FlyBase Allele Report: Dmel\Rho1[GMR.PH]

General Information

Symbol

Dmel\Rho1^GMR.PH

Species

D. melanogaster

Name

glass multimer reporter construct of Hariharan

FlyBase ID

FBal0042728

Feature type

allele

Associated gene

Dmel\Rho1

Associated Insertion(s)

Carried in Construct

P{GMR-Rho1}

Also Known As

GMR-RhoA, GMR-Rho1, GMR-Rho¹Rho³

Key Links

Transgenic product class

cDNA

(Hariharan et al., 1995)

wild_type

(Hariharan et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hariharan et al., 1995)

wild_type

(Hariharan et al., 1995)

Mutagen

in vitro construct

(Hariharan et al., 1995)

Progenitor genotype

Carried in construct

P{GMR-Rho1}

Cytology

Description

GMR regulatory sequences drive expression of a 779bp EcoRI fragment of Rho1 cDNA that contains the entire coding region.

(Hariharan et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Rho1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Avet-Rochex et al., 2005, Uchida et al., 2002, Morey et al., 2001, Bateman et al., 2000, Prokopenko et al., 1999, Sawamoto et al., 1999, Barrett et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Rho1^GMR.PH have a rough eye phenotype associated with the frequent fusion of ommatidial units and misoriented or missing bristles.

(Avet-Rochex et al., 2005)

Rho1^GMR.PH flies display a rough eye phenotype.

(Chen et al., 2005)

Rho1^GMR.PH flies have a rough eye phenotype.

(Tan et al., 2003)

Flies expressing Rho1^GMR.PH have a rough eye phenotype with a dramatic disruption of the ommatidial architecture. The retina is reduced in thickness and the lattices that are normally formed by the secondary and tertiary pigment cells are completely missing. Pigment cells are restricted to apical regions of the retina.

(Uchida et al., 2002)

Flies carrying two copies of the P{GMR-Rho1} insertion responsible for this allele, have an intermediate rough eye phenotype.

(Morey et al., 2001)

Rough patterned eye with misshapen, fused and flattened ommatidia.

(Bateman et al., 2000)

Expression of Rho1^GMR.PH in a wild-type background does not disrupt photoreceptor axon projections.

(Newsome et al., 2000)

Causes a rough eye phenotype.

(Prokopenko et al., 1999)

Pigment cells fail to extend to the retinal floor and the layer of pigment cell feet seen at the floor of the retina in wild-type animals is not seen in flies carrying one copy of Rho1^GMR.PH.

(Sawamoto et al., 1999)

Flies carrying Rho1^GMR.PH have rough eyes, with both the external and internal ordered structure of the eye being disrupted. The thickness of the retina is reduced.

(Barrett et al., 1997)

One copy of P{GMR-Rho1} allows normal external morphology, internal structure shows rhabdomeres are arranged in an irregular manner, but the eyes deform easily on application of mild pressure compared to wild type. Two copies, obtained by making the insertions homozygous, cause extremely rough eyes and flies carrying two copies, generated by crossing lines, have moderately rough eyes with a glassy appearance. Rough eye phenotype is due to a dramatic disruption of ommatidial architecture caused by disruption of cell differentiation.

(Hariharan et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rho1^GMR.PH has visible phenotype, enhanceable by CE2-1^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, enhanceable by CE3-1^unspecified

(Sullivan and Rubin, 2002)

NOT Enhanced by

Statement

Reference

Rho1^GMR.PH has visible phenotype, non-enhanceable by CE3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by CE3-3^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by CS3-1^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by CS3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by CS3-4^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by CanB2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by sty^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by CE2-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-enhanceable by CE2-3^unspecified

(Sullivan and Rubin, 2002)

Suppressed by

Statement

Reference

Rho1^GMR.PH has visible phenotype, suppressible by Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Rho1^GMR.PH has visible phenotype, suppressible by Parp1^GMR.PU

