FB2020_06, released Dec 22, 2020

Allele: Dmel\RhoGEF24.4

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General Information
Symbol
Dmel\RhoGEF24.4
Species
D. melanogaster
Name
FlyBase ID
FBal0085929
Feature type
allele
Associated gene
Dmel\RhoGEF2
Associated Insertion(s)
Carried in Construct
Genomic Maps
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Nikolaidou, 2004)
Mutagen
ethyl methanesulfonate
(Barrett et al., 1997)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
2R:17,031,888..17,031,888 [+]
Nucleotide change:

C17031888T

Amino acid change:

R264term | RhoGEF2-PD; R264term | RhoGEF2-PE; R264term | RhoGEF2-PF; R264term | RhoGEF2-PG; R264term | RhoGEF2-PH

Reported amino acid change:

R264term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Nikolaidou, 2004)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      Amino acid replacement: R264term.

      (Nikolaidou, 2004)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Late embryos from RhoGEF24.4 heterozygous mothers display a wild-type cuticle pattern, as compared to controls.

      (Johnson et al., 2017)

      RhoGEF24.4/RhoGEF24.1 animals show 98% embryonic viability, 97% first larval instar viability and 0% adult viability.

      (Nikolaidou and Barrett, 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      RhoGEF24.4 is a suppressor of visible phenotype of Rho1GMR.PH

      (Barrett et al., 1997)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      RhoGEF2[+]/RhoGEF24.4 is an enhancer of embryonic/larval cuticle | ventral | maternal effect | embryonic stage phenotype of tslΔ

      (Johnson et al., 2017)
      Suppressor of
      Statement
      Reference

      RhoGEF24.4 is a suppressor of eye phenotype of Rho1GMR.PH

      (Barrett et al., 1997)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      RhoGEF24.4 heterozygosity strongly enhances the ventral cuticle defects displayed by late embryos from tslΔ homozygous mothers, leading to fully penetrant absence of ventral cuticle.

      (Johnson et al., 2017)

      Dominantly suppresses the Rho1GMR.PH rough eye phenotype.

      (Barrett et al., 1997)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Isolated as a dominant suppressor of the Rho1GMR.PH rough eye phenotype.

      (Barrett et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      4.4
      (Nikolaidou, 2004, Barrett et al., 1997)
      DRhoGEF24.4
      (Nikolaidou and Barrett, 2004)
      RhoGEF24.4
      (Johnson et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)