnor\Nek6
1 of 14
No
No
Rnor\Nek7
1 of 14
No
No
Rnor\Pak1
1 of 14
No
No
Rnor\Pak2
1 of 14
No
No
Rnor\Phkg1
1 of 14
No
No
Rnor\Phkg2
1 of 14
No
No
Rnor\Plk4
1 of 14
No
No
Rnor\Plk5
1 of 14
No
No
Rnor\Rock1
1 of 14
No
No
Rnor\Rock2
1 of 14
No
No
Rnor\Slk
1 of 14
No
No
Rnor\Snrk
1 of 14
No
No
Rnor\Stk36
1 of 14
No
No
Rnor\Uhmk1
1 of 14
No
Yes
Mus musculus (laboratory mouse) (25)
Mmus\Ttk
10 of 14
Yes
Yes
Mmus\Camk1d
1 of 14
No
No
Mmus\Camk4
1 of 14
No
No
Mmus\Cdc7
1 of 14
No
No
Mmus\Cdc42bpg
1 of 14
No
No
Mmus\Cdk6
1 of 14
No
No
Mmus\Cdk15
1 of 14
No
No
Mmus\Cdkl3
1 of 14
No
No
Mmus\Dyrk2
1 of 14
No
No
Mmus\Dyrk3
1 of 14
No
No
Mmus\Map3k4
1 of 14
No
No
Mmus\Map3k19
1 of 14
No
Yes
Mmus\Nek6
1 of 14
No
No
Mmus\Pak2
1 of 14
No
No
Mmus\Phkg2
1 of 14
No
No
Mmus\Plk2
1 of 14
No
No
Mmus\Plk4
1 of 14
No
No
Mmus\Plk5
1 of 14
No
No
Mmus\Rock1
1 of 14
No
No
Mmus\Rock2
1 of 14
No
No
Mmus\Slk
1 of 14
No
No
Mmus\Smok2a
1 of 14
No
No
Mmus\Snrk
1 of 14
No
No
Mmus\Stk36
1 of 14
No
No
Mmus\Uhmk1
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (18)
Xtro\ttk
7 of 13
Yes
Yes
Xtro\camkk1
1 of 13
No
No
Xtro\cdk1
1 of 13
No
No
Xtro\cdk15
1 of 13
No
No
Xtro\cit
1 of 13
No
No
Xtro\dclk3
1 of 13
No
No
Xtro\dyrk2
1 of 13
No
No
Xtro\dyrk3
1 of 13
No
No
Xtro\LOC100486333
1 of 13
No
No
Xtro\map3k10
1 of 13
No
No
Xtro\map3k19
1 of 13
No
No
Xtro\mapk7
1 of 13
No
No
Xtro\mark4
1 of 13
No
No
Xtro\pak2
1 of 13
No
No
Xtro\pak3
1 of 13
No
No
Xtro\rock1
1 of 13
No
No
Xtro\snrk
1 of 13
No
No
Xtro\tent5c
1 of 13
No
No
Danio rerio (Zebrafish) (31)
Drer\ttk
10 of 14
Yes
Yes
Drer\cdc7
1 of 14
No
No
Drer\cdk2
1 of 14
No
No
Drer\cdk6
1 of 14
No
No
Drer\cdk20
1 of 14
No
No
Drer\cdk21
1 of 14
No
No
Drer\dyrk2
1 of 14
No
No
Drer\dyrk3
1 of 14
No
No
Drer\dyrk4
1 of 14
No
No
Drer\map3k4
1 of 14
No
No
Drer\mapk7
1 of 14
No
No
Drer\pak2a
1 of 14
No
No
Drer\pak2b
1 of 14
No
No
Drer\pdpk1b
1 of 14
No
No
Drer\phkg1a
1 of 14
No
No
Drer\phkg1b
1 of 14
No
No
Drer\phkg2
1 of 14
No
No
Drer\pimr117
1 of 14
No
Yes
Drer\pimr118
1 of 14
No
Yes
Drer\pimr119
1 of 14
No
Yes
Drer\pimr120
1 of 14
No
Yes
Drer\pimr210
1 of 14
No
Yes
Drer\pimr212
1 of 14
No
Yes
Drer\plk2b
1 of 14
No
No
Drer\plk3
1 of 14
No
No
Drer\plk4
1 of 14
No
No
Drer\rock1
1 of 14
No
No
Drer\slka
1 of 14
No
No
Drer\snrkb
1 of 14
No
