FlyBase Allele Report: Dmel\Tsc1[UAS.cPa]

General Information

Symbol

Dmel\Tsc1^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Potter

FlyBase ID

FBal0123962

Feature type

allele

Associated gene

Dmel\Tsc1

Associated Insertion(s)

Carried in Construct

P{UAS-Tsc1.P}

Also Known As

UAS-Tsc1

Key Links

Transgenic product class

cDNA

(Potter et al., 2001)

wild_type

(Potter et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Potter et al., 2001)

wild_type

(Potter et al., 2001)

Mutagen

in vitro construct

(Potter et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Tsc1.P}

Cytology

Description

UASt regulatory sequences drive expression of a 3.8kb Bgl2/Xho1 full length Tsc1 cDNA.

(Potter et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tsc1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Tsc1^Scer\UAS.cPa under the control of Scer\GAL4^ey.PH or Scer\GAL4^GMR.PF has no affect on cell size, cell number or organ size in the eye. Expression of Tsc1^Scer\UAS.cPa in the posterior compartment of the wing disc under the control of Scer\GAL4^en-e16E has no effect; cell size, cell number and size of the wing are normal.

(Potter et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size | somatic clone | larval stage phenotype, suppressible | partially by Cpsf6^KK108487, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size | somatic clone | larval stage phenotype, suppressible | partially by Cpsf160^HMC03912, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size | somatic clone | larval stage phenotype, suppressible | partially by CstF64^GD9942, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size | somatic clone | larval stage phenotype, suppressible | partially by Cdk8^GD7022, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size | somatic clone | larval stage phenotype, suppressible | partially by CycC^GD17120, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size | somatic clone | larval stage phenotype, suppressible | partially by Doa^KK111879, Scer\GAL4^Act.PU

(Tang et al., 2018)

Suppressor of

Statement

Reference

gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ppk.PG is a suppressor | partially of abnormal neuroanatomy | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ey.PH is a suppressor of visible phenotype of InR^UAS.cHa, Scer\GAL4^ey.PH

(Potter et al., 2001)

gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ey.PH is a suppressor of lethal | heat sensitive phenotype of InR^UAS.cHa, Scer\GAL4^ey.PH

(Potter et al., 2001)

NOT Suppressor of

Statement

Reference

Tsc1^UAS.cPa, Scer\GAL4^ey.PH is a non-suppressor of lethal | heat sensitive phenotype of InR^UAS.cHa, Scer\GAL4^ey.PH

(Potter et al., 2001)

Tsc1^UAS.cPa, Scer\GAL4^ey.PH is a non-suppressor of visible phenotype of InR^UAS.cHa, Scer\GAL4^ey.PH

(Potter et al., 2001)

Other

Statement

Reference

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size | somatic clone | larval stage phenotype

(Tang et al., 2018)

Scer\GAL4^{E(spl)m6-BFM.PF}, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell number | third instar larval stage phenotype

(Aradhya et al., 2015)

Scer\GAL4^en-e16E, Tsc1^UAS.cPa, gig^UAS.cTa has visible phenotype

(Potter et al., 2001)

Scer\GAL4^en-e16E, Tsc1^UAS.cPa, gig^UAS.cTa has decreased cell size phenotype

(Potter et al., 2001)

Scer\GAL4^en-e16E, Tsc1^UAS.cPa, gig^UAS.cTa has some die during P-stage phenotype

(Potter et al., 2001)

Scer\GAL4^GMR.PF, Tsc1^UAS.cPa, gig^UAS.cTa has visible phenotype

(Potter et al., 2001)

Scer\GAL4^ey.PH, Tsc1^UAS.cPa, gig^UAS.cTa has visible phenotype

(Potter et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has autophagosome | somatic clone | larval stage phenotype, suppressible | partially by Cpsf6^KK108487, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has embryonic/larval fat body | somatic clone | larval stage phenotype, suppressible | partially by Cpsf160^HMC03912, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has autophagosome | somatic clone | larval stage phenotype, suppressible | partially by Cpsf160^HMC03912, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has embryonic/larval fat body | somatic clone | larval stage phenotype, suppressible | partially by CstF64^GD9942, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has autophagosome | somatic clone | larval stage phenotype, suppressible | partially by CstF64^GD9942, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has embryonic/larval fat body | somatic clone | larval stage phenotype, suppressible | partially by Cdk8^GD7022, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has autophagosome | somatic clone | larval stage phenotype, suppressible | partially by Cdk8^GD7022, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has embryonic/larval fat body | somatic clone | larval stage phenotype, suppressible | partially by CycC^GD17120, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has autophagosome | somatic clone | larval stage phenotype, suppressible | partially by CycC^GD17120, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has embryonic/larval fat body | somatic clone | larval stage phenotype, suppressible | partially by Doa^KK111879, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has autophagosome | somatic clone | larval stage phenotype, suppressible | partially by Doa^KK111879, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has embryonic/larval fat body | somatic clone | larval stage phenotype, suppressible | partially by Cpsf6^KK108487, Scer\GAL4^Act.PU

(Tang et al., 2018)

Scer\GAL4^GMR.PF, Tsc1^UAS.cPa, gig^UAS.cTa has eye phenotype, suppressible | partially by Rheb^UAS.cSa, Scer\GAL4^GMR.PF

(Saucedo et al., 2003)

Suppressor of

Statement

Reference

gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ppk.PG is a suppressor | partially of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ey.PH is a suppressor of ommatidium phenotype of InR^UAS.cHa, Scer\GAL4^ey.PH

(Potter et al., 2001)

NOT Suppressor of

Statement

Reference

Tsc1^UAS.cPa, Scer\GAL4^ey.PH is a non-suppressor of ommatidium phenotype of InR^UAS.cHa, Scer\GAL4^ey.PH

(Potter et al., 2001)

Other

Statement

Reference

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has embryonic/larval fat body | somatic clone | larval stage phenotype

(Tang et al., 2018)

Scer\GAL4^Act.PU, Tsc1^UAS.cPa, gig^UAS.cTa has autophagosome | somatic clone | larval stage phenotype

(Tang et al., 2018)

Scer\GAL4^{E(spl)m6-BFM.PF}, Tsc1^UAS.cPa, gig^UAS.cTa has abdominal lateral adult muscle precursor cell | third instar larval stage phenotype

(Aradhya et al., 2015)

Scer\GAL4^{E(spl)m6-BFM.PF}, Tsc1^UAS.cPa, gig^UAS.cTa has abdominal dorsal adult muscle precursor cell | third instar larval stage phenotype

(Aradhya et al., 2015)

Scer\GAL4^{E(spl)m6-BFM.PF}, Tsc1^UAS.cPa, gig^UAS.cTa has abdominal ventral adult muscle precursor cell | third instar larval stage phenotype

(Aradhya et al., 2015)

Scer\GAL4^GMR.PF, Tsc1^UAS.cPa, gig^UAS.cTa has eye phenotype

(Saucedo et al., 2003, Potter et al., 2001)

Scer\GAL4^en-e16E, Tsc1^UAS.cPa, gig^UAS.cTa has wing blade posterior compartment phenotype

(Potter et al., 2001)

Scer\GAL4^GMR.PF, Tsc1^UAS.cPa, gig^UAS.cTa has ommatidium phenotype

(Potter et al., 2001)

Scer\GAL4^ey.PH, Tsc1^UAS.cPa, gig^UAS.cTa has eye phenotype

(Potter et al., 2001)

Scer\GAL4^ey.PH, Tsc1^UAS.cPa, gig^UAS.cTa has ommatidium phenotype

(Potter et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Under standard nutritional conditions, larval fat body clones co-expressing Tsc1^UAS.cPa and gig^UAS.cTa under the control of Scer\GAL4^Act.PU show a significant increase in autophagosomes (identified as mCherry-Atg8 puncta).

(Tang et al., 2018)

The co-expression of Tsc1^Scer\UAS.cPa and gig^Scer\UAS.cTa under the control of Scer\GAL4^{E(spl)m6-BFM.PF} leads to significantly decreased numbers of abdominal lateral, dorsal and ventral adult muscle precursor cells in mid-third instar larvae, as compared to controls.

(Aradhya et al., 2015)

Expression of Tsc1^Scer\UAS.cPa and gig^Scer\UAS.cTa suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19^GD9650 under the control of Scer\GAL4^ppk.PG at 16 hours after pupariation.

(Wong et al., 2013)

Tsc1^Scer\UAS.cPa; gig^Scer\UAS.cTa; Scer\GAL4^GMR.PF animals have small eyes. This growth suppression is partially reversed by Rheb^Scer\UAS.cSa.

(Saucedo et al., 2003)

Coexpression of gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cPa under the control of Scer\GAL4^ey.PH results in a significant decrease in overall eye size and number of ommatidia. Coexpression of gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF results in eyes that are flatter and smaller than normal but which contain normal numbers of ommatidia. These ommatidia are 30% smaller than normal. The small ommatidium size caused by coexpression of gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF is suppressed by coexpression of S6k^Scer\UAS.cWa. Coexpression of gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cPa in the posterior compartment of the wing under the control of Scer\GAL4^en-e16E results in a significant decrease in cell size (48% reduction), cell number (15% reduction) and size of the wing. The changes in cell size and number are limited to the posterior compartment of the wing. This combination results in 65% pupal lethality. Coexpression of both gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cPa (but not either gig^Scer\UAS.cTa or Tsc1^Scer\UAS.cPa alone) rescues the lethality and extra ommatidia phenotype of flies expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH.