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General Information
Symbol
Dmel\Nint.U.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0182588
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Nintra, UAS-NICD, uas-Nicd, NICD, UAS-NotchΔECD
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of activated N.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Nint.U.Scer\UAS under the control of Scer\GAL4tj-NP1624 leads to ovaries with a large increase in the number of cap cells, as compared to controls, and some germaria with a cluster of undifferentiated germ cells that resemble germline stem cells, while other germaria are devoid of germ cells altogether.

Expression of Nint.U.Scer\UAS under the control of Scer\GAL4elav-C155 in the larval optic lobe (in combination with a Gal80[ts] transgene to restrict expression to the larval stage) leads to ectopic neuroblasts among medulla neurons.

Larval optic lobe post-mitotic neuron clones expressing Nint.U.Scer\UAS under the control of Scer\GAL4Act.PU include ectopic neuroblasts.

Somatic clones expressing Nint.U.Scer\UAS under the control of Scer\GAL4Act.PU in wandering third instar wing discs present a significant increase in relative clonal area as compared to controls.

The induction of somatic clones expressing Nint.U.Scer\UAS under the control of Scer\GAL4GMR.PU leads to a significant decrease in the eclosion rate, as compared to controls.

Constitutive expression of Nint.U.Scer\UAS under the control of Scer\GAL4GMR.PU does not lead to any obvious cell migration defects in the pupal eye tissue, as compared to controls.

Expression of Nint.U.Scer\UAS under the control of Scer\GAL4Antp-21 in third instar larvae results in a significant increase in crystal cell number in lymph gland anterior lobes, and these develop in abnormal clusters, in contrast to controls.

Expression of Nint.U.Scer\UAS under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C in third instar lymph gland clones induces crystal cell fate both in the primary lobe, but occasionally also in the posterior lobe, where crystal cells are not normally found.

Clones expressing Nint.U.Scer\UAS under the control of Scer\GAL4Act5C.PU show an increase in the number of neuroblasts per clone compared to wild type.

The gl-positive corpora cardiaca precursor cells are missing in stage 12 embryos expressing Nint.U.Scer\UAS under the control of either Scer\GAL4twi.2PE or Scer\GAL4Mef2.PR.

The number of gl-positive corpora cardiaca precursor cells present at stage 12 is not altered by expression of Nint.U.Scer\UAS under the control of either Scer\GAL4gl.5.2 or Scer\GAL4gt.1.CRM.

Transient expression of Nint.U.Scer\UAS under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B for seven days in young flies shortens lifespan significantly compared to controls. Longevity is not significantly affected when these flies are kept at permissive temperature throughout life.

Expression of Nint.U.Scer\UAS under the control of Scer\GAL4dpp.3KK eliminates the wing veins.

Expression of Nint.U.Scer\UAS in the eye disc under the control of Scer\GAL4dpp.blk1 results in larger third instar larval eye discs compared to wild-type. Proper R8 neuron specification is observed. Neuronal clusters are highly disorganised or absent in the posterior region of the disc.

Expression of Nint.U.Scer\UAS under the control of Scer\GAL4GMR.PF results in disruption of cluster assembly and neuronal differentiation in late third instar larval eye discs. Nearly normal selection of R8 neurons occurs, but aberrant formation of more posterior clusters, completely lacking R8 cells and with reduced numbers of photoreceptors.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT Enhancer of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of both Nint.U.Scer\UAS and tjNIG.10034R under the control of Scer\GAL4tj-NP1624 leads to severe ovary defects. Most ovaries are extremely small, not connected to an oviduct and often attached to the gut, and consist largely of terminal filaments and a few germline cells embedded in fat body. The epithelial sheaths are missing and terminal filaments are located side-by-side. In the most severe cases, these ovaries consist only of terminal filaments. Terminal filaments in these flies are on average longer than those in flies expressing only Nint.U.Scer\UAS, but slightly shorter than those in flies expressing only tjNIG.10034R.

Somatic clones co-expressing wupAf06492 and Nint.U.Scer\UAS under the control of Scer\GAL4Act.PU in wandering third instar larval wing discs present a significant increase in the relative clonal area, as compared to clones only expressing Nint.U.Scer\UAS but not to clones only expressing wupAf06492; animals bearing clones co-expressing wupAf06492 and Nint.U.Scer\UAS occasionally reach adulthood and present visible outgrowths.

The co-expression of wupAJF02172 suppresses the decreased eclosion rate of individuals bearing somatic clones expressing Nint.U.Scer\UAS under the control of Scer\GAL4GMR.PU.

Drp12 suppresses the increase in the number of neuroblasts per clone seen in clones expressing Nint.U.Scer\UAS under the control of Scer\GAL4Act5C.PU.

Expression of Nint.U.Scer\UAS under the control of Scer\GAL4dpp.blk1 in a nctST1/nctST3 mutant background results in larger third instar eye discs compared to wild-type. The missing neuronal clusters observed in Nint.U.Scer\UAS; Scer\GAL4dpp.blk1 eye discs are restored in a nctST1/nctST3 mutant background. However, R8 cells are not as regularly arrayed as in wild-type or Nint.U.Scer\UAS; Scer\GAL4dpp.blk1 eye discs. An increase R8 cell number is also observed, including more than one R8 cell per cluster.

Xenogenetic Interactions
Statement
Reference

The co-expression of Nint.U.Scer\UAS and Hsap\UBE3AScer\UAS.cRa with HPV18\E6Scer\UAS.T:Hsap\MYC, but not with HPV18\E6V158A.Scer\UAS.T:Hsap\MYC, under the control of Scer\GAL4GMR.PU, leads to the migration of a considerable proportion of the expressing eye cells into distant pupal tissues (e.g. abdomen), as compared to controls.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Nint.U.Scer\UAS
Nint.U.UAS
Name Synonyms
Secondary FlyBase IDs
    References (43)