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General Information
Symbol
Dmel\dia2
Species
D. melanogaster
Name
FlyBase ID
FBal0039243
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Linked to:
NheI-BamHI restriction fragment
Comment:

The approximate boundaries of the dia2 deletion map to a NheI-XbaI restriction fragment; the position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

At least 5kb of DNA from the dia locus is deleted.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygotes survive embryogenesis.

Embryos derived from dia5 homozygous female germline clones which and which are also zygotically mutant for dia (dia2/dia5) have abnormal cell protrusions that extend from the amnioserosa cells during dorsal closure. Dorsal closure is defective, with cell misalignment as the epidermal sheets meet at the dorsal midline.

Early pupal lethality and absence of imaginal discs. Larval neuroblasts are enormously polyploid and have very few mitotic figures.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

dia2 is a suppressor of visible phenotype of Rho1GMR.PH

Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

dia2/dia[+] is an enhancer of spindle | maternal effect | cleavage stage phenotype of Abl4

dia2/dia[+] is an enhancer of energid phenotype of CycB+t10

Suppressor of
Statement
Reference

dia2/dia[+] is a suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

dia2/dia[+] is a suppressor | partially of mitotic domain 1 | embryonic cycle 10 phenotype of CycB+t10

dia2 is a suppressor of eye phenotype of Rho1GMR.PH

NOT Suppressor of
Statement
Reference

dia2/dia[+] is a non-suppressor of aster | embryonic cycle 5 phenotype of CycB+t10

dia2/dia[+] is a non-suppressor of aster | embryonic cycle 6 phenotype of CycB+t10

dia2/dia[+] is a non-suppressor of aster | embryonic cycle 7 phenotype of CycB+t10

Other
Additional Comments
Genetic Interactions
Statement
Reference

dia2 homozygotes in which maternal Rho1 function is reduced (derived from Rho172O heterozygous females) nearly all die as embryos, with defects in head involution, ventral cuticle or the completion of dorsal closure.

Maternal heterozygosity for dia2 enhances the dia2 homozygous phenotype, resulting in ventral holes in the cuticle.

13% of the progeny of dia2 Rho172O double heterozygous females die as embryos, largely with defects in head involution. These embryos show dramatic alterations in amnioserosal cell behaviour compared to wild type; during germband retraction, amnioserosal cells along all the germband of the mutant embryos form long persistant cell extensions extending over neighbouring epidermal cells. The protrusions are especially prominent caudally, but are also observed laterally and anteriorly. Long protrusions are occasionally seen from epidermal cells and amnioserosal cells also extend abnormal processes over one another as well as over epidermal cells. Dorsal closure in these embryos is otherwise normal, except that drop-out cells appear much earlier in dorsal closure than normal.

The progeny of dia2 RhoGEF204291 double heterozygous females have abnormal protrusions extending from amnioserosa cells over the adjacent epidermis during dorsal closure.

Heterozygosity for dia2 enhances the Abl4 phenotype.

Dominantly suppresses the rough eye phenotype of the Rho1GMR.PH and pblScer\UAS.cPa expressed under the control of Scer\GAL4GMR.PF, and dominantly enhances the eye phenotype of pblΔDH497-549.Scer\UAS expressed under the control of Scer\GAL4GMR.PF.

Xenogenetic Interactions
Statement
Reference

The abnormal cell protrusions that are seen extending from amnioserosa cells in the progeny of dia2 Rho172O double heterozygous females during dorsal closure are suppressed by expression of Ggal\MLCKct.Scer\UAS under the control of Scer\GAL4e22c.

Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

One of 17 alleles isoalted in a reversion screen of dia1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)