No
Drer\stk36
1 of 14
No
No
Drer\uhmk1
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (10)
Cele\cdc-7
1 of 14
Yes
No
Cele\cdk-2
1 of 14
Yes
No
Cele\gakh-1
1 of 14
Yes
No
Cele\kin-3
1 of 14
Yes
No
Cele\mbk-1
1 of 14
Yes
No
Cele\mbk-2
1 of 14
Yes
No
Cele\mlck-1
1 of 14
Yes
No
Cele\mnk-1
1 of 14
Yes
No
Cele\nekl-3
1 of 14
Yes
No
Cele\ZK524.4
1 of 14
Yes
No
Anopheles gambiae (African malaria mosquito) (11)
Agam\AgaP_AGAP002075
11 of 12
Yes
Yes
Agam\AgaP_AGAP000028
1 of 12
No
No
Agam\AgaP_AGAP002110
1 of 12
No
No
Agam\AgaP_AGAP002250
1 of 12
No
No
Agam\AgaP_AGAP002647
1 of 12
No
No
Agam\AgaP_AGAP002986
1 of 12
No
No
Agam\AgaP_AGAP005729
1 of 12
No
No
Agam\AgaP_AGAP008241
1 of 12
No
No
Agam\AgaP_AGAP008341
1 of 12
No
No
Agam\AgaP_AGAP008541
1 of 12
No
No
Agam\AgaP_AGAP009459
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (32)
Atha\PPK1
8 of 13
Yes
Yes
Atha\AT1G03740
1 of 13
No
No
Atha\AT1G71530
1 of 13
No
No
Atha\AT2G34290
1 of 13
No
Yes
Atha\AT3G61960
1 of 13
No
No
Atha\AT4G16970
1 of 13
No
No
Atha\CAK1AT
1 of 13
No
Yes
Atha\CDC2
1 of 13
No
No
Atha\CDKB2;1
1 of 13
No
No
Atha\CDKB2;2
1 of 13
No
No
Atha\CIPK3
1 of 13
No
No
Atha\CIPK12
1 of 13
No
No
Atha\CIPK18
1 of 13
No
Yes
Atha\CIPK19
1 of 13
No
No
Atha\CIPK26
1 of 13
No
No
Atha\CPK17
1 of 13
No
No
Atha\CPK34
1 of 13
No
No
Atha\FU
1 of 13
No
No
Atha\MAP3KA
1 of 13
No
No
Atha\MAPKKK5
1 of 13
No
No
Atha\MAPKKK6
1 of 13
No
No
Atha\MAPKKK7
1 of 13
No
No
Atha\MAPKKK9
1 of 13
No
No
Atha\MEKK1
1 of 13
No
No
Atha\MEKK3
1 of 13
No
No
Atha\MHK
1 of 13
No
No
Atha\MKK2
1 of 13
No
No
Atha\MKK4
1 of 13
No
No
Atha\MKK5
1 of 13
No
No
Atha\NP1
1 of 13
No
No
Atha\TSL
1 of 13
No
No
Atha\YDA
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (16)
Scer\MPS1
10 of 13
Yes
Yes
Scer\CDC7
1 of 13
No
No
Scer\CDC28
1 of 13
No
No
Scer\CKA2
1 of 13
No
No
Scer\CMK1
1 of 13
No
No
Scer\GIN4
1 of 13
No
No
Scer\HOG1
1 of 13
No
No
Scer\KIC1
1 of 13
No
No
Scer\KSS1
1 of 13
No
No
Scer\PHO85
1 of 13
No
No
Scer\PKH1
1 of 13
No
No
Scer\PKH2
1 of 13
No
No
Scer\PKH3
1 of 13
No
No
Scer\SLT2
1 of 13
No
No
Scer\SSK2
1 of 13
No
No
Scer\YAK1
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (10)
Spom\mph1
9 of 12
Yes
Yes
Spom\cdk9
1 of 12
No
No
Spom\cka1
1 of 12
No
No
Spom\hsk1
1 of 12
No
No
Spom\pef1
1 of 12
No
No
