FlyBase Allele Report: Dmel\baz[815-8]

General Information

Symbol

Dmel\baz^815-8

Species

D. melanogaster

Name

FlyBase ID

FBal0051779

Feature type

allele

Associated gene

Dmel\baz

Associated Insertion(s)

Carried in Construct

Also Known As

baz^815.8

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(McKim et al., 1996)

Progenitor genotype

Cytology

Description

Amino acid replacement: E253term.

(Krahn et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:17,189,628..17,189,628 [+]

Nucleotide change:

G17189628T

Amino acid change:

E253term | baz-PA; E274term | baz-PB; E253term | baz-PC; E274term | baz-PD

Reported amino acid change:

E253term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Krahn et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | somatic clone

(Claret et al., 2014)

abnormal neurophysiology | larval stage, with Scer\GAL4^C57, baz^RNAi.cRa.UAS

(Ramachandran et al., 2009)

lethal - all die during embryonic stage | recessive

some die during embryonic stage | recessive

Phenotype Manifest In

adherens junction | pupal stage | somatic clone

(Zaessinger et al., 2015)

egg chamber | germline clone

(Huynh et al., 2001)

embryonic epidermis & spindle

(Kuchinke et al., 1998)

embryonic ganglion mother cell

(Kuchinke et al., 1998)

embryonic leading edge cell

(Pickering et al., 2013)

eye disc | pupal stage

(Zaessinger et al., 2015)

follicle cell | somatic clone

(Claret et al., 2014)

interommatidial cell | pupal stage | somatic clone | increased number

(Zaessinger et al., 2015)

oocyte & centrosome | germ-line clone

(Huynh et al., 2001)

oocyte & microtubule | germ-line clone

(Huynh et al., 2001)

presumptive embryonic/larval central nervous system

(Kuchinke et al., 1998)

section of wing margin | somatic clone

(Wu et al., 2024)

Detailed Description

Statement

Reference

Somatic clones of baz^815-8 homozygous mutant cells in the pupal eye disc show extra inter-ommatidial cells and increased frequency of disruptions in the structure of adherens junctions.

(Zaessinger et al., 2015)

Homozygous clones in the follicle cells results in multilayering of the follicle cell epithelium and gaps are also sometimes seen. Single mutant cells adjacent to wild-type cells undergo morphological changes similar to a epithelial-mesenchymal transition.

(Claret et al., 2014)

The number of filopodia at the leading edge is significantly reduced in mutant embryos.

(Pickering et al., 2013)

Larvae expressing baz^{dsRNA.cRa.Scer\UAS} under the control of Scer\GAL4^C57 in the muscles in a baz^815-8/+ background show an increase in evoked excitatory junctional potential (EJP) amplitude compared to controls, and the amplitude and frequency of miniature EJPs is increased in these animals compared to wild type.

(Ramachandran et al., 2009)

the majority of mutant egg chambers (baz^815-8 homozygous germline clones) stop growing at stage 5 and adopt an oval shape. Furthermore, none of the cells in the cyst accumulate higher levels of cortical actin that are normally found around the oocyte, though two cells with four ring canals lie at the posterior of the mutant egg chamber, as in wild-type. In females dissected 2 days post eclosion, 14% of cysts contain a normal oocyte, whereas 86% arrest at stage 5 with the 16 nurse cell phenotype. The frequency of normal egg chambers falls to about 3% if the females are dissected after 6 days. In wild-type cysts, centrosomes migrate along the fusome to cluster at the anterior of the oocyte in region 2b, and then translocate to the posterior of the oocyte in region 3. In mutant cysts, the centrosomes accumulate at the anterior of the oocyte, but never move to the posterior cortex. Microtubules also remain focused on the anterior of the oocyte and fail to rearrange.

(Huynh et al., 2001)

The mitotic spindle of trunk epidermal cells occasionally has an aberrant orientation in embryos. Defects in localisation of ganglion mother cells are seen. The longitudinal axon pathway that is formed by fasciculation of the MP1, pCC, dMP2 and vMP2 neurons does not form.

(Kuchinke et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

baz^815-8/baz[+] is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

Suppressor of

Statement

Reference

baz^815-8 is a suppressor of abnormal neuroanatomy | dominant | adult stage phenotype of N^act.sev

(Wu et al., 2024)

Other

Statement

Reference

Cdk1^as2, baz^{ΔLB.Ubi.EGFP}, baz^815-8 has abnormal cell polarity | larval stage | chemical conditional phenotype

(Loyer et al., 2024)

Cdk1^as2, baz^{ΔOD.Ubi.EGFP}, baz^815-8 has abnormal cell polarity | larval stage | chemical conditional phenotype

(Loyer et al., 2024)

aPKC^k06403/Df(2R)BSC330, baz^815-8 has viable phenotype

(Besson et al., 2015)

aPKC^k06403/Df(2R)BSC330, baz^815-8 has fertile phenotype

(Besson et al., 2015)

Pten^dj189/Pten[+], baz^815-8 has abnormal neuroanatomy | dominant | larval stage phenotype

(Ramachandran et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

baz^815-8 is an enhancer of wing margin | distal phenotype of N^55e11

(Wu et al., 2024)

baz^815-8/baz[+] is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

NOT Enhancer of

Statement

Reference

baz^815-8/baz[+] is a non-enhancer of wing hair phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

Suppressor of

Statement

Reference

baz^815-8 is a suppressor of photoreceptor cell R7 | increased number | adult stage phenotype of N^act.sev

(Wu et al., 2024)

NOT Suppressor of

Statement

Reference

baz^815-8/baz[+] is a non-suppressor of wing hair phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

Other

Statement

Reference

Cdk1^as2, baz^{ΔOD.Ubi.EGFP}, baz^815-8 has apical part of cell | larval stage | chemical conditional phenotype

