FlyBase Allele Report: Dmel\hpo[BF33]

General Information

Symbol

Dmel\hpo^BF33

Species

D. melanogaster

Name

FlyBase ID

FBal0151854

Feature type

allele

Associated gene

Dmel\hpo

Associated Insertion(s)

Carried in Construct

Also Known As

dMST^BF33

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Jia et al., 2003)

Mutagen

ethyl methanesulfonate

(Jia et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?174term.

(Jia et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:19,496,227..19,496,227 [-]

Nucleotide change:

T19496227R

Amino acid change:

Y174term | hpo-PA; Y174term | hpo-PB

Reported amino acid change:

?174term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Jia et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Napoletano et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | somatic clone

(Jia et al., 2003)

abnormal neuroanatomy | adult stage | progressive | somatic clone

(Napoletano et al., 2011)

decreased cell death | somatic clone

(Jia et al., 2003)

increased cell number | cell non-autonomous | somatic clone

(Ren et al., 2010)

increased cell number | somatic clone

(Jia et al., 2003)

increased occurrence of cell division | adult stage | cell non-autonomous | somatic clone

(Ren et al., 2010)

increased occurrence of cell division | adult stage | somatic clone

(Ren et al., 2010)

lethal - all die before end of larval stage | recessive

(Jia et al., 2003)

neoplasia | somatic clone

(Jia et al., 2003)

some die during larval stage | recessive

(Jia et al., 2003)

visible | adult stage | somatic clone

(Hu et al., 2016)

visible | somatic clone

(Guo et al., 2013, Jin et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult eyes containing hpo^BF33 homozygous somatic clones (induced by the eyFLP method) display overgrowth and folding phenotype.

(Hu et al., 2016)

Homozygous clones in the eye disc result in overgrowth and adults have enlarged and folded eyes.

(Guo et al., 2013)

hpo^BF33 mutant eye disc clones exhibit tumorous overgrowth.

(Huang et al., 2013)

Homozygous clones in the eye result in enlarged and folded eyes.

(Jin et al., 2012)

Homozygous clones in the eye show progressive degeneration of photoreceptor cells.

(Napoletano et al., 2011)

hpo^BF33 mutant MARCM clones contain 5-7 cells per clone, compared to 2-3 cells in wild-type clones. These clones contain differentiated absorptive enterocytes and secretory enteroendocrine cells indicating that intestine stem cell differentiation continues as in wild-type. Phospho-His3 staining reveals an increase in the number of mitotic cells within the mutant clones. There is also an increase in the number of mitotic cells outside of the hpo^BF33 mutant clones, indicating non-cell-autonomous proliferation.

(Ren et al., 2010)

Adults containing homozygous clones represent 36.4% of the population recovered after clones are induced using the eyFLP method (expected fraction of adults containing mutant clones is 50% if there is no effect of the mutant clones on viability).

(Genevet et al., 2009)

hpo^BF33 mosaic eyes are significantly larger and often protrude out in folds. Tumourous outgrowths are also seen when clones are induced in other places, including the thorax wing and haltere. When mutant clones are made in the wing disc the size of the clones are significantly larger with more cells than the wild-type twin spots. The cell size is unaffected. More cells posterior to the second mitotic wave are seen to be in M phase than in wild-type.

(Jia et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hpo^BF33 has visible | adult stage | somatic clone phenotype, enhanceable by Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU

(Hu et al., 2016)

NOT Enhanced by

Statement

Reference

hpo^BF33 has visible | adult stage | somatic clone phenotype, non-enhanceable by Scer\GAL4^Ubi.PU/Ack^{KD.UAS.Tag:V5}

(Hu et al., 2016)

Suppressed by

Statement

Reference

hpo^BF33 has visible | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/Tgi^UAS.Tag:FLAG

(Guo et al., 2013)

hpo^BF33 has increased occurrence of cell division | somatic clone | adult stage phenotype, suppressible by dome^GD14494/Scer\GAL4^NP5130

(Ren et al., 2010)

hpo^BF33 has increased occurrence of cell division | somatic clone | cell non-autonomous | adult stage phenotype, suppressible by dome^GD14494/Scer\GAL4^NP5130

(Ren et al., 2010)

hpo^BF33 has increased occurrence of cell division | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^NP5130/Egfr^GD1654

(Ren et al., 2010)

hpo^BF33 has increased cell number | somatic clone | cell autonomous phenotype, suppressible by dome^GD14494/Scer\GAL4^NP5130

(Ren et al., 2010)

hpo^BF33 has increased cell number | somatic clone | cell autonomous phenotype, suppressible by Scer\GAL4^NP5130/Egfr^GD1654

(Ren et al., 2010)

NOT suppressed by

Statement

Reference

hpo^BF33 has increased cell number | somatic clone | cell non-autonomous phenotype, non-suppressible by dome^GD14494/Scer\GAL4^NP5130

(Ren et al., 2010)

hpo^BF33 has increased cell number | somatic clone | cell non-autonomous phenotype, non-suppressible by Scer\GAL4^NP5130/Egfr^GD1654