(Uchida et al., 2002)

Rho1^GMR.PH has visible phenotype, suppressible by dia²

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by pbl¹

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by pbl²

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by pbl³

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by pbl⁵

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by pbl^P81

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by pbl^S23

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by pbl^V58

(Prokopenko et al., 1999)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2⁰⁴²⁹¹

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^1.1

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^2.3

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^2.6

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^2.9

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^4.1

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^4.4

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^5.1

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^5.2

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^6.5

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^8.1

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^8.9

(Barrett et al., 1997)

Rho1^GMR.PH has visible phenotype, suppressible by RhoGEF2^9.6

(Barrett et al., 1997)

NOT suppressed by

Statement

Reference

Rho1^GMR.PH has visible phenotype, non-suppressible by CE2-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by CE2-3^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by CE3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by CE3-3^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by CS3-1^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by CS3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by CS3-4^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by sty^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by CanB2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has visible phenotype, non-suppressible by Sps1^k11320

(Morey et al., 2001)

Rho1^GMR.PH has visible phenotype, non-suppressible by trio[+]/trio^2H

(Bateman et al., 2000)

Enhancer of

Statement

Reference

Rho1^GMR.PH is an enhancer of visible phenotype of Hsap\RALA^G23V.GMR

(Sawamoto et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rho1^GMR.PH has interommatidial bristle phenotype, enhanceable by Mbs[+]/Mbs⁰³⁸⁰²

(Tan et al., 2003)

Rho1^GMR.PH has eye phenotype, enhanceable by Mbs[+]/Mbs⁰³⁸⁰²

(Tan et al., 2003)

Rho1^GMR.PH has eye phenotype, enhanceable by CE2-1^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, enhanceable by CE3-1^unspecified

(Sullivan and Rubin, 2002)

NOT Enhanced by

Statement

Reference

Rho1^GMR.PH has eye phenotype, non-enhanceable by CE3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by CE3-3^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by CS3-1^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by CS3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by CS3-4^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by CanB2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by sty^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by CE2-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-enhanceable by CE2-3^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has phenotype, non-enhanceable by osa^E65

(Staehling-Hampton et al., 1999)

Suppressed by

Statement

Reference

Rho1^GMR.PH has ommatidium phenotype, suppressible by Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Rho1^GMR.PH has interommatidial bristle phenotype, suppressible by Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Rho1^GMR.PH has eye phenotype, suppressible by Pax^GMR.PC

(Chen et al., 2005)

Rho1^GMR.PH has ommatidium phenotype, suppressible by Parp1^GMR.PU

(Uchida et al., 2002)

Rho1^GMR.PH has pigment cell phenotype, suppressible by Parp1^GMR.PU

(Uchida et al., 2002)

Rho1^GMR.PH has phenotype, suppressible by lilli^XS407

(Tang et al., 2001)

Rho1^GMR.PH has phenotype, suppressible by lilli^s35

(Tang et al., 2001)

Rho1^GMR.PH has phenotype, suppressible by lilli^XS575

(Tang et al., 2001)

Rho1^GMR.PH has eye phenotype, suppressible by dia²

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by pbl¹

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by pbl²

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by pbl³

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by pbl⁵

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by pbl^P81

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by pbl^S23

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by pbl^V58

(Prokopenko et al., 1999)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2⁰⁴²⁹¹

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^1.1

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^2.3

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^2.6

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^2.9

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^4.1

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^4.4

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^5.1

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^5.2

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^6.5

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^8.1

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^8.9

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by RhoGEF2^9.6

(Barrett et al., 1997)

Rho1^GMR.PH has eye phenotype, suppressible by Cdc37^e1E

(Hariharan et al., 1995)

NOT suppressed by

Statement

Reference

Rho1^GMR.PH has eye phenotype, non-suppressible by CE2-3^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by CE3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by CE3-3^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by CS3-1^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by CS3-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by CS3-4^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by CanB2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by sty^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by CE2-2^unspecified

