FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\l(2)glGD4047
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General Information
Symbol
Dmel\l(2)glGD4047
Species
D. melanogaster
Name
FlyBase ID
FBal0209863
Feature type
allele
Associated gene
Dmel\l(2)gl
Associated Insertion(s)
Carried in Construct
P{GD4047}
Also Known As
UAS-lgl-RNAi, UAS-lglRNAi, UAS-lgl-IR
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD4047}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD4047
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  cancer
      is exacerbated by Stat92EΔNΔC.UASp.Tag:HA
      (Tamori et al., 2016)
      is ameliorated by Stat92EKK100519
      (Tamori et al., 2016)
      model of  carcinoma
      is exacerbated by Nint.U.UAS
      (Jevitt et al., 2021)
      is exacerbated by NCA.UAS.cUa
      (Jevitt et al., 2021)
      is ameliorated by NJF01637
      (Chatterjee et al., 2023)
      is exacerbated by NNICD.UASp
      (Chatterjee et al., 2023)
      is ameliorated by Stat92EKK100519
      (Chatterjee et al., 2023)
      is exacerbated by esgEY10592
      (Chatterjee et al., 2023)
      is exacerbated by upd1UAS.cZa
      (Chatterjee et al., 2023)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of l(2)glGD4047 under the control of Scer\GAL4Bx-MS1096 or Scer\GAL4Hml.Δ does not lead to a significant difference in survival as compared to controls when larvae are infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens.

      (Arefin et al., 2017)

      The expression of l(2)glGD4047 under the control of Scer\GAL4ptc-559.1 leads to an increase in apoptosis around the anterior-posterior compartment boundary of the third instar larval wing disc, as compared to controls.

      (Ren et al., 2017)

      Somatic clones expressing l(2)glGD4047 under the control of Scer\GAL4Act.PU in wandering third instar larval wing discs do not present obvious size differences, as compared to control clones.

      (Casas-Tintó et al., 2016)

      The expression of l(2)glGD4047 under the control of Scer\GAL4ptc-559.1 (together with Dicer-2, for efficient RNAi) leads to third instar larval wing discs displaying dysplastic overgrowths in the dorsal hinge region and displaying apoptosis at the boundary with non-expressing cells along the ptc-positive domain at the anterior-posterior compartment boundary, as compared to controls. Expression under the control of Scer\GAL4sd-SG29.1 also leads to dysplastic overgrowths in the wing disc hinge region, as compared to controls.

      The clonal expression of l(2)glGD4047 under the control of Scer\GAL4Act5C.PI (together with Dicer-2, for efficient RNAi) within mosaic third instar larval wing discs leads to dysplastic overgrowths only in the hinge region and leads to apoptosis (Dcp1 staining) and basal delamination/extrusion of some l(2)glGD4047-expressing cells in both the wing pouch and hinge regions (particularly at the boundary with non-expressing cells), as compared to controls. The overgrowths are particularly prevalent at the dorsal hinge region, mostly at medial fold, and always occur at the apical side of the epithelial layer. Despite of the basal delamination, some of the expressing cells in the hinge region remain within the epithelial layer.

      (Tamori et al., 2016)

      Expression of l(2)glGD4047 under the control of Scer\GAL4en.PU does not induce wing disc overgrowth at 25[o]C. At 29[o]C, morphological alterations of the P-compartment are evident, although some fragmentation is also seen in controls.

      (Grifoni et al., 2015)

      Expression of l(2)glGD4047 using a particular insertion line - VDRC #51249 - under the control of Scer\GAL4e22c results in abnormal large epidermal syncytia being formed in about 40% of the larvae, however when a different insertion (VDRC #51247) of the same is used, no such phenotype is observed.

      (Wang et al., 2015)

      Adults expressing l(2)glGD4047 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      l(2)glGD4047/Scer\GAL4dome-PG5 is a non-enhancer of melanotic mass phenotype phenotype of hopTum

      (Bina et al., 2010)
      NOT Suppressor of
      Statement
      Reference

      l(2)glGD4047/Scer\GAL4dome-PG5 is a non-suppressor of melanotic mass phenotype phenotype of hopTum

      (Bina et al., 2010)
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      l(2)glGD4047/Scer\GAL4dome-PG5 is a non-enhancer of melanotic mass phenotype of hopTum

      (Bina et al., 2010)
      NOT Suppressor of
      Statement
      Reference

      l(2)glGD4047/Scer\GAL4dome-PG5 is a non-suppressor of melanotic mass phenotype of hopTum

      (Bina et al., 2010)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased apoptosis around the anterior-posterior compartment boundary of the third instar larval wing disc resulting from the expression of l(2)glGD4047 under the control of Scer\GAL4ptc-559.1 is strongly suppressed by the co-expression of GlyatTH00482.

