Cells were treated with MG132 for one hour, then two hours with colchicine, to induce the formation of the mitotic checkpoint complex (MCC).

The BubR1-ald interaction is not affected by depletion of Ndc80 and Nuf2, suggesting that the interaction might occur outside kinetochores.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Cells were treated with MG132 for one hour, then two hours with colchicine, to induce the formation of the mitotic checkpoint complex (MCC).

The BubR1-ald interaction is not affected by depletion of Ndc80 and Nuf2, suggesting that the interaction might occur outside kinetochores.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.