(Sullivan and Rubin, 2002)

Rho1^GMR.PH has eye phenotype, non-suppressible by trio[+]/trio^2H

(Bateman et al., 2000)

Rho1^GMR.PH has ommatidium phenotype, non-suppressible by trio[+]/trio^2H

(Bateman et al., 2000)

Rho1^GMR.PH has eye phenotype, non-suppressible by mbc^1.63

(Nolan et al., 1998)

Rho1^GMR.PH has eye phenotype, non-suppressible by mbc^2.35

(Nolan et al., 1998)

Rho1^GMR.PH has eye phenotype, non-suppressible by mbc^3.5

(Nolan et al., 1998)

Rho1^GMR.PH has eye phenotype, non-suppressible by mbc^4.25

(Nolan et al., 1998)

Enhancer of

Statement

Reference

Rho1^GMR.PH is an enhancer of eye phenotype of Hsap\RALA^G23V.GMR

(Sawamoto et al., 1999)

Rho1^GMR.PH is an enhancer of ommatidium phenotype of Hsap\RALA^G23V.GMR

(Sawamoto et al., 1999)

NOT Enhancer of

Statement

Reference

Rho1^GMR.PH is a non-enhancer of phenotype of trio^{GEF1.GMR.Tag:MYC}

(Newsome et al., 2000)

NOT Suppressor of

Statement

Reference

Rho1^GMR.PH is a non-suppressor of phenotype of trio^{GEF1.GMR.Tag:MYC}

(Newsome et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The rough-eye phenotype of Rho1^GMR.PH flies is much reduced when Pax^GMR.PC is co-expressed.

(Chen et al., 2005)

The rough eye phenotype of Rho1^GMR.PH flies is mildly enhanced by Mbs⁰³⁸⁰²/+ (fewer bristles are formed).

(Tan et al., 2003)

The Rho1^GMR.PH phenotype is partially suppressed by Parp^GMR.PU; ommatidial lattices are seen in the rescued flies and pigment cells are seen in deeper regions of the retina. A distinct rhabdomere structure also appears.

(Uchida et al., 2002)

Does not significantly alter the photoreceptor axon projection defects caused by expression of trio^{GEF1.GMR.T:Hsap\MYC}.

(Newsome et al., 2000)

The rough eye phenotype caused by Rho1^GMR.PH is suppressed by RhoGEF2⁰⁴²⁹¹, RhoGEF2^1.1, RhoGEF2^2.3, RhoGEF2^2.6, RhoGEF2^2.9, RhoGEF2^4.1, RhoGEF2^4.4, RhoGEF2^5.1, RhoGEF2^5.2, RhoGEF2^6.5, RhoGEF2^8.1, RhoGEF2^8.9 and RhoGEF2^9.6.

(Barrett et al., 1997)

Xenogenetic Interactions

Statement

Reference

Co-expression of Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^GMR.PF restores the eye morphology to wild type in Rho1^GMR.PH flies.

(Avet-Rochex et al., 2005)

The Hsap\RALA^G23V.GMR eye phenotype is enhanced by Rho1^GMR.PH; flies carrying one copy of either Hsap\RALA^G23V.GMR or Rho1^GMR.PH have a mild rough eye phenotype, while flies carrying both Hsap\RALA^G23V.GMR and Rho1^GMR.PH have extremely rough eyes with a glossy appearance due to the fusion of facets. A thin layer of pigment cells is seen in the apical region of the retina and no recognisable ommatidia can be seen.

(Sawamoto et al., 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rho1^GMR.PH

Rho1^gl

(Sullivan and Rubin, 2002)

Name Synonyms

glass multimer reporter construct of Hariharan

(Hariharan et al., 1995)

Secondary FlyBase IDs

References (17)

Report Sections

Open Close