FlyBase Allele Report: Dmel\aPKC[k06403]

(Tian et al., 2023, Nagai et al., 2021, Holly et al., 2020, Bell et al., 2015, Guilgur et al., 2012, Weber et al., 2012, Kaplan et al., 2011, Ogawa et al., 2009, Ruiz-Canada et al., 2004, Nam and Choi, 2003, Wodarz et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity

(Kaplan et al., 2011, Rolls et al., 2003)

abnormal cell polarity | somatic clone

(Abdelilah-Seyfried et al., 2003)

abnormal cytokinesis | heat sensitive (with aPKC^ts)

(Guilgur et al., 2012)

abnormal locomotor behavior | larval stage

(Rolls et al., 2003)

abnormal locomotor behavior | larval stage (with Df(2R)Jp1)

(Rolls et al., 2003)

abnormal mitotic cell cycle

(Chabu and Doe, 2008)

abnormal neuroanatomy

(Lee et al., 2006)

abnormal neuroanatomy | somatic clone

(Rolls et al., 2003)

abnormal neurophysiology (with aPKC^Exc55)

(Ruiz-Canada et al., 2004)

abnormal planar polarity

(Kaplan et al., 2011)

abnormal planar polarity (with aPKC^ts)

(Guilgur et al., 2012)

abnormal size | heat sensitive (with aPKC^ts)

(Guilgur et al., 2012)

cell lethal | first instar larval stage | somatic clone

(Guilgur et al., 2012)

cell lethal | somatic clone

(Padash Barmchi et al., 2016)

decreased cell number | pupal stage | somatic clone

(Nam and Choi, 2003)

decreased cell number | somatic clone

(Rolls et al., 2003)

increased cell death | second instar larval stage | somatic clone

(Guilgur et al., 2012)

increased cell death | third instar larval stage | heat sensitive (with aPKC^ts)

(Guilgur et al., 2012)

lethal (with aPKC^psu265)

(Kim et al., 2009)

lethal (with aPKC^psu417)

(Kim et al., 2009)

lethal (with Df(2R)BSC330)

(Kahsai and Cook, 2018)

lethal (with Df(2R)Jp1)

(Kim et al., 2009)

lethal | heat sensitive (with aPKC^ts)

(Guilgur et al., 2012)

lethal | recessive

(Kim et al., 2009, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage

(Ruiz-Canada et al., 2004)

lethal - all die before end of second instar larval stage

(Rolls et al., 2003)

lethal - all die before end of second instar larval stage (with Df(2R)Jp1)

(Rolls et al., 2003)

lethal - all die before end of second instar larval stage | recessive

(Lee et al., 2006)

lethal - all die during embryonic stage (with Df(2R)Jp1)

(Wodarz et al., 2000)

lethal - all die during embryonic stage | recessive

(Wodarz et al., 2000)

partially lethal - majority die (with aPKC^psu69)

(Kim et al., 2009)

partially lethal - majority die (with aPKC^psu141)

(Kim et al., 2009)

some die during embryonic stage (with Df(2R)Jp1)

(Wodarz et al., 2000)

some die during embryonic stage | recessive

(Wodarz et al., 2000)

some die during larval stage

(Ruiz-Canada et al., 2004)

some die during second instar larval stage

(Rolls et al., 2003)

some die during second instar larval stage (with Df(2R)Jp1)

(Rolls et al., 2003)

some die during second instar larval stage | recessive

(Lee et al., 2006)

Phenotype Manifest In

actomyosin

(David et al., 2010)

bouton (with aPKC^Exc55)

(Ruiz-Canada et al., 2004)

egg chamber | germline clone

(Cox et al., 2001)

embryo | dorsal closure stage | heat sensitive (with aPKC^ts)

(Guilgur et al., 2012)

embryonic/first instar larval cuticle

(Wodarz et al., 2000)

embryonic/first instar larval cuticle (with Df(2R)Jp1)

