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General Information
Symbol
Dmel\l(2)gl334
Species
D. melanogaster
Name
FlyBase ID
FBal0009238
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
lgl334, 334, l(2)glU334
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Distal portion of l(2)gl and telomeric flanking sequences are deleted.

Deletion of sequences from 0 to 10kb (coordinates with respect to proximal end of a cloned l(2)gl region, close to the tip of 2L).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Scer\GAL4hh.PU>l(2)glASA.Scer\UAS.T:Avic\GFP rescues the cell polarity defects in l(2)gl4/l(2)gl334 mutant wing disc epithelia, but reveals spindle orientation defects.

Expression of Scer\GAL4hh.PU>l(2)glScer\UAS.T:Uuuu\Myr-Unk rescues the cell polarity defects in l(2)gl4/l(2)gl334 mutant wing disc epithelia, but reveals spindle orientation defects.

At 24 hours after clone induction, type I and II neuroblast clones in l(2)gl334/l(2)gl3644 larval brains contain a single neuroblast, as occurs in wild type. However, at 48 hours after clone induction, the mutant type I and II neuroblast clones contain supernumerary neuroblasts.

Intermediate neural progenitor cell derived clones in l(2)gl334/l(2)gl3644 larval brains contain multiple supernumerary type II neuroblasts.

l(2)gl334/l(2)gl3644 larval brains contain an increased number of type II neuroblasts and intermediate neural progenitor cells compared to wild type.

Mutants show an increased number of brain neuroblasts at 96 hours after larval hatching compared to controls. Mutant neuroblast clones in the larval brain often contain two neuroblasts, and can contain up to six neuroblasts, in contrast to control clones which contain a single neuroblast.

l(2)gl4/l(2)gl334 L3 larval brains are larger in volume (65% larger) than l(2)gl334/+ brains. l(2)gl334/l(2)gl334 L3 larval brains possess 136 +/- 12 neuroblasts compared to a l(2)gl334/+ value of 92 +/- 6. Homozygous l(2)gl334 L3 larval imaginal discs are enlarged compared to l(2)gl334 heterozygous discs. They don't exhibit a monolayer at L2/L3 larval stages and have a ~49% increase in the number of neuroblasts compared to controls. l(2)gl334 mutant third instar leg and wing imaginal discs are highly enlarged and disorganised, and often fuse with each other or nearby tissues. At the cellular level, mutant epithelial cells are round instead of columnar, with an expanded apical membrane domain.

Salivary glands isolated from 8 day old third instar l(2)gl334/l(2)gl4 larvae and maintained in Schneider culture medium for 24 hours are considerably reduced in size compared to wild-type controls and have a "bunch of grapes" shape. The cells of the mutant glands are nearly spherical in form with a reduced surface of cell-to-cell contact. Administration of 10[-5]M 20-hydroxyecdysone fails to induce the histolysis of the mutant glands. No sign of vacuolisation or abnormal pattern of organelle distribution can be detected in treated mutant salivary gland cells and the overall organisation of the glands and structure of the cells is similar to those of untreated mutant glands.

l(2)gl558/l(2)gl334 transheterozygotes show temperature-sensitive viability. The temperature-sensitive period is biphasic; the first phase is embryonic and the second phase is in the third larval-prepupal stage.

Some Df(2L)U558/l(2)gl334 flies are viable. The viability is affected by temperature; the temperature sensitive period is diphasic at 17oC and 29oC.

18.4% of mutants die as larvae, 81.6% as pupae. Weak allele: brain and discs are small and rudimentary and grow slowly in transplants.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

l(2)gl334 has larval brain & neuroblast | supernumerary phenotype, suppressible by aPKCk06403

l(2)gl334 has wing disc phenotype, suppressible by aPKCk06403/aPKC[+]

l(2)gl334 has neuroblast & larval brain & third instar larva phenotype, suppressible | partially by aPKCk06403/aPKC[+]

l(2)gl334 has ventral thoracic disc & epithelial cell phenotype, suppressible by aPKCk06403/aPKC[+]

l(2)gl334 has ventral thoracic disc phenotype, suppressible by aPKCk06403/aPKC[+]

l(2)gl334 has dorsal mesothoracic disc & epithelial cell phenotype, suppressible by aPKCk06403/aPKC[+]

l(2)gl334 has phenotype, suppressible by Dpse\l(2)gl+t12.2

NOT suppressed by
Suppressor of
Statement
Reference

l(2)gl334/l(2)gl[+] is a suppressor | partially of ommatidium phenotype of Fmr1sev.PW

l(2)gl334/l(2)gl[+] is a suppressor | partially of retina phenotype of Fmr1sev.PW

l(2)gl334/l(2)gl[+] is a suppressor | partially of eye phenotype of Fmr1sev.PW

Additional Comments
Genetic Interactions
Statement
Reference

aPKCk06403/+ suppresses the increased number of type II neuroblasts and intermediate neural progenitor cells seen in l(2)gl334/l(2)gl3644 larval brains.

The formation of supernumerary type I neuroblasts which is seen in l(2)gl334/l(2)gl3644 larval brains is suppressed by expression of either numbScer\UAS.T:Ivir\HA1, numbΔN.Scer\UAS.hs.T:Hsap\MYC, numbS2A.Scer\UAS.T:Ivir\HA1, numbS2D.Scer\UAS.T:Ivir\HA1 or numbΔC.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ase.neuro, but is not suppressed by expression of either numbΔPTB.Scer\UAS.T:Ivir\HA1 or numbΔAB.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ase.neuro.

The formation of supernumerary type I neuroblasts which is seen in l(2)gl334/l(2)gl3644 larval brains is suppressed by co-expression of numbΔC.Scer\UAS.T:Ivir\HA1, but is not suppressed by co-expression of either numbΔAB.Scer\UAS.T:Ivir\HA1 or numbΔPTB.Scer\UAS.T:Ivir\HA1.

The brains of l(2)gl334 rapsP62 double mutant larvae contain large numbers of ectopic, proliferating, self-renewing neuroblasts.

l(2)gl334 aPKCk06403/l(2)gl334 + larvae exhibit essentially normal leg and wing imaginal disc morphology including disc size, columnar cell shape and an approximately normal apical membrane domain, at the early third instar stage. The increase in L3 larval brain volume seen in l(2)gl334/l(2)gl4 animals is partially suppressed by aPKCk06403/+. The increase in neuroblast number seen in homozygous l(2)gl334 third larval instar brains is partially suppressed by aPKCk06403/+.

Xenogenetic Interactions
Statement
Reference

Mutant phenotype completely rescued by Dpse\l(2)gl+t12.2.

Complementation and Rescue Data
Comments

Expression of Scer\GAL4hh.PU>l(2)glScer\UAS.P\T.T:Avic\GFP rescues the cell polarity defects in l(2)gl4/l(2)gl334 mutants.

Expression of Scer\GAL4hh.PU>l(2)glASA.Scer\UAS.T:Avic\GFP rescues the cell polarity defects in l(2)gl4/l(2)gl334 mutants and l(2)gl4 mutant somatic clones, but reveals spindle orientation defects.

Expression of Scer\GAL4hh.PU>l(2)glScer\UAS.T:Uuuu\Myr-Unk rescues the cell polarity defects in l(2)gl4/l(2)gl334 mutants and l(2)gl4 mutant somatic clones, but reveals spindle orientation defects.

Mutant phenotype can be completely rescued by l(2)gl+t13.1.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Isolated from: Uman', Ukraine population.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (19)