FlyBase Allele Report: Dmel\arm[F1α]

General Information

Symbol

Dmel\arm^F1α

Species

D. melanogaster

Name

FlyBase ID

FBal0152118

Feature type

allele

Associated gene

Dmel\arm

Associated Insertion(s)

Carried in Construct

Also Known As

arm^F1a

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Tolwinski et al., 2003)

loss of function allele

(Tolwinski and Wieschaus, 2004)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: R394H.

(Kaplan et al., 2011)

Nucleotide substitution: G2990A.

(Tolwinski and Wieschaus, 2004)

Amino acid replacement: R294H.

(Tolwinski et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:1,895,604..1,895,604 [-]

Nucleotide change:

G1895604A

Reported nucleotide change:

G2990A

Amino acid change:

Reported amino acid change:

R294H

Comment:

The reported nucleotide and amino acid substitution positions for arm[F1alpha do not match. The reported position of the nucleotide substitution was mapped since it is consistent with other mutation locations in the same report.

(Tolwinski and Wieschaus, 2004, Tolwinski et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity

(Kaplan et al., 2011)

abnormal planar polarity

(Kaplan et al., 2011)

lethal - all die before end of pupal stage | recessive

(Tolwinski et al., 2003)

segment polarity phenotype | embryonic stage | maternal effect

(Mannava and Tolwinski, 2015)

some die during pupal stage | recessive

(Tolwinski et al., 2003)

Phenotype Manifest In

denticle | germline clone

(Tolwinski and Wieschaus, 2004)

denticle field | embryonic stage | maternal effect

(Mannava and Tolwinski, 2015)

embryonic/first instar larval cuticle | germline clone

(Tolwinski and Wieschaus, 2004)

embryonic/larval cuticle | embryonic stage | maternal effect

(Mannava and Tolwinski, 2015)

ventral denticle belt

(Kaplan et al., 2011)

Detailed Description

Statement

Reference

Maternally and zygotically mutant arm^F1α embryos (coming from mothers whose whole germline constitutes from arm^F1α mutant cells only - created by the OvoD germline clone technique) show a segment polarity phenotype and no naked cuticle between denticle belts on the ventral epidermis (resulting in a lawn of denticles).

(Mannava and Tolwinski, 2015)

arm^F1α mutants exhibit disruption of denticle belt patterning, with some patterning being maintained while cell shape is only mildly disrupted. Cells are rectangular and the asymmetry of markers is moderately maintained.

(Kaplan et al., 2011)

Embryos mutant for arm^F1α both maternally and zygotically display loss of naked regions of the embryonic cuticle.

(Kaplan et al., 2009)

Mutant embryos (created using germ-line clones) exhibit a weak ectopic denticle phenotype.

When arm^{Δ.Scer\UAS.T:Ivir\HA1} is driven in a arm^F1α germ-line clone background, a cuticle pattern with periodic denticle belts and regions of intervening naked cuticle are formed.

(Tolwinski and Wieschaus, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

arm^F1α has abnormal cell polarity phenotype, enhanceable by sgg^M11/aPKC^k06403

(Kaplan et al., 2011)

arm^F1α, sgg^M11 has abnormal planar polarity phenotype, enhanceable by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

arm^F1α, sgg^M11 has abnormal cell polarity phenotype, enhanceable by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

aPKC^k06403, arm^F1α has abnormal planar polarity phenotype, enhanceable by sgg^M11

(Kaplan et al., 2011)

aPKC^k06403, arm^F1α has abnormal cell polarity phenotype, enhanceable by sgg^M11

(Kaplan et al., 2011)

arm^F1α has abnormal planar polarity phenotype, enhanceable by sgg^M11/aPKC^k06403

(Kaplan et al., 2011)

NOT Enhanced by

Statement

Reference

arm^F1α has segment polarity phenotype | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^{UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has segment polarity phenotype | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has segment polarity phenotype | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has abnormal planar polarity phenotype, non-enhanceable by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

arm^F1α has abnormal cell polarity phenotype, non-enhanceable by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

NOT suppressed by

Statement

Reference

arm^F1α has segment polarity phenotype | maternal effect | embryonic stage phenotype, non-suppressible by sgg^{UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has segment polarity phenotype | maternal effect | embryonic stage phenotype, non-suppressible by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has segment polarity phenotype | maternal effect | embryonic stage phenotype, non-suppressible by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has abnormal planar polarity phenotype, non-suppressible by sgg^M11

(Kaplan et al., 2011)

arm^F1α has abnormal cell polarity phenotype, non-suppressible by sgg^M11

(Kaplan et al., 2011)

arm^F1α has abnormal planar polarity phenotype, non-suppressible by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

arm^F1α has abnormal cell polarity phenotype, non-suppressible by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

