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General Information
Symbol
Dmel\stanUAS.cUa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Usui
FlyBase ID
FBal0101420
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Fmi, UAS-stan, fmiScer\UAS.cUa, UAS.stan
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Contains a 12.2kb fragment containing the stan ORF under the control of UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

No obvious R7 photoreceptor targeting phenotypes are seen when stanScer\UAS.cUa is expressed under the control of Scer\GAL4GMR.PF.

Expression of stanScer\UAS.cUa under the control of Scer\GAL4hs.2sev results in planar cell polarity defects in the eye.

Temperature- and dose-sensitive overexpression of stanScer\UAS.cUa with Scer\GAL4HLHmδ results in many ommatidial axon cartridges containing abnormal numbers of R cell axons. Observing single cells via dye-filling, 8/9 injected ommatidia show mistargeting and 6/8 cases show normal R4 targeting, whilst neighbouring R cells follow inappropriate trajectories. In 2/8 cases the majority of axons choose inappropriate targets and a presumptive R4 axon could not be discerned.

Expression of stanScer\UAS.cUa under the control of Scer\GAL4bi-md653 results in bands of tissue polarity changes in the wing hairs.

stanScer\UAS.cUa Scer\GAL4ptc-559.1 adults have a zone of reversed polarity at the back and behind the anterior compartment of each abdominal segment.

Expression of stanScer\UAS.cUa under the control of Scer\GAL4OK107 results in loss of the dorsal branches of mushroom body axons in the adult. The β, β' and γ lobes appear to be present. Wandering third instar larvae expressing stanScer\UAS.cUa under the control of Scer\GAL4OK107 have no detectable mushroom body phenotype. At 18 hours after puparium formation, it appears that at least 25% and perhaps all α'/β' axons have extended normally, but over the next 12-24 hours, the dorsal lobes become progressively shorter until they are not detectable at 48 hours after puparium formation.

stanScer\UAS.cUa; Scer\GAL4sev.PM181 flies display typical planar cell polarity phenotypes: defects in ommatidial chirality (random chirality and symmetrical clusters (predominantly R3/R3)) and rotation. The percentage of symmetrical (R3/R3) ommatidia is greater when the flies are raised at 25oC than at 18oC. Marker experiments show that photoreceptor cell R4 is frequently transformed into an extra R3 in these flies.

When expression is driven by Scer\GAL4bi-md653 wing hairs point away from the midline in the C region of the wing.

In embryos overexpressing stanScer\UAS.cUa dorsal dendrites fail to stop at the proper length and overextend toward the dorsal midline. The expression of stanScer\UAS.cUa in epithelial cells when driven by Scer\GAL4Kr.PM no dendritic overextension phenotype is seen.

stanScer\UAS.cUa driven by Scer\GAL4ap-md52 disrupts the planar polarity of bristles in the notum, affecting both microchaetae and macrochaetae. Macrochaetae can be seen to be pointing upwards, as opposed to posteriorly as in wild-type. During SOP pI division in flies with stanScer\UAS.cUa driven by Scer\GAL4ap-md52 the mitotic spindle is misoriented within the epithelial plane.

When transfected into a weakly aggregating S2 cell line, stanScer\UAS.cUa driven by Scer\GAL4Act5C.PHb induces cell aggregation. stanScer\UAS.cUa driven by Scer\GAL41407 in a wild-type background does not disrupt cell polarity. stanE45/stanE59 mutants partially rescued by stanScer\UAS.cUa driven by Scer\GAL41407 have planar polarity defects in ommatidia, sensory bristles and wing hairs. In region D (2nd posterior cell) of the wing, hairs are deflected from the proximo-distal axis and oriented towards the posterior wing margin. When expressed in the wing under the control of Scer\GAL4ptc-559.1, wing hairs are orientated towards the highest point of the stan gradient.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

R7 photoreceptor axons that over-express gogoScer\UAS.T1 and stanScer\UAS.cUa (under the control of Scer\GAL4GMR.PF) are redirected to the M3 layer.

Co-expression of stanScer\UAS.cUa and gogoScer\UAS.cMa under the control of Scer\GAL4GMR.PF induces mistargeting of R7 photoreceptor cells to the R8 recipient layer (M3).

Co-expression of stanScer\UAS.cUa and gogoFFD.Scer\UAS under the control of Scer\GAL4GMR.PF induces mistargeting of R7 photoreceptor cells to the R8 recipient layer (M3).

