In a wild-type background, Nact.sev causes R1/R6 cells to differentiate as R7 cells.
Flies carrying Nact.sev have a rough eye phenotype and extra R7 photoreceptor cells in the ommatidia.
Nact.sev expression causes development of supernumerary R7 photoreceptor cells in expense of R1/R6 photoreceptor fate.
The eyes if Nact.sev flies have many symmetrical ommatidia. Some of these have a pair of R4s in place of an R3/R4 pair, while others have an R7 pair in place of an R1/R6 pair.
Most ommatidia are symmetrical in flies expressing Nact.sev, due to the presence of two R4 cells. Photoreceptor cells R1 and R6 also appear to be missing.
Expression of Nact.sev produces adult eyes containing ommatidia with 4-5 outer cells and up to three R7-like cells. Some ommatidia have only one or two R7 photoreceptors.
Flies expressing Nact.sev develop symmetrical ommatidia (more than 69.5% of ommatidia have this phenotype). The ommatidia have only 4 outer photoreceptor cells (R4/R4/R2/R5 based on molecular markers).
Flies expressing Nact.sev develop many symmetrical ommatidial clusters showing the R4/R4 phenotype. This phenotype is not affected by dsh1. Flies expressing Nact.sev also show loss and transformation of outer photoreceptors R1 to R6. In ommatidia that are mosaic in the R3/R4 photoreceptors, the Nact.sev cell always adopts the R4 fate. This can lead to polarity reversals.
Rough eye phenotype is observed. Expression in eye discs causes ommatidial clusters to contain 5 instead of 4 cone cells, fate of the R7 cells changes to a cone cell. Expression in R3 and R4 precursors results in transformation of these cells into two R7 cells. Phenotype is enhanced by expression of NANK.sev.
Rough eye phenotype.
Moderate roughening of the eye surface: many ommatidia display a 'partial lens' morphology.
Nact.sev has abnormal planar polarity phenotype, non-enhanceable by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has abnormal neuroanatomy | dominant | adult stage phenotype, suppressible by baz815-8
Nact.sev has visible phenotype, suppressible by Su(H)IB115
Nact.sev has visible phenotype, non-suppressible by Socs36EUASp.cCa/Scer\GAL4hs.2sev
Nact.sev has abnormal planar polarity phenotype, non-suppressible by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev is a suppressor of abnormal cell death phenotype of Psn+14.UAS, Scer\GAL4GMR.PF
AntpUAS.cBa, Nact.sev, Scer\GAL4ey.PH has wing | ectopic phenotype, enhanceable by Taf3UAS.cPa, Scer\GAL4ey.PH
AntpUAS.cBa, Nact.sev, Scer\GAL4ey.PH has eye phenotype, enhanceable by Taf3UAS.cPa, Scer\GAL4ey.PH
Nact.sev has wing | ectopic phenotype, enhanceable by AntpAAAA.UAS/Scer\GAL4ey.PH
Nact.sev has eye phenotype, enhanceable by AntpAAAA.UAS/Scer\GAL4ey.PH
Nact.sev has photoreceptor cell R3 phenotype, enhanceable by dx1
Nact.sev has photoreceptor cell R4 phenotype, enhanceable by dx1
Nact.sev has photoreceptor cell R7 phenotype, enhanceable by dx1
Nact.sev has photoreceptor cell R3 phenotype, enhanceable by Delta5F
Nact.sev has photoreceptor cell R4 phenotype, enhanceable by Delta5F
Nact.sev has photoreceptor cell R7 phenotype, enhanceable by Delta5F
Nact.sev has photoreceptor cell R3 phenotype, enhanceable by Su(H)T4
Nact.sev has photoreceptor cell R4 phenotype, enhanceable by Su(H)T4
Nact.sev has photoreceptor cell R7 phenotype, enhanceable by Su(H)T4
Nact.sev has phenotype, enhanceable by DeltaeAN13
Nact.sev has phenotype, enhanceable by DeltaeAV12
Nact.sev has phenotype, enhanceable by DeltaeAZ18
Nact.sev has phenotype, enhanceable by Su(H)eBC11
Nact.sev has phenotype, enhanceable by anon-XIIIeAH7
Nact.sev has phenotype, enhanceable by anon-XIIIeAV9
Nact.sev has phenotype, enhanceable by anon-XXeAK12
Nact.sev has phenotype, enhanceable by anon-XXeAV4
Nact.sev has ommatidium phenotype, non-enhanceable by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R1 phenotype, non-enhanceable by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R3 phenotype, non-enhanceable by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R4 | ectopic phenotype, non-enhanceable by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R6 phenotype, non-enhanceable by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R7 | ectopic phenotype, non-enhanceable by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has phenotype, non-enhanceable by lilliXS407
Nact.sev has phenotype, non-enhanceable by lilliXS575
Nact.sev has phenotype, non-enhanceable by rinunspecified
Nact.sev has photoreceptor cell R7 | increased number | adult stage phenotype, suppressible by baz815-8
Nact.sev has photoreceptor cell R7 | increased number phenotype, suppressible by emcAP6