(Wodarz et al., 2000)

embryonic/first instar larval cuticle | ventral

(Wodarz et al., 2000)

embryonic/larval cuticle | germline clone | maternal effect

(Kim et al., 2009)

embryonic head

(Wodarz et al., 2000)

epithelial cell

(Wodarz et al., 2000)

eye disc

(Rolls et al., 2003)

female germline cyst (with aPKC^ts)

(Guilgur et al., 2012)

follicle cell | ectopic | somatic clone

(Abdelilah-Seyfried et al., 2003)

follicle cell | germline clone

(Cox et al., 2001)

follicle cell | somatic clone

(Duchi et al., 2010, Kim et al., 2009, Abdelilah-Seyfried et al., 2003)

larval brain & neuroblast

(Lee et al., 2006)

larval neuroblast

(Chabu and Doe, 2008)

microtubule | somatic clone

(Duchi et al., 2010)

mushroom body & neuron | somatic clone

(Rolls et al., 2003)

nurse cell | germline clone

(Cox et al., 2001)

oocyte | germline clone

(Kim et al., 2009, Cox et al., 2001)

oocyte associated follicular epithelium | somatic clone

(Tian et al., 2023)

spermatid cyst

(Shao et al., 2023, Xu et al., 2014)

terminal tracheal cell | somatic clone

(Jones and Metzstein, 2011)

ventral denticle belt

(Kaplan et al., 2011)

wing disc | somatic clone

(Padash Barmchi et al., 2016)

wing disc | third instar larval stage | heat sensitive (with aPKC^ts)

(Guilgur et al., 2012)

zonula adherens | somatic clone

(Abdelilah-Seyfried et al., 2003)

Detailed Description

Statement

Reference

Homozygous clones are frequently not recovered in the wing disc and those that are recovered are small.

(Padash Barmchi et al., 2016)

Testes of heterozygous males sometimes contain misoriented spermatid cysts where the clustered spermatid nuclei are found apically (rather than basally).

(Xu et al., 2014)

aPKC^k06403 mutant wing disc clones cannot be detected at the first instar larval stage. However, clones can be seen when induction takes place at the second instar larval stage, and these are positive for apoptosis.

aPKC^ts/aPKC^k06403 flies are viable at the permissive temperature (25[o]C) and adult flies appear morphologically normal. However, females are sterile, with no larvae hatching from laid eggs even when crossed with wild type males. Germline cyst encapsulation phenotypes are seen immediately after the mesenchymal-to-epithelial transition in region 3 of the germarium in aPKC^ts/Df(2R)l4 mutant ovaries at the permissive temperature. The resulting follicular epithelium adherens junctions, apicobasal polarity and actin-myosin cytoskeleton appear normal. At the restrictive temperature (30[o]C) very few flies eclose from the pupal cases, with occasional escaper flies that exhibit abdominal dorsal closure defects. At 27-28[o]C (the semi-permissive temperature) viability is variable, but almost all flies show significant dorsal closure defects.

aPKC^ts/aPKC^k06403 third instar larval wing discs show a temperature sensitive induction of apoptosis. Whereas at the permissive temperatures (18[o]C and 25[o]C) there are low levels of cell extrusion and apoptosis, at the restrictive temperature (30[0]C) significantly higher levels of cell extrusion and apoptosis are seen, and the wings discs appear smaller than in controls. No phenotypes are seen in the heterozygous controls.

At both the permissive and restrictive temperatures there is a significant decrease in the proportion of aPKC^ts/aPKC^k06403 mutant wing disc cells with the correct planar spindle orientation compared to controls. The decrease is greater at 30[0]C than at 25[o]C.

Embryos maternally mutant for aPKC^ts/aPKC^k06403 fail to complete germ band extension (GBE), with a complete loss of epithelial integrity at the permissive temperature.

(Guilgur et al., 2012)

Homozygous tracheal terminal cells show a reduction in branching (primarily affecting class II and later-order branches) compared to wild type, a severe reduction in gas-filled lumen and an abnormal branch tip morphology.

