FlyBase Allele Report: Scer\GAL4[Ilp2.215-3]

General Information

Symbol

Scer\GAL4^Ilp2.215-3

Species

S. cerevisiae

Name

FlyBase ID

FBal0141513

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Ilp2-GAL4.215-3}

Also Known As

dilp2-GAL4, dilp2GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Ikeya et al., 2002)

Progenitor genotype

Ecol\lacZ^{Ilp2.pXD215-3}

(Ikeya et al., 2002)

Carried in construct

P{Ilp2-GAL4.215-3}

Cytology

Description

The promoter fragment from Ecol\lacZ^{Ilp2.pXD215-3} (-983nt to -146nt from the Ilp2 upstream region) regulates expression of a GAL4 driver.

(Ikeya et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

Ilp2

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

neurosecretory neuron | subset of adult brain | dorsal | restricted

(Cognigni et al., 2011)

neuron | subset of adult gnathal ganglion | restricted

(Cognigni et al., 2011)

adult pars intercerebralis

(Hong et al., 2008)

adult insulin secreting cell of pars intercerebralis

(Hadjieconomou et al., 2020, Söderberg et al., 2012)

Additional Information

Statement

Reference

ScerGAL4^Ilp2.215-3 is expressed in a small group of 10-14 neurosecretory cells located in the dorsal part of the adult brain. These cells send neurites to arborize in the subesophageal ganglion.

(Cognigni et al., 2011)

Marker for

Reflects expression of

Ilp2

(Hadjieconomou et al., 2020, Cognigni et al., 2011, Hong et al., 2008)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior, with rpr^UAS.C

(Broughton et al., 2005)

abnormal chemosensory behavior | adult stage, with Dsk^GD6123

(Söderberg et al., 2012)

abnormal chemosensory behavior | adult stage, with Ork1^{Δ-C.UAS.EGFP}

(Söderberg et al., 2012)

abnormal chemosensory behavior | larval stage, with Dsk^GD6123

(Söderberg et al., 2012)

abnormal cold stress response, with 5-HT1A^KK108407

(Luo et al., 2012)

abnormal cold stress response, with 5-HT1A^RNAi.UAS

(Luo et al., 2012)

abnormal developmental rate, with rpr^UAS.C

(Ikeya et al., 2002)

abnormal feeding behavior | adult stage, with Dsk^GD6123

(Söderberg et al., 2012)

abnormal feeding behavior | adult stage, with Ork1^{Δ-C.UAS.EGFP}

(Söderberg et al., 2012)

abnormal feeding behavior | larval stage, with Dsk^GD6123

(Söderberg et al., 2012)

abnormal heat stress response, with 5-HT1A^KK108407

(Luo et al., 2012)

abnormal heat stress response, with rpr^UAS.C

(Broughton et al., 2005)

abnormal locomotor behavior | larval stage, with Dsk^GD6123

(Söderberg et al., 2012)

abnormal mating, with rpr^UAS.C

(Wigby et al., 2011)

abnormal oxidative stress response, with Cln3^UAS.cTa

(Tuxworth et al., 2011)

abnormal starvation stress response, with 5-HT1A^KK108407

(Luo et al., 2012)

abnormal starvation stress response, with 5-HT1A^RNAi.UAS

(Luo et al., 2012)

abnormal starvation stress response | adult stage, with Dsk^GD6123

(Söderberg et al., 2012)

abnormal starvation stress response | adult stage, with sug^UAS.cZa

(Varghese et al., 2010)

decreased body size, with rpr^UAS.C

(Ikeya et al., 2002)

decreased fecundity | female, with rpr^UAS.C

(Broughton et al., 2005)

lethal, with Hsap\KCNJ2^UAS.EGFP

(Hong et al., 2008)

long lived, with Cln3^UAS.cTa

(Tuxworth et al., 2011)

long lived, with rpr^UAS.C

(Broughton et al., 2005)

long lived | dominant

(Tuxworth et al., 2011)

long lived | female limited, with ImpL2^UAS.cHa

(Alic et al., 2011)

short lived, with 5-HT1A^KK108407

(Luo et al., 2012)

short lived, with 5-HT1A^RNAi.UAS

(Luo et al., 2012)

short lived, with Hsap\UCP3^UAS.cHa

(Humphrey et al., 2009)

viable, with Hsap\UCP3^UAS.cHa

(Humphrey et al., 2009)

Phenotype Manifest In

insulin secreting cell of pars intercerebralis, with rpr^UAS.C

(Broughton et al., 2005)

neurosecretory neuron, with rpr^UAS.C

(Wigby et al., 2011)

oocyte, with rpr^UAS.C

(Ikeya et al., 2002)

wing, with rpr^UAS.C

(Ikeya et al., 2002)

Detailed Description

Statement

Reference

Inhibition (using Ork1^{Δ-C.UAS.EGFP}) of Scer\GAL4^Ilp2.215-3 cells in adult males significantly increases feeding on undesirable food in a choice assay, compared to controls.

(Söderberg et al., 2012)

Flies heterozygous for Scer\GAL4^Ilp2.215-3 show extended lifespan.

(Tuxworth et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

sug^GD11090/Scer\GAL4^Ilp2.215-3 is a suppressor | partially of abnormal starvation stress response | adult stage phenotype of mir-14^Δ1

(Varghese et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

mir-14^{UAS.DsRed(Unk)}/Scer\GAL4^Ilp2.215-3 rescues mir-14^Δ1

(Varghese et al., 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^IRP.215-3

Scer\GAL4^Ilp2.215-3

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections