Expression of both Nint.U.Scer\UAS and tjNIG.10034R under the control of Scer\GAL4tj-NP1624 leads to severe ovary defects. Most ovaries are extremely small, not connected to an oviduct and often attached to the gut, and consist largely of terminal filaments and a few germline cells embedded in fat body. The epithelial sheaths are missing and terminal filaments are located side-by-side. In the most severe cases, these ovaries consist only of terminal filaments. Terminal filaments in these flies are on average longer than those in flies expressing only Nint.U.Scer\UAS, but slightly shorter than those in flies expressing only tjNIG.10034R.
Somatic clones co-expressing wupAf06492 and Nint.U.Scer\UAS under the control of Scer\GAL4Act.PU in wandering third instar larval wing discs present a significant increase in the relative clonal area, as compared to clones only expressing Nint.U.Scer\UAS but not to clones only expressing wupAf06492; animals bearing clones co-expressing wupAf06492 and Nint.U.Scer\UAS occasionally reach adulthood and present visible outgrowths.
Expression of Nint.U.Scer\UAS under the control of Scer\GAL4dpp.blk1 in a nctST1/nctST3 mutant background results in larger third instar eye discs compared to wild-type. The missing neuronal clusters observed in Nint.U.Scer\UAS; Scer\GAL4dpp.blk1 eye discs are restored in a nctST1/nctST3 mutant background. However, R8 cells are not as regularly arrayed as in wild-type or Nint.U.Scer\UAS; Scer\GAL4dpp.blk1 eye discs. An increase R8 cell number is also observed, including more than one R8 cell per cluster.
The co-expression of Nint.U.Scer\UAS and Hsap\UBE3AScer\UAS.cRa with HPV18\E6Scer\UAS.T:Hsap\MYC, but not with HPV18\E6V158A.Scer\UAS.T:Hsap\MYC, under the control of Scer\GAL4GMR.PU, leads to the migration of a considerable proportion of the expressing eye cells into distant pupal tissues (e.g. abdomen), as compared to controls.