Enhancer of

Statement

Reference

arm^F1α/aPKC^k06403 is an enhancer of abnormal planar polarity phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α/aPKC^k06403 is an enhancer of abnormal cell polarity phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.UAS is an enhancer of abnormal planar polarity phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.UAS is an enhancer of abnormal cell polarity phenotype of sgg^M11

(Kaplan et al., 2011)

sgg^M11/arm^F1α is an enhancer of abnormal planar polarity phenotype of aPKC^k06403

(Kaplan et al., 2011)

sgg^M11/arm^F1α is an enhancer of abnormal cell polarity phenotype of aPKC^k06403

(Kaplan et al., 2011)

Suppressor of

Statement

Reference

arm^F1α is a suppressor of abnormal planar polarity phenotype of aPKC^k06403

(Kaplan et al., 2011)

arm^F1α is a suppressor of abnormal planar polarity phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α is a suppressor of abnormal cell polarity phenotype of sgg^M11

(Kaplan et al., 2011)

NOT Suppressor of

Statement

Reference

arm^F1α is a non-suppressor of abnormal cell polarity phenotype of aPKC^k06403

(Kaplan et al., 2011)

Other

Statement

Reference

aPKC^k06403, arm^F1α has abnormal cell polarity phenotype

(Kaplan et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

arm^F1α, sgg^M11 has ventral denticle belt phenotype, enhanceable by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

aPKC^k06403, arm^F1α has ventral denticle belt phenotype, enhanceable by sgg^M11

(Kaplan et al., 2011)

arm^F1α has ventral denticle belt phenotype, enhanceable by sgg^M11/aPKC^k06403

(Kaplan et al., 2011)

NOT Enhanced by

Statement

Reference

arm^F1α has embryonic/larval cuticle | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^{UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has denticle field | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^{UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has embryonic/larval cuticle | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has denticle field | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has embryonic/larval cuticle | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has denticle field | embryonic stage | maternal effect phenotype, non-enhanceable by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has ventral denticle belt phenotype, non-enhanceable by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

arm^F1α has embryonic/first instar larval cuticle | maternal effect phenotype, non-enhanceable by sgg^M11

(Kaplan et al., 2009)

arm^F1α has embryonic/first instar larval cuticle | maternal effect phenotype, non-enhanceable by l(2)gl⁴

(Kaplan et al., 2009)

arm^F1α has embryonic/first instar larval cuticle | germline clone phenotype, non-enhanceable by Scer\GAL4^arm.PS/Cby^RNAi.UAS.WIZ

(Tolwinski and Wieschaus, 2004)

NOT suppressed by

Statement

Reference

arm^F1α has embryonic/larval cuticle | maternal effect | embryonic stage phenotype, non-suppressible by sgg^{UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has embryonic/larval cuticle | maternal effect | embryonic stage phenotype, non-suppressible by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has embryonic/larval cuticle | maternal effect | embryonic stage phenotype, non-suppressible by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has denticle field | maternal effect | embryonic stage phenotype, non-suppressible by sgg^{UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has denticle field | maternal effect | embryonic stage phenotype, non-suppressible by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has denticle field | maternal effect | embryonic stage phenotype, non-suppressible by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^F1α has ventral denticle belt phenotype, non-suppressible by sgg^M11

(Kaplan et al., 2011)

arm^F1α has ventral denticle belt phenotype, non-suppressible by Scer\GAL4^da.G32/aPKC^ΔN.UAS

(Kaplan et al., 2011)

arm^F1α has embryonic/first instar larval cuticle | maternal effect phenotype, non-suppressible by l(2)gl⁴

(Kaplan et al., 2009)

arm^F1α has embryonic/first instar larval cuticle | maternal effect phenotype, non-suppressible by sgg^M11

(Kaplan et al., 2009)

arm^F1α has embryonic/first instar larval cuticle | germline clone phenotype, non-suppressible by Scer\GAL4^arm.PS/Cby^RNAi.UAS.WIZ

(Tolwinski and Wieschaus, 2004)

Enhancer of

Statement

Reference

arm^F1α/aPKC^k06403 is an enhancer of ventral denticle belt phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.UAS is an enhancer of ventral denticle belt phenotype of sgg^M11

(Kaplan et al., 2011)

sgg^M11/arm^F1α is an enhancer of ventral denticle belt phenotype of aPKC^k06403

(Kaplan et al., 2011)

NOT Enhancer of

Statement

Reference

sgg^M11/arm^F1α is a non-enhancer of embryonic/first instar larval cuticle phenotype of l(2)gl⁴

(Kaplan et al., 2009)

