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General Information
Symbol
Dmel\ImpL2UAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Honegger
FlyBase ID
FBal0249385
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Imp-L2, UAS-ImpL2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of ImpL2.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ImpL2Scer\UAS.cHa in adult enterocytes (under the control of Scer\GAL4Myo31DF.PU and limited to the adult stage using Scer\GAL80ts.αTub84B) leads to increased circulating and whole-body trehalose levels, increased hemolymph volume and ovary atrophy.

Overexpression of ImpL2Scer\UAS.cHa via Scer\GAL4hs.PB results in viable flies.

Overexpression of ImpL2Scer\UAS.cHa via Scer\GAL4hs.PB increases female median lifespan, oxidative stress resistance and whole-fly lipid content, while reducing fecundity.

Overexpression of ImpL2Scer\UAS.cHa via RU486-induced Scer\GAL4Act5C.Switch.PR increases both median and maximal lifespan of female flies.

Overexpression of ImpL2Scer\UAS.cHa via RU486-induced Scer\GAL4Act5C.Switch.PR increases oxidative stress resistance of female flies, while reducing fecundity.

Overexpression of ImpL2Scer\UAS.cHa with the pan-neuronal driver Scer\GAL4elav.PLu does not extend lifespan.

Overexpression of ImpL2Scer\UAS.cHa with Scer\GAL4Akh.PG does not extend lifespan.

Overexpression of ImpL2Scer\UAS.cHa via Scer\GAL4Ilp2.215-3 significantly extends both median and maximal lifespan of female flies.

Overexpression of ImpL2Scer\UAS.cHa via RU486-induced Scer\GAL4Switch1.106 significantly extends both median and maximal lifespan of female flies.

Ubiquitous overexpression of ImpL2Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI leads to lethality.

Ubiquitous overexpression of ImpL2Scer\UAS.cHa under the control of Scer\GAL4arm.PS results in flies that are decreased in size and weight.

Overexpression of ImpL2Scer\UAS.cHa under the control of Scer\GAL4arm.PS in somatic clones results in a non-autonomous reduction of cell size.

Overexpression of ImpL2Scer\UAS.cHa driven by Scer\GAL4GMR.PF results in a strong reduction of eye size. These ImpL2Scer\UAS.cHa-overexpressing flies are of normal size.

Flies overexpressing ImpL2Scer\UAS.cHa under the control of Scer\GAL4ppl.PP display a pronounced reduction in body size and their development is delayed by two days.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT suppressed by
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of InRact.Scer\UAS does not suppress the increases in circulating and whole-body trehalose levels and hemolymph volume and ovary atrophy seen when ImpL2Scer\UAS.cHa is expressed in adult enterocytes (under the control of Scer\GAL4Myo31DF.PU and limited to the adult stage using Scer\GAL80ts.αTub84B).

Ubiquitous expression of ImpL2Scer\UAS.cHa (under the control of Scer\GAL4Act5C.PP) results in the overgrowth of Pten117 clones, irrespective of whether they neighbor Tor2L19 mutant or wild-type cells. The overgrowth of Pten117 clones causes a further reduction in body size, as evidenced by decreased shoulder width.

ImpL2Scer\UAS.cHa partially suppresses the eye overgrowth resulting from the overexpression of InRScer\UAS.cHa driven by Scer\GAL4GMR.PF.

Df(3L)AC1 dominantly enhances the small eye phenotype caused by Scer\GAL4GMR.PF-driven overexpression of ImpL2Scer\UAS.cHa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ImpL2Scer\UAS.cHa
ImpL2UAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Honegger
Secondary FlyBase IDs
    References (6)