FlyBase Allele Report: Dmel\Egfr[GD1654]

General Information

Symbol

Dmel\Egfr^GD1654

Species

D. melanogaster

Name

FlyBase ID

FBal0206949

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{GD1654}

Also Known As

UAS-EGFR-RNAi, UAS-Egfr^RNAi, UAS-EgfrRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1654}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1654

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Corl et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates prostate cancer

modeled by Ras85D^V12.UAS

(Rambur et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

chemical sensitive, with Scer\GAL4^elav-C155

(Corl et al., 2009)

decreased size | adult stage, with Scer\GAL4^GMR.PU

(Bosso et al., 2023)

majority die during third instar larval stage, with Scer\GAL4^Act.PU

(Bosso et al., 2023)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PF

(Corl et al., 2009)

visible | adult stage, with Scer\GAL4^GMR.PU

(Bosso et al., 2023)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Corl et al., 2009)

eye, with Scer\GAL4^GMR.PU

(Bosso et al., 2023)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Males expressing Egfr^GD1654 under the control of Scer\GAL4^Abd-B-LDN show normal genitalia rotation.

(Lee et al., 2011)

Adults expressing Egfr^GD1654 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression of Egfr^GD1654 under the control of Scer\GAL4^elav-C155 results in flies with increased sensitivity to ethanol-induced sedation compared to control flies.

Expression of Egfr^GD1654 under the control of Scer\GAL4^GMR.PF results in a mild rough eye phenotype.

(Corl et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 30-40% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 10-20% or 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1654

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Egfr^GD1654, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by hppy^RB.UAS, Scer\GAL4^GMR.PF

(Corl et al., 2009)

Suppressed by

Statement

Reference

Egfr^GD1654, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by HP1b^GD10762, Scer\GAL4^GMR.PU

(Bosso et al., 2023)

Egfr^GD1654, Scer\GAL4^elav-C155 has chemical sensitive phenotype, suppressible by hppy^KG05537

(Corl et al., 2009)

Egfr^GD1654, Scer\GAL4^elav-C155 has chemical sensitive phenotype, suppressible by hppy^17-51

(Corl et al., 2009)

Suppressor of

Statement

Reference

Egfr^GD1654, Scer\GAL4^Act.PU is a suppressor | partially of increased mortality during development phenotype of HP1b^GD10762, Scer\GAL4^Act.PU

(Bosso et al., 2023)

Egfr^GD1654, Scer\GAL4^GMR.PU is a suppressor | partially of decreased size | adult stage phenotype of HP1b^GD10762, Scer\GAL4^GMR.PU

(Bosso et al., 2023)

Scer\GAL4^elav-C155/Egfr^GD1654 is a suppressor | partially of abnormal sleep | recessive phenotype of rho-5^KO1

(Zettl et al., 2011)

Scer\GAL4^NP5130/Egfr^GD1654 is a suppressor of increased cell number | somatic clone | cell autonomous phenotype of hpo^BF33

(Ren et al., 2010)

Scer\GAL4^NP5130/Egfr^GD1654 is a suppressor of increased occurrence of cell division | somatic clone | adult stage phenotype of hpo^BF33

(Ren et al., 2010)

NOT Suppressor of

Statement

Reference

Egfr^GD1654, Scer\GAL4^A9 is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Egfr^GD1654, Scer\GAL4^A9 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Scer\GAL4^NP5130/Egfr^GD1654 is a non-suppressor of increased cell number | somatic clone | cell non-autonomous phenotype of hpo^BF33

(Ren et al., 2010)

Scer\GAL4^NP5130/Egfr^GD1654 is a non-suppressor of increased occurrence of cell division | somatic clone | cell non-autonomous | adult stage phenotype of hpo^BF33

(Ren et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr^GD1654, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by hppy^RB.UAS, Scer\GAL4^GMR.PF

(Corl et al., 2009)

Suppressed by

Statement

Reference

Egfr^GD1654, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by HP1b^GD10762, Scer\GAL4^GMR.PU

(Bosso et al., 2023)

Suppressor of

Statement

Reference

Scer\GAL4^NP5130/Egfr^GD1654 is a suppressor of intestinal stem cell | somatic clone phenotype of hpo^BF33

(Ren et al., 2010)

Scer\GAL4^btl.PS/Egfr^GD1654 is a suppressor of larval tracheal system phenotype of Ptp10D¹, Ptp4E¹

(Jeon and Zinn, 2009)

NOT Suppressor of

Statement

Reference

Egfr^GD1654, Scer\GAL4^A9 is a non-suppressor of wing vein phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Egfr^GD1654/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

Other

Statement

Reference

Egfr^GD1654, Scer\GAL4^Act.PU, aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

The thickened wing vein phenotype of adults expressing anchor^GD3613 under the control of Scer\GAL4^A9 is not suppressed by addition of Egfr^GD1654.

(Wang et al., 2018)

Border cell clusters in females co-expressing Egfr^GD1654 and Pvr^GD2571 under the control of Scer\GAL4^slbo.2.6 show a significantly reduced net cluster movement compared to wild type. There is a reduction in the number of cellular extensions from the front of the border cell cluster compared to wild type.

(Poukkula et al., 2011)

Expression of Egfr^GD1654 under the control of Scer\GAL4^elav-C155 significantly suppresses the sleep defects seen in homozygous rho-5^KO1 mutants.

(Zettl et al., 2011)

Compared with hpo^BF33 mutant MARCM clones, hpo^BF33 mutant clones expressing Egfr^GD1654 exhibit reduced clone size. Egfr^GD1654 expression suppresses the elevated levels of mitosis seen in hpo^BF33 MARCM clones but not outside the expression domain of the MARCM clones.

(Ren et al., 2010)

The mild rough eye phenotype caused by expression of Egfr^GD1654 under the control of Scer\GAL4^GMR.PF is potently enhanced by co-expression of hppy^RB.Scer\UAS.

The increased sensitivity to ethanol-induced sedation caused by expression of Egfr^GD1654 under the control of Scer\GAL4^elav-C155 is completely suppressed by either hppy^KG05537 or hppy^17-51.

(Corl et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v43267