Co-expression of stanScer\UAS.cUa and gogoFFD.Scer\UAS under the control of Scer\GAL4GMR.PF does not induce mistargeting of R7 photoreceptor cells to the R8 recipient layer (M3).

Co-expression of gogoFL.Scer\UAS.T:Hsap\MYC and stanScer\UAS.cUa under the control of Scer\GAL4GMR.PU results in R7 photoreceptor cell axons mistargeting to the M3 layer of the medulla.

Co-expression of gogoΔC.Scer\UAS.T:Hsap\MYC and stanScer\UAS.cUa under the control of Scer\GAL4GMR.PU results in a subtle premature axon stopping phenotype in the R7 photoreceptor cells (7.2%).

The planar cell polarity defects in the eye which are caused by expression of stanScer\UAS.cUa under the control of Scer\GAL4hs.2sev are not suppressed by VhaM8.9EY03616/+.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are co-expressing stanScer\UAS.cUa and fzScer\UAS.cUa under the control of Scer\GAL4αTub84B.PL in a stan- background (the cells within the clone are stanE59/stanE59 while the cells surrounding the clone are stan3/stanE59) do not change the cell polarity of the stan3/stanE59 cells surrounding the clone.

The reversal of cell polarity phenotype that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing stanScer\UAS.cUa under the control of Scer\GAL4αTub84B.PL shows a longer range if the clones are induced in a dsunspecified background compared to a wild-type background.

The tissue polarity phenotype seen in the wings of flies expressing stanScer\UAS.cUa under the control of Scer\GAL4bi-md653 is blocked if the flies are simultaneously mutant for frtzunspecified, with the double mutant phenotype resembling that of frtzunspecified single mutants.

Cells within stanScer\UAS.cUa Scer\GAL4αTub84B.PL pwnunspecified/pwnunspecified clones (Scer\GAL80αTub84B.PL MARCM method) in the adult abdomen show some dishevelment. The clones induce extensive reversal behind the clone, both in the anterior and posterior compartments, and also within the clone, at the back. These clones do not cause planar repolarisation in a fz21/fz21 background.

The zone of reversed polarity at the back and behind the anterior compartment of each abdominal segment of stanScer\UAS.cUa; Scer\GAL4ptc-559.1 adults is suppressed by fz37/fz21.

The proportion of symmetrical (R3/R3) ommatidia in the eyes of stanScer\UAS.cUa; Scer\GAL4sev.PM181 flies is significantly enhanced by heterozygosity for DlRevF10 or dgo380, but is unaffected by heterozygosity for fz25, fz30, VangX, or Rho172O. The planar cell polarity phenotype displayed by the eyes of Nact.sev; stanScer\UAS.cUa ; Scer\GAL4sev.PM181 flies is distinct from that of stanScer\UAS.cUa ; Scer\GAL4sev.PM181 flies, but indistinguishable from that of Nact.sev flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of stanScer\UAS.cUa under the control of Scer\GAL4GMR.PU rescues the axon targeting defects of homozygous single cell stanE59 R8 photoreceptor clones.

The number of muscles receiving ectopic type I synapses, or innervation by type II motorneurons, is rescued by neuronal expression of stanScer\UAS.cUa in the background of stanE59/stan72.

Loss of synapses on muscles 6, 7 and 13 of stanE59/stan72 animals are completely rescued by neuronal expression of stanScer\UAS.cUa, though only partially so for muscle 12.

The average area of the segmental nerve of stanE59/Df(2R)stan2 animals, in which stanScer\UAS.cUa is driven by Scer\GAL4elav-C155, is significantly larger than in wildtype controls or in the stanE59/Df(2R)stan2 mutants. A small number of axons (2-3 per segmental nerve) axons have large vacuoles.

Lethality of stanE59/Df(2R)stan2 animals is rescued by neuronal expression of stanScer\UAS.cUa with Scer\GAL4elav-C155.

stanScer\UAS.cUa rescues the dendrite overextension phenotype seen in stanMB or stanE59 mushroom body neuroblast clones, when co-expressed in the clones using Scer\GAL4OK107.

Expression of stanScer\UAS.cUa driven by Scer\GAL41407 or Scer\GAL41407 partially rescues the multidendritic neuron overextension phenotype seen in these embryos.

The lethality and axonal defects seen in stanE45/stanE59 can be rescued by stanScer\UAS.cUa driven by Scer\GAL41407. This combination does not affect the planar polarity phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid and transfected into S2 cells in order to study the effects of stan on cell aggregation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
stanScer\UAS.cUa
stanUAS.cUa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Usui
Secondary FlyBase IDs
    References (28)