FlyBase Allele Report: Dmel\sgg[KK-MI.UAS.Tag:Myr(Src64B),Tag:HA]

General Information

Symbol

Dmel\sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0324780

Feature type

allele

Associated gene

Dmel\sgg

Associated Insertion(s)

Carried in Construct

P{UAS-myr-sgg.KK-MI.3xHA}

Key Links

Transgenic product class

missense_variant

(Mannava and Tolwinski, 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Mannava and Tolwinski, 2015)

Mutagen

in vitro construct

(Mannava and Tolwinski, 2015)

Progenitor genotype

Carried in construct

P{UAS-myr-sgg.KK-MI.3xHA}

Cytology

Description

UAS regulatory sequences drive expression of a kinase dead form of sgg (has K83M and K84I mutations in the ATP binding domain) that is tagged at the N-terminal end with the Tag:Myr(Src64B) membrane targeting sequence, and at the C-terminal end with 3 copies of Tag:HA.

(Mannava and Tolwinski, 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Embryos from mothers that express sgg^{KK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1} under the control of Scer\GAL4^arm.PU do not display any defects in the cuticle patterning and denticle belts' organization.

(Mannava and Tolwinski, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of segment polarity phenotype | embryonic stage phenotype of wg^l-8

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of segment polarity phenotype | embryonic stage | maternal effect phenotype of arm²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^da.PU is a non-enhancer of segment polarity phenotype | embryonic stage phenotype of arr²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of segment polarity phenotype | embryonic stage | maternal effect phenotype of dsh³

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of segment polarity phenotype | embryonic stage | maternal effect phenotype of arm^F1α

(Mannava and Tolwinski, 2015)

NOT Suppressor of

Statement

Reference

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of segment polarity phenotype | maternal effect | embryonic stage phenotype of arm²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of segment polarity phenotype | embryonic stage phenotype of wg^l-8

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of segment polarity phenotype | maternal effect | embryonic stage phenotype of arm^F1α

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^da.PU is a non-suppressor of segment polarity phenotype | embryonic stage phenotype of arr²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of segment polarity phenotype | maternal effect | embryonic stage phenotype of dsh³

(Mannava and Tolwinski, 2015)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of embryonic/larval cuticle | embryonic stage phenotype of wg^l-8

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of embryonic/larval cuticle | embryonic stage | maternal effect phenotype of arm^F1α

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of denticle field | embryonic stage | maternal effect phenotype of arm^F1α

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of embryonic/larval cuticle | embryonic stage | maternal effect phenotype of arm²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of denticle field | embryonic stage | maternal effect phenotype of arm²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of denticle field | embryonic stage phenotype of wg^l-8

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^da.PU is a non-enhancer of embryonic/larval cuticle | embryonic stage phenotype of arr²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^da.PU is a non-enhancer of denticle field | embryonic stage phenotype of arr²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of embryonic/larval cuticle | embryonic stage | maternal effect phenotype of dsh³

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-enhancer of denticle field | embryonic stage | maternal effect phenotype of dsh³

(Mannava and Tolwinski, 2015)

NOT Suppressor of

Statement

Reference

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of embryonic/larval cuticle | maternal effect | embryonic stage phenotype of arm²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of embryonic/larval cuticle | maternal effect | embryonic stage phenotype of dsh³

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of denticle field | maternal effect | embryonic stage phenotype of dsh³

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of embryonic/larval cuticle | embryonic stage phenotype of wg^l-8

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of denticle field | embryonic stage phenotype of wg^l-8

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of denticle field | maternal effect | embryonic stage phenotype of arm²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of embryonic/larval cuticle | maternal effect | embryonic stage phenotype of arm^F1α

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU is a non-suppressor of denticle field | maternal effect | embryonic stage phenotype of arm^F1α

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^da.PU is a non-suppressor of embryonic/larval cuticle | embryonic stage phenotype of arr²

(Mannava and Tolwinski, 2015)

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^da.PU is a non-suppressor of denticle field | embryonic stage phenotype of arr²

(Mannava and Tolwinski, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The segment polarity phenotype and the lack of naked cuticle between denticle belts, resulting in a lawn of denticles on the ventral epidermis, characteristic for maternally and zygotically mutant arm² or arm^F1α or arr² or dsh³ embryos as well as the zygotically mutant wg^l-8 embryos is not modified by Scer\GAL4^arm.PU-driven (or Scer\GAL4^da.PU-driven, in the arr² mutants) expression of sgg^{KK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1}.

(Mannava and Tolwinski, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU fails to rescue sgg^M11

(Mannava and Tolwinski, 2015)

Comments

The naked cuticle phenotype characteristic for both maternally and zygotically mutant sgg^M11 embryos cannot be rescued by expression of sgg^{KK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1} controlled by Scer\GAL4^arm.PU.

(Mannava and Tolwinski, 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sgg^{KK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1}

sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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