(Jones and Metzstein, 2011)

aPKC^k06403 mutants display a mild defect in denticle belt formation.

(Kaplan et al., 2011)

Zygotic mutants for aPKC^k06403 complete embryogenesis due to maternally inherited gene product.

Compared with wild-type embryos, aPKC^k06403 mutants have significantly shorter lull times and significantly more frequent pulses for actomyosin assembly-disassembly cycles.

(David et al., 2010)

Microtubules are disorganised but are not lost in homozygous follicle cell clones. Piling up of follicle cells and stretching or gaps in the epithelium are seen.

(Duchi et al., 2010)

All aPKC^k06403 mutant adult Malpighian tubule renal and nephric stem cells (RNSCs) differentiate into renalcytes (RCs).

(Zeng et al., 2010)

Occasional aPKC^psu265/aPKC^k06403 and aPKC^psu417/aPKC^k06403 adult escapers are seen.

Embryos derived from homozygous female germ-line clones develop only scattered crumbs of cuticle. Epithelial cells in stage 7 embryos derived from homozygous female germ-line clones lose their columnar, monolayered organisation, rounding up and losing contact with each other.

Homozygous follicle cell clones show extensive multilayering of the epithelium.

An oocyte is specified in only 28% of germ-line clones.

(Kim et al., 2009)

Homozygous single neuroblast clones which are induced in first instar larvae and allowed to develop for 96 hours always contain a single large dpn-positive neuroblast (as do control single neuroblast clones).

Progression through mitosis is delayed in aPKC^k06403 larval neuroblasts compared to wild-type neuroblasts; the transit from nuclear envelope breakdown to anaphase onset is 17.84 +/- 4.52 minutes in the mutant neuroblasts compared to 7.76 +/- 2.04 minutes in wild-type neuroblasts.

(Chabu and Doe, 2008)

80% of homozygous female germline clones are arrested at stage 5 or 6 of oogenesis, but some germline clones do develop until stages 9 or 10.

(Tian and Deng, 2008)

Homozygous larvae have a reduced number of brain neuroblasts.

(Lee et al., 2006)

The transplantation of aPKC^k06403 larval neuroblast clones into adult hosts does not cause tumor formation.

(Caussinus and Gonzalez, 2005)

No aPKC^k06403 homozygous clones can be recovered in the eyes of aPKC^k06403 Scer\FRT; Scer\FLP1^ey.PN adults and only a few very small clones can be recovered in the eye imaginal disc.

(Djiane et al., 2005)

In aPKC^Exc55/aPKC^k06403 mutant animals, there is a decrease in the number of synaptic boutons at the neuromuscular junction (NMJ). Muscle size remains normal.

(Ruiz-Canada et al., 2004)

When somatic clones are made in the follicular epithelium cell polarity defects are seen. These are more severe at the poles of the egg chamber than in lateral positions where polarity is often normal. Zonula adherens is also compromised in clones. Mutant follicle cells are also multilayered. most strikingly at the anterior and posterior poles. When mutant clones are made in the border cells no migration defects are seen.

(Abdelilah-Seyfried et al., 2003)

Somatic clones of aPKC^k06403 in the pupal retina are very small - consisting of only a few cells, suggesting a role in proliferation and/or survival of retinal cells.

(Nam and Choi, 2003)

Stage 15 homozygous embryos have normal epithelial and neuroblast polarity. Homozygous aPKC^k06403 mutants die as late second instar larvae, they remain as second instar larvae for at least twice as long as normal. aPKC^k06403 mutant second larval instar eye imaginal discs are not organised into an epithelial monolayer and appear to have lost epithelial polarity. They are also smaller than in wild-type. aPKC^k06403 mutant clones exhibit a dramatic decrease in the number of neuronal progeny compared to wild-type. aPKC^k06403 mushroom body clones contain 75-125 neurons, consisting primarily of early-born γ neurons (in contrast to wild-type clones, which contain 300-400 neurons, consisting of early-born γ neurons as well as later-born α- and β-neurons).