(Loyer et al., 2024)

Cdk1^as2, baz^{ΔLB.Ubi.EGFP}, baz^815-8 has larval neuroblast | chemical conditional phenotype

(Loyer et al., 2024)

Cdk1^as2, baz^{ΔLB.Ubi.EGFP}, baz^815-8 has apical part of cell | larval stage | chemical conditional phenotype

(Loyer et al., 2024)

Cdk1^as2, baz^{ΔOD.Ubi.EGFP}, baz^815-8 has larval neuroblast | chemical conditional phenotype

(Loyer et al., 2024)

N^55e11, baz^815-8 has posterior wing margin | proximal phenotype

(Wu et al., 2024)

Pten^dj189/Pten[+], baz^815-8 has NMJ bouton phenotype

(Ramachandran et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

baz^815-8/+ enhances the planar cell polarity (PCP) defects in the eye induced by the overexpression of dgo^Scer\UAS.cFa under the control of Scer\GAL4^hs.2sev.

(Weber et al., 2012)

Pten^dj189/+ baz^815-8/+ double heterozygous larvae have a significant reduction in the number of synaptic boutons at the neuromuscular junction compared to wild type.

(Ramachandran et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

baz^815-8 is rescued by Scer\GAL4^da.G32/baz^UAS.GFP

(Krahn et al., 2010)

baz^815-8 is rescued by Scer\GAL4^da.G32/baz^{ΔPDZ3.UAS.GFP}

(Krahn et al., 2010)

baz^815-8 is rescued by baz^{Δ1097-1464.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is rescued by Scer\GAL4^da.G32/baz^{Δ1222-1464.UAS.GFP}

(Krahn et al., 2010)

baz^815-8 is rescued by baz^{Δ1325-1464.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is rescued by baz^{Δ1461-1464.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is rescued by baz^{Δ1173-1193.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is rescued by baz^{Δ1107-1464.UAS.GFP.Tag:PH(hPLCD1)}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is rescued by baz^{S151A.S1085A.UASp.GFP}/Scer\GAL4^e22c

(Benton and St. Johnston, 2003)

baz^815-8 is rescued by baz^UASp.GFP/Scer\GAL4^e22c

(Benton and St. Johnston, 2003)

baz^815-8 is rescued by baz^UASp.GFP/Scer\GAL4^arm.PS

(Benton and St. Johnston, 2003)

baz^815-8 is rescued by baz^UASp.GFP/Scer\GAL4^e22c

(Benton and St. Johnston, 2003)

Partially rescued by

baz^815-8 is partially rescued by baz^ΔN.UASp.GFP/Scer\GAL4^arm.PS

(Benton and St. Johnston, 2003)

baz^815-8 is partially rescued by baz^ΔN.UASp.GFP/Scer\GAL4^e22c

(Benton and St. Johnston, 2003)

Not rescued by

baz^815-8 is not rescued by baz^{Δ1-317.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is not rescued by baz^{ΔPDZ1.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is not rescued by baz^{ΔPDZ2.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is not rescued by Scer\GAL4^da.G32/baz^{ΔPDZ1-3.UAS.GFP}

(Krahn et al., 2010)

baz^815-8 is not rescued by baz^{Δ969-1464.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is not rescued by baz^{Δ1001-1464.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is not rescued by baz^{Δ1073-1093.UAS.GFP}/Scer\GAL4^da.G32

(Krahn et al., 2010)

baz^815-8 is not rescued by Scer\GAL4^da.G32/baz^{Δ1-904.UAS.GFP}

(Krahn et al., 2010)

baz^815-8 is not rescued by Scer\GAL4^arm.PS/baz^N.UASp.GFP

(Benton and St. Johnston, 2003)

baz^815-8 is not rescued by baz^N.UASp.GFP/Scer\GAL4^e22c

(Benton and St. Johnston, 2003)

Comments

Expression of baz^{Scer\UAS.T:Avic\GFP.cKa} driven by Scer\GAL4^da.G32 rescues the embryonic lethality of baz^815-8.

Expression of baz^{ΔPDZ3.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 rescues the lethality of baz^815-8.

Expression of baz^{Δ1097-1464.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 rescues the lethality of baz^815-8.

Expression of baz^{Δ1222-1464.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 rescues the lethality of baz^815-8.

Expression of baz^{Δ1325-1464.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 rescues the lethality of baz^815-8.

Expression of baz^{Δ1461-1464.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 rescues the lethality of baz^815-8.

Expression of baz^{Δ1173-1193.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 rescues the lethality of baz^815-8.

Expression of baz^{Δ1107-1464.Scer\UAS.T:Avic\GFP.T:Hsap\PLCD1} driven by Scer\GAL4^da.G32 rescues the lethality of baz^815-8.

Expression of baz^{Δ1-317.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{ΔPDZ1.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{ΔPDZ2.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{ΔPDZ1-3.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{Δ969-1464.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{Δ1001-1464.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{Δ968-996.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{Δ1073-1093.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

Expression of baz^{Δ1-904.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^da.G32 fails to rescue the lethality of baz^815-8.

(Krahn et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

41783

w^* baz^815-8 P{FRT(w^hs)}9-2/FM7a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

baz^815-8

(Loyer et al., 2024, Wu et al., 2024, Chen et al., 2018, Kullmann and Krahn, 2018, Sen et al., 2015, Zaessinger et al., 2015, Pickering et al., 2013, Robertson et al., 2012, Weber et al., 2012, Krahn et al., 2010, Krahn et al., 2010, Ramachandran et al., 2009, Djiane et al., 2005)

baz^815.8

(Jouette et al., 2019, Besson et al., 2015, Claret et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (23)

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