FlyBase Allele Report: Dmel\par-6[Δ226]

General Information

Symbol

Dmel\par-6^Δ226

Species

D. melanogaster

Name

FlyBase ID

FBal0123759

Feature type

allele

Associated gene

Dmel\par-6

Associated Insertion(s)

Carried in Construct

Also Known As

par6^Δ226, par-6^D226

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Petronczki and Knoblich, 2001)

Progenitor genotype

P{EP}CG8188^EP1464

(Petronczki and Knoblich, 2001)

Caused by aberration

Df(1)par-6^Δ226

Cytology

Description

The promoter, start codon, and the first 121 amino acids of the protein is deleted.

(Huynh et al., 2001)

Imprecise excision of the P{EP}EP1464 element resulting in a deletion removing CG8188 and the first 121 residues of par-6.

(Petronczki and Knoblich, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous tracheal terminal cells show a reduction in class II branches compared to wild type, have very little gas-filled lumen and show an abnormal branch tip morphology.

Hemizygous embryos have a large hole in the cuticle.

(Jones and Metzstein, 2011)

Zygotic mutants for par-6^Δ226 complete embryogenesis due to maternally inherited gene product.

(David et al., 2010)

Somatic clones homozygous for par-6^Δ226 in the ovarian follicle cells cause discontinuities and multilayering of the epithelium. When the majority of border cells are part of a clone, the border cell cluster fails to migrate. Clusters with a minority of mutant cells migrate normally. The morphology of mutant clusters is frequently abnormal sometimes with cells trailing behind the migrating cluster.

Border cell migration is incomplete in 13% of stage 10 egg chambers from (par-6^{dsRNA.Sym.Scer\UAS})X2; Scer\GAL4^slbo.2.6 flies. This increases to 34% in flies also heterozygous for par-6^Δ226.

(Pinheiro and Montell, 2004)

heterozygotes have a decreased number of synaptic boutons at neuromuscular junctions.

(Ruiz-Canada et al., 2004)

Somatic clones of par-6^Δ226 in the pupal retina are very small - consisting of only a few cells, suggesting a role in proliferation and/or survival of retinal cells.

(Nam and Choi, 2003)

When mutant germ-line clones are made, the majority of resulting egg chambers appear small, oval shaped and contain 16 polyploid nurse cells and no oocyte. Furthermore centrosomes accumulate in one cell at the posterior of the cyst, although with a slight delay compared to wild-type. They remain at the anterior of the cell and fail to translocate to the posterior pole. Some eggs chambers escape the early arrest and go on to produce normal eggs. When females are scored 2 days after eclosion, half of the egg chambers form a normal oocyte. and a quarter still do after 10 days.

(Huynh et al., 2001)

Homozygous Df(1)par-6^Δ226 late stage embryos have large holes in the cuticle at random positions. Only a small number of eggs are recovered from homozygous Df(1)par-6^Δ226 female germline clones. Early development is normal in these embryos, but epithelial cells lose their regular arrangement and become rounded, and often undergo apoptosis after germband retraction. Metaphase plates are often misorientated in neuroblasts (25% are misorientated by more than 60^o relative to the horizontal plane and 37% are misorientated by between 30^o and 60^o).

(Petronczki and Knoblich, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

par-6^Δ226 has abnormal neuroanatomy phenotype, non-enhanceable by aPKC^Exc55

(Ruiz-Canada et al., 2004)

NOT suppressed by

Statement

Reference

par-6^Δ226 has abnormal neuroanatomy phenotype, non-suppressible by aPKC^Exc55

(Ruiz-Canada et al., 2004)

Enhancer of

Statement

Reference

par-6[+]/par-6^Δ226 is an enhancer of visible | dominant phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

NOT Enhancer of

Statement

Reference

par-6[+]/par-6^Δ226 is a non-enhancer of abnormal neuroanatomy phenotype of aPKC^Exc55

(Ruiz-Canada et al., 2004)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

par-6^Δ226, zip¹ has embryonic/first instar larval cuticle phenotype, non-enhanceable by aPKC^k06403

