FlyBase Allele Report: Dmel\Egfr[1.A887T.UAS]

General Information

Symbol

Dmel\Egfr^1.A887T.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0095743

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{Egfr.1.A887T.UAS}

Also Known As

UAS-EGFR^A887T, UAS-Egfr^Elp type I

Key Links

Transgenic product class

missense_variant

(Lesokhin et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Lesokhin et al., 1999)

Mutagen

in vitro construct

(Lesokhin et al., 1999)

Progenitor genotype

Carried in construct

P{Egfr.1.A887T.UAS}

Cytology

Description

Expression of mutant Type 1 Egfr coding region (carries the amino acid replacement A887T) is governed by UASt regulatory sequences.

(Lesokhin et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | somatic clone, with Scer\GAL4^NP5130

(Ren et al., 2010)

increased occurrence of cell division | adult stage | somatic clone, with Scer\GAL4^NP5130

(Ren et al., 2010)

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Maybeck and Röper, 2009)

eye, with Scer\GAL4^h-H10

(Lesokhin et al., 1999)

eye disc morphogenetic furrow | ectopic, with Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

intestinal stem cell | somatic clone, with Scer\GAL4^NP5130

(Ren et al., 2010)

ommatidium, with Scer\GAL4^h-H10

(Lesokhin et al., 1999)

wing, with Scer\GAL4^32B

(Lesokhin et al., 1999)

wing vein, with Scer\GAL4^32B

(Lesokhin et al., 1999)

Detailed Description

Statement

Reference

Over-expression of Egfr^{1.A887T.Scer\UAS} in MARCM mutant clones (under the control of Scer\GAL4^esg-NP5130) induces intestinal stem cell proliferation.

(Ren et al., 2010)

Over-expression using Scer\GAL4^fkh.PH leads to invagination of salivary gland cells with slightly aberrant shapes and to an invagination hole that is too large. This leads to fully invaginated glands with a too large and aberrantly shaped lumen, although the individual and common ducts appear normal.

(Maybeck and Röper, 2009)

Expression of Egfr^{1.A887T.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 results in ectopic marginal furrow formation in the eye disc.

(Kumar and Moses, 2001)

When expression is driven by Scer\GAL4^32B extra vein tissue appears in the wing. When expression is driven by Scer\GAL4^h-H10 eyes show gaps in the array of ommatidia.

(Lesokhin et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^Act.PU/Egfr^1.A887T.UAS is an enhancer of oocyte associated follicular epithelium | somatic clone phenotype of aPKC^k06403

(Tian et al., 2023)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Egfr^1.A887T.UAS is a non-suppressor of intestinal stem cell | somatic clone phenotype of lin-28^Δ1

(Chen et al., 2015)

Other

Statement

Reference

Egfr^1.A887T.UAS, Scer\GAL4^Act.PU, l(2)gl⁴ has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Egfr^1.A887T.UAS, Scer\GAL4^Act.PU, aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Egfr^1.A887T.UAS, Rok^CAT-KG.UAS, Scer\GAL4^Act.PU, aPKC^k06403 has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in total cell number per clone and reduction in number of Dl-positive intestinal stem cells per clone seen in MARCM clones in the intestine of lin-28^Δ1 adults is not rescued by expression of Egfr^{1.A887T.Scer\UAS} under the control of Scer\GAL4^tub.PU.

(Chen et al., 2015)

Expression of Egfr^{1.A887T.Scer\UAS} under the control of Scer\GAL4^hs.PU partially restores cyst formation in Inx2^FA42 females (branched fusomes are observed in 77% of cases), but the formation of egg chambers is not rescued in these females.

(Mukai et al., 2011)

Knock-down of yki through co-expression of yki^{dsRNA.N.Scer\UAS} in Egfr^{1.A887T.Scer\UAS} MARCM mutant clones (under the control of Scer\GAL4^esg-NP5130) does not affect intestinal stem cell proliferation.

(Ren et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9534

w^*; P{Egfr.1.A887T.UAS}12-4/CyO, P{sevRas1.V12}FK1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EGFR^A887T

(Tian et al., 2023)

Egfr^{1.A887T.Scer\UAS}

Egfr^1.A887T.UAS

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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