(Rolls et al., 2003)

aPKC^k06403 germ-line clones fail to differentiate an oocyte as indicated by the presence of polyploid nurse cell nuclei in germ-line mutant egg chambers. Depletion of aPKC function from the follicle cells leads to their multilayering, which disrupts the normal partitioning of germ-line nuclei to successively mature egg chambers caused by mispositioning of mutant follicle cells. IN aPKC^k06403 mutant germ-line cysts ring canal distribution and spatioal distribution are indistinguishable from wild-type. No defects are seen in fusome morphology.

(Cox et al., 2001)

Homozygous aPKC^k06403 and aPKC^k06403/Df(2R)Jp1 embryos derived from heterozygous aPKC^k06403/+ females do not form cuticle because they die before cuticle secretion begins (most die before or during cellularisation). The embryos show multilayering of the epithelium at gastrulation, the cells are extremely irregular in shape and apico-basal polarity of the epithelium is lost. Embryos that contain a zygotic wild-type allele of aPKC and are derived from aPKC^k06403/+ females often die with characteristic head defects. At the anterior tip of the embryo, the epithelium is multilayered. Hemizygous embryos (that have a wild-type maternal contribution of aPKC) do not show obvious defects before germ band extension. The embryos form cuticles with severe head defects and large ventral holes, or lack ventral cuticle altogether.

(Wodarz et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

aPKC^k06403, arm^F1α has abnormal planar polarity phenotype, enhanceable by sgg^M11

(Kaplan et al., 2011)

aPKC^k06403, arm^F1α has abnormal cell polarity phenotype, enhanceable by sgg^M11

(Kaplan et al., 2011)

aPKC^k06403 has abnormal planar polarity phenotype, enhanceable by sgg^M11/arm^F1α

(Kaplan et al., 2011)

aPKC^k06403 has abnormal cell polarity phenotype, enhanceable by sgg^M11/arm^F1α

(Kaplan et al., 2011)

Suppressed by

Statement

Reference

aPKC^ts/aPKC^k06403 has increased cell death | heat sensitive phenotype, suppressible by raps[+]/pins¹⁹³

(Guilgur et al., 2012)

aPKC^k06403 has abnormal planar polarity phenotype, suppressible by arm^F1α

(Kaplan et al., 2011)

NOT suppressed by

Statement

Reference

aPKC^k06403 has abnormal cell polarity phenotype, non-suppressible by arm^F1α

(Kaplan et al., 2011)

Enhancer of

Statement

Reference

aPKC^k06403/aPKC[+] is an enhancer of abnormal cell polarity | embryonic stage | maternal effect phenotype of Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750

(Silver et al., 2019)

arm^F1α/aPKC^k06403 is an enhancer of abnormal planar polarity phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α/aPKC^k06403 is an enhancer of abnormal cell polarity phenotype of sgg^M11

(Kaplan et al., 2011)

sgg^M11/aPKC^k06403 is an enhancer of abnormal planar polarity phenotype of arm^F1α

(Kaplan et al., 2011)

sgg^M11/aPKC^k06403 is an enhancer of abnormal cell polarity phenotype of arm^F1α

(Kaplan et al., 2011)

NOT Enhancer of

Statement

Reference

aPKC^k06403/aPKC[+] is a non-enhancer of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Suppressor of

Statement

Reference

aPKC^k06403/aPKC[+] is a suppressor | partially of abnormal cell migration | oogenesis phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

aPKC^k06403/aPKC[+] is a suppressor of abnormal neuroanatomy | larval stage phenotype of l(2)gl³³⁴/l(2)gl³⁶⁴⁴

(Haenfler et al., 2012)

aPKC^k06403/aPKC[+] is a suppressor of abnormal neuroanatomy | somatic clone phenotype of Zif^1L15

(Chang et al., 2010)

aPKC^k06403/aPKC[+] is a suppressor of increased cell number | somatic clone phenotype of tws^j11C8

(Chabu and Doe, 2009)

aPKC^k06403/aPKC[+] is a suppressor of abnormal neuroanatomy | adult stage phenotype of DCTN1-p150^Δ.UAS, Scer\GAL4^shot-OK307

(Ma et al., 2009)

aPKC^k06403/aPKC[+] is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

aPKC^k06403/aPKC[+] is a non-suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

aPKC^k06403 is a non-suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Other

Statement

Reference

PKD^cl4, aPKC^k06403 has viable phenotype

(Maier et al., 2019)

PKD^cl4, aPKC^k06403 has partially lethal - majority live phenotype

(Maier et al., 2019)

aPKC^k06403/Df(2R)BSC330, baz^815-8 has viable phenotype

(Besson et al., 2015)

aPKC^k06403/Df(2R)BSC330, baz^815-8 has fertile phenotype

(Besson et al., 2015)

aPKC^k06403/aPKC[+], wts^x1 has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

aPKC^k06403, arm^F1α has abnormal cell polarity phenotype

(Kaplan et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

aPKC^k06403 has spermatid cyst phenotype, enhanceable by eIF4E5^B8a

(Shao et al., 2023)

aPKC^k06403 has spermatid cyst phenotype, enhanceable by eIF4E5^B8b

(Shao et al., 2023)

aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype, enhanceable by Scer\GAL4^Act.PU/Egfr^1.A887T.UAS

(Tian et al., 2023)

aPKC^k06403 has spermatid cyst phenotype, enhanceable by orb2[+]/orb2³⁶

(Xu et al., 2014)

aPKC^k06403, arm^F1α has ventral denticle belt phenotype, enhanceable by sgg^M11

(Kaplan et al., 2011)

aPKC^k06403 has ventral denticle belt phenotype, enhanceable by sgg^M11/arm^F1α

(Kaplan et al., 2011)

NOT Enhanced by

Statement

Reference

aPKC^k06403, zip¹ has embryonic/first instar larval cuticle phenotype, non-enhanceable by par-6^Δ226

(David et al., 2010)

Suppressed by

Statement

Reference

aPKC^ts/aPKC^k06403 has wing disc | third instar larval stage | heat sensitive phenotype, suppressible by raps[+]/pins¹⁹³

(Guilgur et al., 2012)

aPKC^ts/aPKC^k06403 has embryo | dorsal closure stage | heat sensitive phenotype, suppressible by raps[+]/pins¹⁹³

(Guilgur et al., 2012)

aPKC^k06403 has ventral denticle belt phenotype, suppressible by arm^F1α

(Kaplan et al., 2011)

Enhancer of

Statement

Reference

aPKC^k06403 is an enhancer of spermatid cyst phenotype of eIF4E5^B8a

(Shao et al., 2023)

aPKC^k06403 is an enhancer of spermatid cyst phenotype of eIF4E5^B8b

(Shao et al., 2023)

aPKC^k06403/aPKC[+] is an enhancer of embryonic epidermis | maternal effect phenotype of Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}, cyst^HMS01750

(Silver et al., 2019)

arm^F1α/aPKC^k06403 is an enhancer of ventral denticle belt phenotype of sgg^M11

(Kaplan et al., 2011)

sgg^M11/aPKC^k06403 is an enhancer of ventral denticle belt phenotype of arm^F1α

(Kaplan et al., 2011)

aPKC^k06403 is an enhancer of embryonic/first instar larval cuticle phenotype of zip¹

(David et al., 2010)

aPKC^k06403/aPKC[+] is an enhancer of embryonic/larval cuticle phenotype of baz⁴

(Shao et al., 2010)

aPKC^k06403 is an enhancer of neuroblast | third instar larval stage phenotype of l(2)gl⁴

(Rolls et al., 2003)

NOT Enhancer of

Statement

Reference

aPKC^k06403/aPKC[+] is a non-enhancer of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

aPKC^k06403 is a non-enhancer of embryonic/first instar larval cuticle phenotype of par-6^Δ226, zip¹

(David et al., 2010)

Suppressor of

Statement

Reference

aPKC^k06403/aPKC[+] is a suppressor | partially of border follicle cell phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

aPKC^k06403/aPKC[+] is a suppressor | partially of egg chamber phenotype of slmb^9H4-17/slmb⁸, slmb^L.hs

(Morais-de-Sá et al., 2014)

aPKC^k06403/aPKC[+] is a suppressor of type II neuroblast | increased number | larval stage phenotype of l(2)gl³³⁴/l(2)gl³⁶⁴⁴

(Haenfler et al., 2012)

aPKC^k06403/aPKC[+] is a suppressor of intermediate neuronal progenitor | increased number | larval stage phenotype of l(2)gl³³⁴/l(2)gl³⁶⁴⁴

(Haenfler et al., 2012)

aPKC^k06403/aPKC[+] is a suppressor of neuroblast | increased number | somatic clone phenotype of Zif^1L15

(Chang et al., 2010)

aPKC^k06403/aPKC[+] is a suppressor | partially of embryonic/larval optic lobe phenotype of Df(3R)Exel6265/tws^j11C8

(Chabu and Doe, 2009)

aPKC^k06403/aPKC[+] is a suppressor | partially of larval neuroblast | increased number phenotype of Df(3R)Exel6265/tws^j11C8

(Chabu and Doe, 2009)

aPKC^k06403/aPKC[+] is a suppressor of larval neuroblast | increased number | somatic clone phenotype of tws^j11C8

(Chabu and Doe, 2009)

aPKC^k06403/aPKC[+] is a suppressor of giant fiber neuron phenotype of DCTN1-p150^Δ.UAS, Scer\GAL4^shot-OK307

(Ma et al., 2009)

aPKC^k06403 is a suppressor of larval brain & neuroblast | supernumerary phenotype of l(2)gl³³⁴

(Lee et al., 2006)

aPKC^k06403/aPKC[+] is a suppressor of NMJ bouton | larval stage phenotype of Fmr1³

(Zarnescu et al., 2005)

aPKC^k06403/aPKC[+] is a suppressor of synapse & larval neuromuscular junction phenotype of Fmr1³

(Zarnescu et al., 2005)

aPKC^k06403/aPKC[+] is a suppressor | partially of eye phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

aPKC^k06403/aPKC[+] is a suppressor | partially of ommatidium phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

aPKC^k06403/aPKC[+] is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

aPKC^k06403/aPKC[+] is a suppressor of wing disc phenotype of l(2)gl³³⁴

(Rolls et al., 2003)

aPKC^k06403/aPKC[+] is a suppressor of neuroblast & larval brain & third instar larva phenotype of l(2)gl³³⁴

(Rolls et al., 2003)

aPKC^k06403/aPKC[+] is a suppressor of ventral thoracic disc & epithelial cell phenotype of l(2)gl³³⁴

(Rolls et al., 2003)

aPKC^k06403/aPKC[+] is a suppressor of leg disc phenotype of l(2)gl³³⁴

(Rolls et al., 2003)

aPKC^k06403/aPKC[+] is a suppressor of dorsal mesothoracic disc & epithelial cell phenotype of l(2)gl³³⁴

(Rolls et al., 2003)

aPKC^k06403/aPKC[+] is a suppressor | partially of larval brain | third instar larval stage phenotype of l(2)gl³³⁴/l(2)gl⁴

(Rolls et al., 2003)

NOT Suppressor of

Statement

Reference

aPKC^k06403/aPKC[+] is a non-suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

aPKC^k06403 is a non-suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Other