Spom\pom1
1 of 12
No
No
Spom\ppk15
1 of 12
No
No
Spom\shk1
1 of 12
No
No
Spom\spo4
1 of 12
No
No
Spom\sty1
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Mps1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (14)
Tlk
2 of 13
Aduk
1 of 13
aux
1 of 13
CaMKII
1 of 13
Cdc7
1 of 13
Cdk1
1 of 13
Cdk4
1 of 13
CG5790
1 of 13
Dyrk3
1 of 13
Mekk1
1 of 13
Nek2
1 of 13
Pak
1 of 13
PhKγ
1 of 13
Snrk
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Evidence
    References
    Mps1GL00184
    model of  cancer
    CEA
    (Brás et al., 2021)
    Mps1HMS01546
    model of  cancer
    CEA
    (Brás et al., 2021)
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    Mps1GL00184
    exacerbates  cancer
    modeled by RafUAS.F179
    (Brás et al., 2021)
    modeled by NGD14477
    (Brás et al., 2021)
    model of  cancer
    is exacerbated by NGD14477
    (Brás et al., 2021)
    is exacerbated by RafUAS.F179
    (Brás et al., 2021)
    Mps1HMS01546
    exacerbates  cancer
    modeled by RafUAS.F179
    (Brás et al., 2021)
    modeled by NGD14477
    (Brás et al., 2021)
    model of  cancer
    is exacerbated by NGD14477
    (Brás et al., 2021)
    is exacerbated by RafUAS.F179
    (Brás et al., 2021)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TTK; TTK protein kinase
    11 of 14
    604092
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Mps1
        suppressible
        alt
        (Gilliland et al., 2005)
        Mps1
        suppressible
        mad2
        (Althoff et al., 2012)
        Mps1|mad2
        suppressible
        vtd
        (Althoff et al., 2012)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Mgtor
        suppressible
        Mps1
        (Liu et al., 2015)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-61
        Cytogenetic map
        90C1-90C1
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        90C1-90C1
        Limits computationally determined from genome sequence between P{PZ}l(3)0788207882 and P{EP}cpoEP3679
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        90C1-90C1
        (Gilliland et al., 2005)
        Determined by deficiency mapping followed by SNP mapping.
        (Gilliland et al., 2005)
        Experimentally Determined Recombination Data
        Location