AntpAAAA.UAS, Nact.sev, Scer\GAL4ey.PH has antenna phenotype, suppressible by exdUAS.cGa, Scer\GAL4ey.PH
AntpAAAA.UAS, Nact.sev, Scer\GAL4ey.PH has leg | ectopic phenotype, suppressible by exdUAS.cGa, Scer\GAL4ey.PH
AntpAAAA.UAS, Nact.sev, Scer\GAL4ey.PH has wing | ectopic phenotype, suppressible by exdUAS.cGa, Scer\GAL4ey.PH
Nact.sev has photoreceptor cell R3 phenotype, suppressible by Su(H)IB115
Nact.sev has photoreceptor cell R4 phenotype, suppressible by Su(H)IB115
Nact.sev has photoreceptor cell R7 phenotype, suppressible by Su(H)IB115
Nact.sev has phenotype, suppressible by anon-XIVsC12
Nact.sev has phenotype, suppressible by anon-XIVsU2
Nact.sev has phenotype, suppressible by sAM16sAM16
Nact.sev has photoreceptor cell R7 | increased number phenotype, non-suppressible by Socs36EUASp.cCa/Scer\GAL4hs.2sev
Nact.sev has eye phenotype, non-suppressible by Socs36EUASp.cCa/Scer\GAL4hs.2sev
Nact.sev has ommatidium phenotype, non-suppressible by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R1 phenotype, non-suppressible by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R3 phenotype, non-suppressible by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R4 | ectopic phenotype, non-suppressible by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R6 phenotype, non-suppressible by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has photoreceptor cell R7 | ectopic phenotype, non-suppressible by Scer\GAL4sev.PM181/stanUAS.cUa
Nact.sev has phenotype, non-suppressible by lilliXS407
Nact.sev has phenotype, non-suppressible by lilliXS575
Nact.sev has phenotype, non-suppressible by rinunspecified
Nact.sev, svp1 has photoreceptor cell R7 | ectopic | somatic clone phenotype
Nact.sev, Scer\GAL4ey.PH, UbxUAS.cPa has antenna phenotype
Nact.sev, Scer\GAL4ey.PH, UbxUAS.cPa has leg | ectopic phenotype
In a sev14; Nact.sev double mutant background, R1/R6 cells differentiate as cone cells, and, in the adult eyes only large rhabdomere cells are evident in the outer regions (R7 level) of the eye. Thus, removal of sev from Nact.sev ommatidia presents the specification of the R1/R6 cells as photoreceptors.
The rough eye and extra R7 photoreceptor cell phenotypes caused by Nact.sev are not suppressed by expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev.
Expression of AntpScer\UAS.cBa and Nact.sev under the control of Scer\GAL4ey.PH induces the ectopic outgrowth of T2-specific appendages (wings and legs) on the adult head the transformation of the anntenna into leg is not complete, owing to the fact that Scer\GAL4ey.PH is not expressed in the entire antennal disc.
Approximately 20% of Scer\GAL4ey.PH; Nact.sev; AntpAAAA.Scer\UAS flies exhibit antenna-to-leg transformation, while none of these flies show eye-to-wing transformation. Legs induced in this fashion exhibit second leg identity, growing tibial apical bristles.
Misexpression of Nact.sev with UbxScer\UAS.cPa under the control of Scer\GAL4ey.PH does not induced any ectopic wing outgrowth in the eye, but exhibits antenna-to-leg transformation.
Misexpression of Nact.sev with AntpK50.Scer\UAS under the control of Scer\GAL4ey.PH results in an arista-to-tarsus transformation phenotype. These mutants exhibit some antenna-to-leg transformation but fail to induce ectopic wing tissue in the eye.
Co-expression of exdScer\UAS.cGa in combination with AntpScer\UAS.cBa or AntpAAAA.Scer\UAS in the presence of Nact.sev all under the control of Scer\GAL4ey.PH results in approximately 2% of flies exhibiting wing structures in the eye region, compared to around 40% when exdScer\UAS.cGa is absent. Only 3% of these flies show antenna-to-leg transformation compared with 28% without exdScer\UAS.cGa. The presence of exdScer\UAS.cGa also inhibits antenna-to-leg transformation induced by AntpAAAA.Scer\UAS from 19% to 4.5% (in a Nact.sev and Scer\GAL4ey.PH background).
The planar cell polarity phenotype displayed by the eyes of Nact.sev; stanScer\UAS.cUa ; Scer\GAL4sev.PM181 flies is distinct from that of stanScer\UAS.cUa ; Scer\GAL4sev.PM181 flies, but indistinguishable from that of Nact.sev flies.
The increased level of apoptosis in eye discs of animals carrying two copies of Psn+14.Scer\UAS expressed under the control of Scer\GAL4GMR.PF is strongly reduced by the expression of Nact.sev.
Second site dominant suppressors reduce the external roughness of the eye, in addition to causing the overall eye shape to become both rounder and larger, and less refractile. Second site dominant enhancers increase the roughness of the eye, eye shape is smaller and flatter and eyes appear more glossy or shiny.