The approximate boundaries of the dia2 deletion map to a NheI-XbaI restriction fragment; the position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.
At least 5kb of DNA from the dia locus is deleted.
amnioserosa | maternal effect (with dia5)
larval central nervous system & neuroblast
Homozygotes survive embryogenesis.
Embryos derived from dia5 homozygous female germline clones which and which are also zygotically mutant for dia (dia2/dia5) have abnormal cell protrusions that extend from the amnioserosa cells during dorsal closure. Dorsal closure is defective, with cell misalignment as the epidermal sheets meet at the dorsal midline.
Early pupal lethality and absence of imaginal discs. Larval neuroblasts are enormously polyploid and have very few mitotic figures.
dia2/dia[+] is an enhancer of mitotic cell cycle defective | maternal effect phenotype of Abl4
dia2/dia[+] is an enhancer of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, pblΔDH497-549.UAS
dia2 is a suppressor of visible phenotype of Rho1GMR.PH
Rho172O/Rho1[+], dia2 has lethal | embryonic stage phenotype
Rho172O/Rho1[+], dia2 has amnioserosa | maternal effect phenotype, suppressible by Ggal\MLCKct.UAS/Scer\GAL4e22c
dia2/dia[+] is an enhancer of embryonic/first instar larval cuticle phenotype of Rho172O
dia2/dia[+] is an enhancer of actin cytoskeleton | maternal effect | embryonic stage phenotype of Abl4
dia2/dia[+] is an enhancer of spindle | maternal effect | cleavage stage phenotype of Abl4
dia2/dia[+] is an enhancer of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, pblΔDH497-549.UAS
dia2/dia[+] is a suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10
dia2/dia[+] is a suppressor | partially of mitotic domain 1 | embryonic cycle 10 phenotype of CycB+t10
dia2 is a suppressor of eye phenotype of Rho1GMR.PH
dia2/dia[+] is a non-suppressor of aster | embryonic cycle 5 phenotype of CycB+t10
dia2/dia[+] is a non-suppressor of aster | embryonic cycle 6 phenotype of CycB+t10
dia2/dia[+] is a non-suppressor of aster | embryonic cycle 7 phenotype of CycB+t10
Rho172O/Rho1[+], dia2 has embryonic head phenotype
Rho172O/Rho1[+], dia2 has embryonic/first instar larval cuticle phenotype
Rho172O/Rho1[+], dia2 has amnioserosa | maternal effect phenotype
RhoGEF204291/RhoGEF2[+], dia2 has amnioserosa | maternal effect phenotype
dia2 homozygotes in which maternal Rho1 function is reduced (derived from Rho172O heterozygous females) nearly all die as embryos, with defects in head involution, ventral cuticle or the completion of dorsal closure.
Maternal heterozygosity for dia2 enhances the dia2 homozygous phenotype, resulting in ventral holes in the cuticle.
13% of the progeny of dia2 Rho172O double heterozygous females die as embryos, largely with defects in head involution. These embryos show dramatic alterations in amnioserosal cell behaviour compared to wild type; during germband retraction, amnioserosal cells along all the germband of the mutant embryos form long persistant cell extensions extending over neighbouring epidermal cells. The protrusions are especially prominent caudally, but are also observed laterally and anteriorly. Long protrusions are occasionally seen from epidermal cells and amnioserosal cells also extend abnormal processes over one another as well as over epidermal cells. Dorsal closure in these embryos is otherwise normal, except that drop-out cells appear much earlier in dorsal closure than normal.
The progeny of dia2 RhoGEF204291 double heterozygous females have abnormal protrusions extending from amnioserosa cells over the adjacent epidermis during dorsal closure.
Dominantly suppresses the rough eye phenotype of the Rho1GMR.PH and pblScer\UAS.cPa expressed under the control of Scer\GAL4GMR.PF, and dominantly enhances the eye phenotype of pblΔDH497-549.Scer\UAS expressed under the control of Scer\GAL4GMR.PF.
The abnormal cell protrusions that are seen extending from amnioserosa cells in the progeny of dia2 Rho172O double heterozygous females during dorsal closure are suppressed by expression of Ggal\MLCKct.Scer\UAS under the control of Scer\GAL4e22c.
Complements: vari03953b.
One of 17 alleles isoalted in a reversion screen of dia1.