Suppressor of

Statement

Reference

arm^F1α is a suppressor of ventral denticle belt phenotype of aPKC^k06403

(Kaplan et al., 2011)

arm^F1α is a suppressor of ventral denticle belt phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α is a suppressor of embryonic/first instar larval cuticle | germline clone | rescuable maternal effect phenotype of sgg^M11

(Tolwinski et al., 2003)

arm^F1α is a suppressor of ventral denticle belt | germline clone | rescuable maternal effect phenotype of sgg^M11

(Tolwinski et al., 2003)

NOT Suppressor of

Statement

Reference

sgg^M11/arm^F1α is a non-suppressor of embryonic/first instar larval cuticle phenotype of l(2)gl⁴

(Kaplan et al., 2009)

Other

Statement

Reference

aPKC^k06403, arm^F1α has ventral denticle belt phenotype

(Kaplan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The segment polarity phenotype and the lack of naked cuticle between denticle belts, resulting in a lawn of denticles on the ventral epidermis, characteristic for maternally and zygotically mutant arm^F1α embryos is not modified by Scer\GAL4^arm.PU-driven expression of any of the following: sgg^{Scer\UAS.T:Myr-Src64B,T:Ivir\HA1}, sgg^{KK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1} or sgg^{Scer\UAS.T:Ivir\HA1}.

(Mannava and Tolwinski, 2015)

A sgg^M11 mutant background fails to affect the epithelial patterning defects found in arm^F1α mutants.

arm^F1α; aPKC^k06403 double mutants maintain some apical-basal polarity patterning, and while cell shapes are somewhat disrupted, cells appear to align in rows.

Expression of aPKC^ΔN.Scer\UAS in arm^F1α mutant embryos does not affect on denticle organisation.

arm^F1α; sgg^M11; aPKC^k06403 triple mutants exhibit significant denticle and cell disruption. Specifically, proper cell shapes and cell alignment are lost, and instead of organization into even rows, cells curved and formed denticle swirls.

(Kaplan et al., 2011)

Cuticles of embryos double mutant for arm^F1α maternally and zygotically and l(2)gl⁴ zygotically resemble arm^F1α single mutant embryos.

Cuticles of embryos double mutant maternally and zygotically for arm^F1α and sgg^M11 resemble that of arm^F1α single mutant embryos.

Embryos triple mutant for sgg^M11 both maternally and zygotically and arm^F1α both maternally and zygotically and l(2)gl⁴ zygotically show a cuticle phenotype that is similar to that of zygotic l(2)gl⁴ single mutants.

(Kaplan et al., 2009)

When Cby^{dsRNA.Scer\UAS.WIZ} is driven by Scer\GAL4^arm.PS in a arm^F1α germ-line clone background, the ectopic denticle phenotype is suppressed. arm^F1α, arm^{Δ.Scer\UAS.T:Ivir\HA1}, Cby^{dsRNA.Scer\UAS.WIZ} (driven by Scer\GAL4^arm.PS) germ-line clones exhibit a naked cuticle phenotype. When Cby^{dsRNA.Scer\UAS.WIZ} is driven by Scer\GAL4^arm.PS in a arm^{Δ.Scer\UAS.T:Ivir\HA1} germ-line clone background, the ectopic denticle phenotype is enhanced.

Expression of Cby^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^arm.PS has no effect on the cuticle phenotype of embryos derived from arm^F1α germ-line clones.

Co-expression of Cby^{dsRNA.Scer\UAS.WIZ} in a arm^F1α, arm^{Δ.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^arm.PS background leads to increased naked cuticle compared to arm^F1α, arm^{Δ.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^arm.PS embryos.

(Tolwinski and Wieschaus, 2004)

The loss of denticle belts in the cuticle made by sgg^M11/sgg^M11 maternal/zygotic mutant embryos, is suppressed by maternal/zygotic arm^F1α/arm^F1α (a weaker suppression is seen when arm⁸ is used instead). When these double mutant embryos are also zygotically mutant for wg^l-8, they make little naked cuticle, a phenotype approximating to that seen in wg^l-8 mutants. The appearance of the denticle lawn in these embryos is noticeably different to that seen in maternal/zygotic arm^F1α/arm^F1α embryos zygotically mutant for wg^l-8. The denticle belts formed in sgg^M11; arm^F1α maternal/zygotic double mutant embryos are largely suppressed by Axn^S044230/Axn^S044230, leaving mainly naked cuticle without much sign of periodic character.

(Tolwinski et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

arm^F1a

(Mannava and Tolwinski, 2015, Kaplan et al., 2011, Kaplan et al., 2009, Tolwinski and Wieschaus, 2004)

arm^F1α

Name Synonyms

Secondary FlyBase IDs

References (5)

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