Statement

Reference

Egfr^1.A887T.UAS, Scer\GAL4^Act.PU, aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Egfr^1.A887T.UAS, Rok^CAT-KG.UAS, Scer\GAL4^Act.PU, aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Rok^CAT-KG.UAS, Scer\GAL4^Act.PU, aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Scer\GAL4^Act.PU, aPKC^k06403, sqh^{T20A.S21A.cKa.UASp} has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Egfr^GD1654, Scer\GAL4^Act.PU, aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Scer\GAL4^Act.PU, aPKC^k06403, l(2)gl^GD4047 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

aPKC^k06403/aPKC[+], wts^x1 has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

aPKC^k06403, arm^F1α has ventral denticle belt phenotype

(Kaplan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from aPKC^k06403, Df(2L)BSC301 double heterozygous females do not show planar polarity defects (i.e. in Baz localization pattern).

(Silver et al., 2019)

The proportion of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage is significantly increased in wts^x1/+;aPKC^k06403/+ double heterozygous embryos compared to either of the single heterozygotes or wild type.

(Keder et al., 2015)

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of aPKC^k06403.

(Zaessinger et al., 2015)

Lowering aPKC levels, by a aPKC^k06403 heterozygous background, partially suppresses the multilayering cell phenotype found in slmb^L.hs;slmb⁸/slmb^9H4-17 mutants.

(Morais-de-Sá et al., 2014)

orb2³⁶/+ enhances the frequency of testes containing misoriented spermatid cysts in aPKC^k06403/+ males.

(Xu et al., 2014)

raps¹⁹³ dominantly suppresses the temperature sensitive induction of apoptosis seen in aPKC^ts/aPKC^k06403 third instar larval wing discs at the semi-permissive temperature (28[o]C). However, this suppression does not occur at the restrictive temperature (30[o]C).

raps¹⁹³ dominantly suppresses the abdominal dorsal closure defects seen in aPKC^ts/aPKC^k06403 mutant flies at the semi-permissive temperature (28[o]C). However, this suppression did not occur at the restrictive temperature (30[o]C).

(Guilgur et al., 2012)

aPKC^k06403/+ suppresses the increased number of type II neuroblasts and intermediate neural progenitor cells seen in l(2)gl³³⁴/l(2)gl³⁶⁴⁴ larval brains.

(Haenfler et al., 2012)

arm^F1α; aPKC^k06403 double mutants maintain some apical-basal polarity patterning, and while cell shapes are somewhat disrupted, cells appear to align in rows.

arm^F1α; sgg^M11; aPKC^k06403 triple mutants exhibit significant denticle and cell disruption. Specifically, proper cell shapes and cell alignment are lost, and instead of organization into even rows, cells curved and formed denticle swirls.

(Kaplan et al., 2011)

The neuroblast overgrowth phenotype seen in Zif^1L15 mutant clones is significantly suppressed in a aPKC^k06403 heterozygous background.

(Chang et al., 2010)

aPKC^k06403, zip¹ double mutants display a prominent dorsal hoe merged with a large head hole indicating enhancement of the zip¹ embryonic phenotype. par-6^Δ226, zip¹, aPKC^k06403 triple mutants show similar enhancement.

(David et al., 2010)

The cuticle phenotype of dead embryos derived from baz⁴/+ embryos derived from a cross of baz⁴/+ females to wild-type males is enhanced if the females also carry one copy of aPKC^k06403.

(Shao et al., 2010)

Hyperplasia in adult Malpighian tubule clones expressing Ras85D^V12.Scer\UAS (using the MARCM system, under the control of Scer\GAL80^αTub84B.PL and Scer\GAL4^{Scer\FRT.Act5C}) is not suppressed by making them also mutant for aPKC^k06403.

(Zeng et al., 2010)

Larval tws^j11C8 mutant clones in an aPKC^k06403/+ background contain an intermediate number of neuroblasts per clone, partially rescuing the single cell tws^j11C8 clone phenotype.

