Expression of ykiS111A.S250A.Scer\UAS.T:SV5\V5 enhances the overgrowth seen in wing disc clones expressing MadScer\UAS.T:Zzzz\FLAG and tkvQ253D.Scer\UAS.cNc under the control of Scer\GAL4Scer\FRT.Act5C. The phenotype seen in stronger than is seen when ykiS111A.S250A.Scer\UAS.T:SV5\V5 is expressed alone.
A ykiB5 mutant background suppresses the overgrowth seen when tkvQ253D.Scer\UAS.cNc is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C. As in ykiB5 mutants alone, very few clones are recoverable.
Co-expression of tkvQ253D.Scer\UAS.cNc and MadScer\UAS.T:Zzzz\FLAG does not suppress the growth and survival defects seen when brkScer\UAS.cJa is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C.
Expression of ykiS168A.Scer\UAS.T:SV5\V5 suppresses the small eye phenotype seen when brkScer\UAS.cJa is expressed under the control of Scer\GAL4GMR.PF. When tkvQ253D.Scer\UAS.cNc is additionally expressed overgrown eyes are seen.
Expression of ykiS168A.Scer\UAS.T:SV5\V5 partially suppresses the underproliferation seen when brkScer\UAS.cJa is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C. Co-expression of tkvQ253D.Scer\UAS.cNc suppresses the growth defects further, producing some clone overgrowth, whilst the addition of MadScer\UAS.T:Zzzz\FLAG increases clone overgrowth even further.
The addition of RbfScer\UAS.cDa to tkvQ253D.Scer\UAS.cNc (driven by Scer\GAL4Act5C.PP) suppresses the proliferation and wing phenotypes seen in tkvQ253D.Scer\UAS.cNc, Scer\GAL4Act5C.PP alone. These animals eclose at normal rates and show at most very mild morphological defects. The addition of Pi3K21BScer\UAS.Δ.T:Ivir\HA1 blocks the growth and proliferation effects tkvQ253D.Scer\UAS.cNc, Scer\GAL4Act5C.PP clones. They contain as few cells as Pi3K21BScer\UAS.Δ.T:Ivir\HA1 (Scer\GAL4Act5C.PP) cells alone, but those cells are slightly larger. When tkvQ253D.Scer\UAS.cNc is driven by Scer\GAL4Act5C.PP in a biomb-3198 or Cdk43 background, no suppression of the proliferation phenotype is seen. When tkvQ253D.Scer\UAS.cNc is driven by Scer\GAL4Act5C.PP in a vg83b27-R background, complete suppression of the proliferation phenotype is seen.