No tracheal defects are apparent in SelDk11320 mutants.
Heterozygous flies develop into normal-appearing, viable adults which can mate and give progeny. Heterozygous males do not show a significantly different mean life span than controls. Heterozygous male adults show hypersensitivity to paraquat and increased sensitivity to hydrogen peroxide compared to controls.
Homozygotes die during the third larval instar stage. Mutant brains do not have optic lobes. The pattern of BrdU incorporation indicates that proliferation is significantly reduced in the brain hemispheres and ventral ganglion compared to wild type. The percentage of cells incorporating BrdU in homozygous imaginal discs (less than 10%) is reduced compared to wild-type (34%).
Homozygous larvae die at around days 8 and 9 AEL without entering pupation. Dissected mutants exhibit an aberrant imaginal disc morphology characterised by a reduction in mass of discs and disorganisation of disc cells where no folding or patterning can be detected. Apoptotic cells are observed in these small and abnormal mutant discs. Larval brain hemispheres are smaller than wild type. Mutants transformed with the full-length cDNA UAS construct expressed from Scer\GAL469B show complete reversion of lethality and disc phenotype. Mitotic clones cause reduced cell viability, smaller cell and clone size and clones in the wing stop wing vein differentiation. Non-autonomous effects, such as abnormal differentiation of wild type cells surrounding the clones, are also seen: change in orientation of surrounding wild type trichomes and extra veins.
Mutants exhibit visible disc abnormalities: very small or no discs. Clones induced in wild type discs are abnormal: clone are small and densely packed with contours that appear smooth defining sharp borders. Clones on the wing can affect wing veins and have non-autonomous effects on neighbouring cells.