(David et al., 2010)

par-6^Δ226 has bouton phenotype, non-enhanceable by aPKC^Exc55

(Ruiz-Canada et al., 2004)

NOT suppressed by

Statement

Reference

par-6^Δ226 has bouton phenotype, non-suppressible by aPKC^Exc55

(Ruiz-Canada et al., 2004)

Enhancer of

Statement

Reference

par-6^Δ226 is an enhancer of embryonic/first instar larval cuticle phenotype of zip¹

(David et al., 2010)

par-6[+]/par-6^Δ226 is an enhancer of eye phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

NOT Enhancer of

Statement

Reference

par-6^Δ226 is a non-enhancer of embryonic/first instar larval cuticle phenotype of aPKC^k06403, zip¹

(David et al., 2010)

par-6[+]/par-6^Δ226 is a non-enhancer of ommatidium phenotype of Vang^stbm-153

(Courbard et al., 2009)

NOT Suppressor of

Statement

Reference

par-6[+]/par-6^Δ226 is a non-suppressor of ommatidium phenotype of Vang^stbm-153

(Courbard et al., 2009)

par-6[+]/par-6^Δ226 is a non-suppressor of bouton phenotype of aPKC^Exc55

(Ruiz-Canada et al., 2004)

Other

Statement

Reference

par-6^Δ226, zip² has embryonic/first instar larval cuticle phenotype

(David et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

par-6^Δ226, zip¹ double mutants display a prominent dorsal hoe merged with a large head hole indicating enhancement of the zip¹ embryonic phenotype. par-6^Δ226, zip¹, aPKC^k06403 triple mutants show similar enhancement.

par-6^Δ226, zip² double mutant embryos display merged dorsal and head holes.

(David et al., 2010)

Heterozygosity for par-6^Δ226 has no effect on the frequency of ommatidia that show planar cell polarity defects in Vang^stbm-153 homozygotes.

(Courbard et al., 2009)

Expression of Rho1^{1.dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI does not affect the adherens junctions between par-6^Δ226 mutant cells.

(Warner and Longmore, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

par-6^29VV

(Jones and Metzstein, 2011)

Rescued by

par-6^Δ226 is rescued by Scer\GAL4^wor.PA/par-6^UAS.Tag:HA

(Atwood et al., 2007)

Not rescued by

par-6^Δ226 is not rescued by Scer\GAL4^wor.PA/par-6^{ISAA.UAS.Tag:HA}

(Atwood et al., 2007)

Comments

Expression of par-6^{Scer\UAS.T:Ivir\HA} under the control of Scer\GAL4^wor.PA in par-6^Δ226 mutant animals can rescue neuroblast polarity defects.

Expression of par-6^{ISAA.Scer\UAS.T:Ivir\HA} under the control of Scer\GAL4^wor.PA in par-6^Δ226 mutant animals fails to rescue neuroblast polarity defects.

(Atwood et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

DmPar-6^Δ226

(Ruiz-Canada et al., 2004)

par-6²²⁶

(Wang and Riechmann, 2007)

par-6^D226

(Carvalho et al., 2015, Morais-de-Sá et al., 2010)

par-6^Delta226

(Zarnescu et al., 2005)

par-6^Δ226

(Wu et al., 2023, Dong et al., 2020, Khoury and Bilder, 2020, Dong et al., 2015, Leibfried et al., 2013, Weber et al., 2012, Jones and Metzstein, 2011, David et al., 2010, Kim et al., 2009, Ogawa et al., 2009, Warner and Longmore, 2009, Wirtz-Peitz et al., 2008, Wirtz-Peitz et al., 2008, Hutterer et al., 2004)

par6²²⁶

(Besson et al., 2015)

par6^D226

(Spindler and Hartenstein, 2011)

par6^Δ226

(Liu et al., 2020, Nunes de Almeida et al., 2019, Chen et al., 2018, Jiang et al., 2014, Spindler and Hartenstein, 2011, Courbard et al., 2009, Denef et al., 2008, Atwood et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (33)

Report Sections

Open Close