      (Ren et al., 2017)

      Somatic clones co-expressing wupAf06492 and l(2)glGD4047 under the control of Scer\GAL4Act.PU in wandering third instar larval wing discs present a significant increase in the relative clonal area, as compared to control clones; animals bearing clones co-expressing l(2)glGD4047 and wupAf06492 do not reach adulthood.

      Somatic clones co-expressing l(2)glGD4047, Ras85DV12.cUa.Scer\UAS and wupAf06492 under the control of Scer\GAL4Act.PU in wandering third instar larvae wing discs exhibit a significant increase in relative clonal area as compared to clones only co-expressing l(2)glGD4047 and Ras85DV12.cUa.Scer\UAS or only expressing wupAf06492; animals bearing clones co-expressing l(2)glGD4047, Ras85DV12.cUa.Scer\UAS and wupAf06492 do not reach adulthood.

      The induction of somatic clones co-expressing l(2)glGD4047 and Ras85DV12.cUa.Scer\UAS under the control of Scer\GAL4Act.PU during second instar larval stage results in the accumulation of clonal cells in the wandering third instar larval fat body, which is enhanced by the additional co-expression of wupAJF02172; the induction of these clones also results in lethality before adulthood, which is partially rescued by the additional co-expression of wupAJF02172, although the surviving adults display a shorter life-span as compared to wild-type controls.

      In wandering third instar larvae, somatic clones co-expressing l(2)glGD4047 and Ras85DV12.cUa.Scer\UAS under the control of Scer\GAL4Act.PU in both eye discs and wing discs exhibit a significant increase in the relative clonal area and hyperplasia as compared to control clones; wing disc clone cells localize both basally and apically in the disc. All of these phenotypes are suppressed by the additional co-expression of wupAJF02172, even leading to a significant decrease in the wing disc clonal area as compared to control clones.

      (Casas-Tintó et al., 2016)

      The wing disc hinge overgrowths induced by the clonal expression of l(2)glGD4047 under the control of Scer\GAL4Act5C.PI (together with Dicer-2, for efficient RNAi) is enhanced by the co-expression of Stat92EΔNΔC.UASp.Tag:HA, leading to large tumor masses, while being suppressed the co-expression of Stat92EKK100519; the Stat92EΔNΔC.UASp.Tag:HA co-expression still does not lead to overgrowths in the wing pouch area and does not affect the basal delamination/extrusion observed upon scribKK101128 single expression.

      The clonal co-expression of l(2)glGD4047 and RhoGEF2HMS01118 under the control of Scer\GAL4Act5C.PI (together with Dicer-2, for efficient RNAi) in third instar larval wing discs leads to a significant proportion of apical delamination, which does not occur in l(2)glGD4047 single expression clones.

      (Tamori et al., 2016)

      Larvae expressing l(2)glGD4047 under the control of Scer\GAL4en.PU in a minute background (RpL27A1) live for about 11 days after egg laying and die with no signs of differentiation. The cells of the P-compartment of the wing disc appear highly disorganised due to loss of apical-basal polarity and increased hypoxia is seen compared to controls. At the extreme end of the larval stage these tumor cells are highly migratory, migrating towards the tracheal tubes and contacting their filopodia. The tumor cells also form new tracheal branches and can migrate along tracheal vessels and fuse with them. Tumor cells are seen to migrate from one disc to another, for example from the haltere disc, along a tracheal scaffold, to the wing disc.

      Expression of bskDN.Scer\UAS suppresses the tumor growth seen in the wing disc when l(2)glGD4047 is expressed under the control of Scer\GAL4en.PU in a minute background (RpL27A1). Individuals differentiate into adult pharates and a small proportion of the pupae eclose into adults with no evident phenotypic alterations.

      (Grifoni et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      LglRNAi#1
      (Wang et al., 2015)
      LglRNAi#2
      (Wang et al., 2015)
      l(2)glGD4047
      Name Synonyms
      Secondary FlyBase IDs
        References (20)