The third instar optic lobe of tws^j11C8/Df(3R)Exel6265 in a aPKC^k06403/+ background have a partially reduced number of optic lobe neuroblasts, partially rescuing the tws^j11C8/Df(3R)Exel6265 phenotype.

(Chabu and Doe, 2009)

The giant fiber axon defects seen in adults expressing Gl^Δ.Scer\UAS under the control of Scer\GAL4^A307 are partially suppressed by aPKC^k06403/+.

(Ma et al., 2009)

The reduced size seen in l(2)gl⁴ L3 larval neuroblasts is not suppressed by aPKC^k06403/aPKC^k06403 and may in fact be slightly enhanced. l(2)gl³³⁴ aPKC^k06403/l(2)gl³³⁴ + larvae exhibit essentially normal leg and wing imaginal disc morphology including disc size, columnar cell shape and an approximately normal apical membrane domain, at the early third instar stage. The increase in L3 larval brain volume seen in l(2)gl³³⁴/l(2)gl⁴ animals is partially suppressed by aPKC^k06403/+. The increase in neuroblast number seen in homozygous l(2)gl³³⁴ third larval instar brains is partially suppressed by aPKC^k06403/+. aPKC^k06403 heterozygosity cannot suppress the l(2)gl⁴ "small neuroblast" phenotype.

(Rolls et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

aPKC^SG58

(Ma et al., 2009)

Rescued by

aPKC^k06403 is rescued by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

aPKC^k06403 is rescued by aPKC^UAS.cBa/Scer\GAL4^da.G32

(Kaplan et al., 2011)

aPKC^k06403 is rescued by aPKC^{S330A.T422A.UAS}/Scer\GAL4^da.G32

(Kaplan et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10622

y¹ w^67c23; P{lacW}aPKC^k06403/CyO

Kyoto

111194

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}aPKC^k06403 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Excision of the P{lacW} element can revert the lethality of aPKC^k06403, indicating that the P{lacW} insertion is responsible for the aPKC^k06403 phenotype.

(Wodarz et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

DaPKC^k06403

(Dong et al., 2020, Zeng et al., 2010, Abdelilah-Seyfried et al., 2003, Cox et al., 2001, Wodarz et al., 2000)

aPKC-zeta^k06403

(Zarnescu et al., 2005)

aPKC⁰⁶⁴⁰³

(Haenfler et al., 2012, Chang et al., 2010, Ma et al., 2009, Wang et al., 2009)

aPKC⁶⁴⁰³

(Fletcher et al., 2012)

aPKC^K06304

(Nam and Choi, 2003)

aPKC^K06403

(Holly et al., 2020, Ventura et al., 2020, Silver et al., 2019, Calero-Cuenca et al., 2016, Bell et al., 2015, Keder et al., 2015, Jiang et al., 2014, Leibfried et al., 2013, Sotillos et al., 2013, David et al., 2010, Morais-de-Sá et al., 2010, Shao et al., 2010, Kaplan et al., 2009)

aPKC^k06043

(Chen et al., 2018)

aPKC^k0643

(Chabu and Doe, 2008)

aPKC^l(2)K06403

(Sotillos et al., 2008)

aPKC^l(2)k06403

(Wang and Riechmann, 2007)

aPKCK06403

(Jones et al., 2023)

apkc⁰⁶⁴⁰³

(Xu et al., 2014)

apkc^K06403

(Tian and Deng, 2008)

apkc^k060430

(Besson et al., 2015)

apkc^k06403

(Tian et al., 2023, Hannaford et al., 2018, Xu et al., 2014, Guilgur et al., 2012)

daPKC^k06403

(Zarnescu et al., 2005)

dapkc⁰⁶⁴⁰³

(Ruiz-Canada et al., 2004)

l(2)k06403

(Ruiz-Canada et al., 2004, Spradling et al., 1999)

l(2)k06403^k06403

Name Synonyms

Secondary FlyBase IDs

References (74)

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