        3-61

        (FlyBase, 2016)

        3-59.9

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (16)
        Genomic Clones (30)
        cDNA Clones (17)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Gilliland et al., 2007)
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             

            • New stable cell line derived from S2-DGRC : Multiple stable S2 cell lines were created involving Mps1 and mutated versions of aurA (designated AAK) to study the involvement of kinetochores in chromosome segregation. Cell lines expressing GFP-His2B and mCherry-α-tubulin were also created.

              (Leça et al., 2025)
            Other Comments

            The rate of meiotic errors in ald mutant females is linked to an increased likelihood of premature release of sister chromatid cohesion, rather than just a shortening of prometaphase duration.

            (Gilliland et al., 2007)

            dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of ald on the spindle checkpoint.

            (Muller et al., 2006)

            CG31251- CG31360+ CG31249- Overall orientation not stated: CG14322+ CG7523- ald+ CG18212- CG7655+

            (Gilliland et al., 2005)

            Cytological analysis shows that ald plays a role in the maintenance of the chiasmate homolog associations during meiotic metaphase I.

            (Gilliland et al., 2005)

            When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, decrease in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells and spindle abnormalities are seen.

            (Bettencourt-Dias et al., 2004)

            RNAi experiments in S2 cells causes no discernable phenotype. Over-expression results in cells displaying abnormal anaphases, with chromatin stretched over the central spindle.

            (Faria et al., 2004)

            ald is required for the mitotic spindle checkpoint and is required for the metaphase arrest that results in response to hypoxic conditions.

            (Fischer et al., 2004)

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            ald kinase plays a role in centrosome duplication and the mitotic spindle checkpoint.

            (Lehner, 2003)

            Mutant females show an increased frequency of nondisjunction of the X and 4th chromosomes.

            Relationship to Other Genes
            Source for database merge of

            Source for merge of: ald Mps1

            (Gilliland et al., 2005)
            Additional comments
            Nomenclature History
            Source for database identify of

            Source for identity of: Mps1 CG7643

            (Faria, 2004.2.23)

            Source for identity of: Mps1 ald

            (FlyBase, 2009-)
            Nomenclature comments

            The gene symbol "ald" has been changed to "Mps1" to reflect published usage and in order to reduce confusion with the "Ald" ("Aldolase") gene symbol, from which it differed only by case.

            (FlyBase, 2009-)
            Etymology
            Synonyms and Secondary IDs (13)
            Reported As
            Symbol Synonym
            Ald
            (Resende et al., 2018)
            Ald/Mps1
            (Gilliland et al., 2009)
            CG7643
            (Meadows, 2017.1.11, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Gilliland et al., 2005, Gilliland et al., 2005, Bettencourt-Dias et al., 2004, Fischer et al., 2004, Stanyon et al., 2004, Asha et al., 2003, Manning et al., 2002)
            MPS1
            (Leça et al., 2025)
            Mps1
            (Shapiro et al., 2025, Miller et al., 2023, Cunha-Silva et al., 2020, Port et al., 2020, Oliveira et al., 2019, Gene Disruption Project members, 2018-, Levis, 2018.8.30, Moura et al., 2017, Derive et al., 2015, Conde et al., 2013, Emre et al., 2011, Althoff et al., 2009, Gilliland et al., 2009, Gilliland et al., 2009, Wen et al., 2008, Lehner et al., 2007, Pandey et al., 2007, Schuh et al., 2007, Schuh et al., 2007, Muller et al., 2006, Buffin et al., 2005, Gilliland et al., 2005, Hacker, 2005.4.28, Faria, 2004.2.23, Fischer et al., 2004, Fisk and Winey, 2004, Manning et al., 2002)
            Msp1
            (Althoff et al., 2012)
            TKK
            (Morrison et al., 2000)
            ald
            (Miller et al., 2023, Vicars et al., 2021, Hughes et al., 2018, Moura et al., 2017, Das et al., 2016, Sopko et al., 2014, Conde et al., 2013, de Celis et al., 2013.9.11, Japanese National Institute of Genetics, 2012.5.21, Reis et al., 2011, Anderson et al., 2009, Hughes et al., 2009, Wu et al., 2008, Georlette et al., 2007, Gilliland et al., 2007, Gilliland et al., 2007, Page et al., 2007, Hawley and Gilliland, 2006, Whyte et al., 1993)
            dMps1
            (Faria et al., 2005, Faria et al., 2004)
            mps1
            (Brás et al., 2021, Wang et al., 2019, Hughes et al., 2018, Resende et al., 2018, Silva et al., 2018, Liu et al., 2015, Batiha and Swan, 2012, Lince-Faria et al., 2009, Gilliland et al., 2007, Gilliland et al., 2007, Page et al., 2007, Hawley and Gilliland, 2006)
            Name Synonyms
            Monopolar spindle 1
            altered disjunction
            (Page et al., 2007)
            monopolar spindles 1
            (Gilliland et al., 2007)
            Secondary FlyBase IDs
            • FBgn0038534
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 41 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            References (101)