(Ren et al., 2010)

hpo^BF33 has increased occurrence of cell division | somatic clone | cell non-autonomous | adult stage phenotype, non-suppressible by Scer\GAL4^NP5130/Egfr^GD1654

(Ren et al., 2010)

Suppressor of

Statement

Reference

hpo^BF33 is a suppressor of increased cell death | somatic clone phenotype of hid^GMR.PG

(Jia et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

hpo^BF33 has eye | adult stage | somatic clone phenotype, enhanceable by Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU

(Hu et al., 2016)

NOT Enhanced by

Statement

Reference

hpo^BF33 has eye | adult stage | somatic clone phenotype, non-enhanceable by Scer\GAL4^Ubi.PU/Ack^{KD.UAS.Tag:V5}

(Hu et al., 2016)

Suppressed by

Statement

Reference

hpo^BF33 has eye | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/Tgi^UAS.Tag:FLAG

(Guo et al., 2013)

hpo^BF33 has intestinal stem cell | somatic clone phenotype, suppressible by dome^GD14494/Scer\GAL4^NP5130

(Ren et al., 2010)

hpo^BF33 has intestinal stem cell | somatic clone phenotype, suppressible by Scer\GAL4^NP5130/Egfr^GD1654

(Ren et al., 2010)

NOT suppressed by

Statement

Reference

hpo^BF33 has eye disc | somatic clone phenotype, non-suppressible by par-1^HMS00405/Scer\GAL4^unspecified

(Huang et al., 2013)

Enhancer of

Statement

Reference

hpo^BF33 is an enhancer of eye disc phenotype of hid^GMR.PG

(Jia et al., 2003)

Other

Statement

Reference

Scer\GAL4^Tub.PU, brm^GD4507, hpo^BF33 has midgut | somatic clone phenotype

(Jin et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ack^{Scer\UAS.T:SV5\V5} (but not Ack^{KD.Scer\UAS.T:SV5\V5}) under the control of Scer\GAL4^Ubi.PU in the hpo^BF33 homozygous somatic clones induced in the eye tissue significantly enhances the overgrowth phenotype observed in the adult eye containing hpo^BF33 clones.

(Hu et al., 2016)

Expression of Tgi^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^tub.PU in hpo^BF33 clones in the eye disc results in adults with nearly normal eyes.

(Guo et al., 2013)

Expression of par-1^HMS00405 under the control of Scer\GAL4^unspecified does not suppress the tumorous overgrowth seen in hpo^BF33 mutant eye disc clones.

(Huang et al., 2013)

Expression of brm^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^tub.PU does not affect the growth of hpo^BF33 mutant midgut clones.

Expression of brm^GD4507 under the control of Scer\GAL4^tub.PU reduces the growth of hpo^BF33 mutant midgut clones.

(Jin et al., 2013)

Expressing yki^{dsRNA.N.Scer\UAS} in hpo^BF33 mutant MARCM clones (using Scer\GAL4^esg-NP5130) reduces the clone size.

Compared with hpo^BF33 mutant MARCM clones, hpo^BF33 mutant clones expressing dome^GD14494 or Egfr^GD1654 exhibit reduced clone size. dome^GD14494 or Egfr^GD1654 expression suppresses the elevated levels of mitosis seen in hpo^BF33 MARCM clones but not outside the expression domain of the MARCM clones.

(Ren et al., 2010)

When hpo^BF33 clones are made in a W^GMR.PG background the resultant eyes are larger than that seen in W^GMR.PG animals alone.

(Jia et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

hpo^BF33 is rescued by hpo^{UAS.WT.Tag:FLAG}/Scer\GAL4^Tub.PU

(Jin et al., 2012)

hpo^BF33 is rescued by hpo^{T195E.UAS.Tag:FLAG}/Scer\GAL4^Tub.PU

(Jin et al., 2012)

Partially rescued by

hpo^BF33 is partially rescued by Scer\GAL4^Tub.PU/hpo^{M242E.UAS.Tag:FLAG}

(Jin et al., 2012)

Comments

Expression of hpo^{Scer\UAS.WT.T:Zzzz\FLAG} under the control of Scer\GAL4^tub.PU almost completely rescues the overgrowth phenotype caused by hpo^BF33 clones in the eye.

Expression of hpo^{T195E.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^tub.PU rescues the overgrowth phenotype caused by hpo^BF33 clones in the eye.

Expression of hpo^{M242E.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^tub.PU partially rescues the overgrowth phenotype caused by hpo^BF33 clones in the eye.

(Jin et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600507

w^*; P{neoFRT}42D hpo^BF33/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dMST^BF33

(Zhang et al., 2008, Jia et al., 2003)

hpo^BF33

(Tokamov et al., 2021, Hu et al., 2016, Wang et al., 2016, Dong et al., 2015, Sarikaya and Extavour, 2015, Hu et al., 2014, Guo et al., 2013, Huang et al., 2013, Jin et al., 2012, Napoletano et al., 2011, Ho et al., 2010, Ren et al., 2010, Genevet et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (16)

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